Reversible fluorescence labeling of amino groups of protein using dansylaminomethylmaleic anhydride

Sakata, K.; Hamase, Kenji; Zaitsu, Kiyoshi

doi:10.1016/s1570-0232(01)00629-8

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“…Ellman's reagent is widely used for the analysis of thiols in biological samples via the determination of the liberated thionitrobenzoate anion [126]. HPLC with pre-column derivatization with fluorogenic reagents, such as ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) has become the most popular method for thiol analysis [127][128][129][130].…”

Section: Derivatizationmentioning

confidence: 99%

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Iwasaki

Saito

Nakano

et al. 2009

Journal of Chromatography B

101

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Section: Derivatizationmentioning

confidence: 99%

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Iwasaki

Saito

Nakano

et al. 2009

Journal of Chromatography B

101

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Applications of reversible covalent chemistry in analytical sample preparation

Siegel

2012

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Reversible covalent chemistry (RCC) adds another dimension to commonly used sample preparation techniques like solid-phase extraction (SPE), solid-phase microextraction (SPME), molecular imprinted polymers (MIPs) or immuno-affinity cleanup (IAC): chemical selectivity. By selecting analytes according to their covalent reactivity, sample complexity can be reduced significantly, resulting in enhanced analytical performance for low-abundance target analytes. This review gives a comprehensive overview of the applications of RCC in analytical sample preparation. The major reactions covered include reversible boronic ester formation, thiol-disulfide exchange and reversible hydrazone formation, targeting analyte groups like diols (sugars, glycoproteins and glycopeptides, catechols), thiols (cysteinyl-proteins and cysteinyl-peptides) and carbonyls (carbonylated proteins, mycotoxins). Their applications range from low abundance proteomics to reversible protein/peptide labelling to antibody chromatography to quantitative and qualitative food analysis. In discussing the potential of RCC, a special focus is on the conditions and restrictions of the utilized reaction chemistry.

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Reversible fluorescence labeling of amino groups of protein using dansylaminomethylmaleic anhydride

Cited by 2 publications

References 11 publications

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Chromatographic and mass spectrometric analysis of glutathione in biological samples

Applications of reversible covalent chemistry in analytical sample preparation

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