Reversible, fluorescence-based optical sensor for hydrogen peroxide

Mills, Andrew; Tommons, Cheryl; Bailey, R. T.; Tedford, M. C.; Crilly, Peter J.

doi:10.1039/b618506a

Cited by 31 publications

(19 citation statements)

References 20 publications

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“…By maintaining a good diabetic control and implementing strategies including supplementation of antioxidant micronutrients (vitamins E and C, β-carotene) will reduce the oxidative stress and thus help in reducing the risk of chronic complications [8]. Several methods, such as titrimetry [5], spectrometry [9], chemiluminescence [10], fluorimetry [11], chromatography [12], and electrochemical techniques [13,14] have been reported for this purpose. Amongst these techniques, the electrochemical techniques are preferable, because of their simplicity, low cost, high sensitivity, rapidity, and selectivity.…”

Section: Introductionmentioning

confidence: 99%

An amperometric H2O2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode

et al. 2017

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The nanoparticles (NPs) of hemoglobin (Hb) were prepared by desolvation method and characterized by transmission electron microscopy (TEM), UV spectroscopy and Fourier-transform IR (FTIR) spectroscopy. An amperometric H2O2 biosensor was constructed by immobilizing HbNPs covalently on to a polycrystalline Au electrode (AuE). HbNPs/AuE were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) before and after immobilization of HbNPs. The HbNPs/AuE showed optimum response within 2.5 s at pH 6.5 in 0.1 M sodium phosphate buffer (PB) containing 100 μM H2O2 at 30°C, when operated at –0.2 V against Ag/AgCl. The HbNPs/AuE exhibited Vmax of 5.161 ± 0.1 μA cm−2 with apparent Michaelis-Menten constant (Km) of 0.1 ± 0.01 mM. The biosensor showed lower detection limit (1.0 μM), high sensitivity (129 ± 0.25 μA cm−2 mM−1) and wider linear range (1.0–1200 μM) for H2O2 as compared with earlier biosensors. The analytical recoveries of added H2O2 in serum (0.5 and 1.0 μM) were 97.77 and 98.01% respectively, within and between batch coefficients of variation (CV) were 3.16 and 3.36% respectively. There was a good correlation between sera H2O2 values obtained by standard enzymic colorimetric method and the present biosensor (correlation coefficient, R2 =0.99). The biosensor measured H2O2 level in sera of apparently healthy subjects and persons suffering from diabetes type II. The HbNPs/AuE lost 10% of its initial activity after 90 days of regular use, when stored dry at 4°C.

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Section: Introductionmentioning

confidence: 99%

An amperometric H2O2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode

et al. 2017

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“…Several spectroscopic techniques (fluorescence and chemiluminescence) and chromatographic techniques (high performance liquid chromatography coupled with peroxyoxalate chemiluminescence detection) have been used in the past for the determination of H 2 O 2 in living cells (Mills et al, 2007 ; Chen et al, 2009 ; Ahammad, 2013 ). Light induced production of H 2 O 2 have been measured in PSII membranes by oxidation of thiobenzamide with lactoperoxidase.…”

Section: Introductionmentioning

confidence: 99%

Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

et al. 2015

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Hydrogen peroxide (H2O2) is known to be generated in Photosystem II (PSII) via enzymatic and non-enzymatic pathways. Detection of H2O2 by different spectroscopic techniques has been explored, however its sensitive detection has always been a challenge in photosynthetic research. During the recent past, fluorescence probes such as Amplex Red (AR) has been used but is known to either lack specificity or limitation with respect to the minimum detection limit of H2O2. We have employed an electrochemical biosensor for real time monitoring of H2O2 generation at the level of sub-cellular organelles. The electrochemical biosensor comprises of counter electrode and working electrodes. The counter electrode is a platinum plate, while the working electrode is a mediator based catalytic amperometric biosensor device developed by the coating of a carbon electrode with osmium-horseradish peroxidase which acts as H2O2 detection sensor. In the current study, generation and kinetic behavior of H2O2 in PSII membranes have been studied under light illumination. Electrochemical detection of H2O2 using the catalytic amperometric biosensor device is claimed to serve as a promising technique for detection of H2O2 in photosynthetic cells and subcellular structures including PSII or thylakoid membranes. It can also provide a precise information on qualitative determination of H2O2 and thus can be widely used in photosynthetic research.

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“…The optical parameters for H 2 O 2 sensing materials are summarized in Table 1. Extensive studies have been reported on absorbance-based [23][24][25][26][27] and fluorescence-based [28][29][30][31][32] H 2 O 2 sensing method. The currently available optical sensing methods utilized the changes in absorbance or luminescence intensity as detecting signal.…”

Section: Resultsmentioning

confidence: 99%

Optical sensing of H_2O_2 based on red-shift of emission wavelength of carbon quantum dots

Chu¹,

Hsieh²,

Su³

2016

Opt. Mater. Express

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In this paper, the multicolor photoluminescence carbon quantum dots (CQDs) have been synthesized by one step hydrothermal method to analyze hydrogen peroxide (H 2 O 2 ). These CQDs exhibited multicolor emission under a single wavelength excitation for the quantification of H 2 O 2 . Using an LED with a central wavelength of 365 nm as the excitation source, it is shown that the red emission CQDs capable of detecting H 2 O 2 over the linear range of 0-88.2 mM and the H 2 O 2 sensitivity of wavelength shift to changes in the H 2 O 2 concentration was found to be 0.18 nm/mM. These results of the optical sensing method can be used in practice detection of H 2 O 2 and could offer a new approach for developing a new biosensor. The CQDs exhibit good emission property and high stability, as well as excitation-independent emission behavior. Moreover, it is attractive that CQDs can be used as an effective fluorescent probe for the detection of H 2 O 2 with linear Stern-Volmer plot and wavelength shift in an aqueous solution.

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Reversible, fluorescence-based optical sensor for hydrogen peroxide

Cited by 31 publications

References 20 publications

An amperometric H2O2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode

An amperometric H2O2 biosensor based on hemoglobin nanoparticles immobilized on to a gold electrode

Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

Optical sensing of H_2O_2 based on red-shift of emission wavelength of carbon quantum dots

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