Reversible G1 arrest of a human Burkitt lymphoma cell line(Raji) induced by tunicamycin

Nishikawa, Yoshihisa; Yamamoto, Yasutake; Kaji, Koichi; Mitsui, Hiromi

doi:10.1016/s0006-291x(80)80007-6

Cited by 36 publications

(12 citation statements)

References 17 publications

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“…Stressed cells are usually growth-arrested at the G 1 phase of the cell cycle (40,41,43,64), as we demonstrate here for polyamine-depleted cells. The G 1 arrest in polyamine-depleted cells was associated with increased transcription of p21 and Gadd45a, decreased transcription of Cdk2, E2f1, Pcna, cyclins A and E, and most profoundly with decreased levels of the cyclin D1 protein, a regulator of the Cdks required for G 1 /S transition, whose decline was demonstrated to suffice for arresting cellular proliferation (40,65).…”

Section: Discussionsupporting

confidence: 68%

Expression Profiling and Biochemical Analysis Suggest Stress Response as a Potential Mechanism Inhibiting Proliferation of Polyamine-depleted Cells

Landau

Ran

Bercovich

et al. 2012

Journal of Biological Chemistry

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Background: Depletion of cellular polyamines results in cessation of cellular proliferation. Results: Genomic and biochemical analysis demonstrates stress establishment in the polyamine-depleted cells. Conclusion: Establishment of stress response accounts at least in part for growth arrest establishment in polyamine-depleted cells. Significance: Our results suggest a novel view on the mechanistic basis accounting for growth arrest of polyamine-depleted cells.

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Section: Discussionsupporting

confidence: 68%

Expression Profiling and Biochemical Analysis Suggest Stress Response as a Potential Mechanism Inhibiting Proliferation of Polyamine-depleted Cells

Landau

Ran

Bercovich

et al. 2012

Journal of Biological Chemistry

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“…protein synthesis Different results reveal that mitogenesis involves both mevalonate-dependent dolichol synthesis (Potter and Marshall, 1982) and N' glycosylation of newly synthesizedproteins (Niskikawa et al, 1980;Estevez et al, 1991). Such findings suggested to us that LOV action might reflect an impairment of dolichol synthesis, thereby restraining [3H]mannose incorporation into N' glycoproteins and mitogenesis.…”

Section: Lovastatin Does Not Affect N'-glycosylation Ormentioning

confidence: 82%

Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells

Ortiz

Goin

Alzaga

et al. 1995

Journal Cellular Physiology

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Lovastatin (LOV), a hydroxy-methylglutaryl-coenzyme A (HMGCoA) reductase competitive inhibitor, blocks epidermal growth factor (EGF)- or prostaglandin F2 alpha (PGF2 alpha)-induced mitogenesis in confluent resting Swiss 3T3 cells. This inhibition occurs even in the presence of insulin, which potentiates the action of these mitogens in such cells. LOV exerts its effect in a 2-80 microM concentration range, with both mitogens attaining 50% inhibition at 7.5 microM. LOV exerted its effect within 0-8 h following mitogenic induction. Mevanolactone (10-80 microM) in the presence of LOV could reverse LOV inhibition within a similar time period. LOV-induced blockage of PGF2 alpha response is reflected in a decrease in the rate of cell entry into S phase. Neither cholesterol, ubiquinone, nor dolichols of various lengths could revert LOV blockage. In EGF- or PGF2 alpha-stimulated cells, LOV did not inhibit [3H]leucine or [3H]mannose incorporation into proteins, while tunicamycin, an inhibitor of N' glycosylation, prevented this last phenomenon. Thus, it appears that LOV exerts its action neither by inhibiting unspecific protein synthesis nor by impairing the N' glycosylation process. These findings strongly suggest that either EGF or PGF2 alpha stimulations generate early cell cycle signals which induce mevalonate formation, N' glycoprotein synthesis, and proliferation. The causal relationship of these events to various mechanisms controlling the onset of DNA synthesis is also discussed.

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“…Previously, we found for the first time that the de novo synthesis of the lipidlinked oligosaccharide is necessary for eukaryotic cells to traverse the G1 phase by finding that the cell growth of the human Burkitt lymphoma cell line Raji was reversibly arrested at the G1 phase of the cell cycle by tunicamycin, which inhibits GlcNAc-1-phosphate transferase. 6) Subsequently, these results were confirmed for Saccharomyces cerevisiae (S. cerevisiae).…”

Section: )mentioning

confidence: 86%

Cloning and transcriptional expression of mouse mannosyltransferase IV/V cDNA, which is involved in the synthesis of lipid-linked oligosaccharides

Nishimura

Shimono

Yoshimoto

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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We cloned the mouse mannosyltransferase IV/V gene (mALG11) from FM3A cells by a bioinformatic approach. The ORF contained 1476 bp encoding 492 amino acids. The cloned mALG11 complemented the growth defect of the Saccharomyces cerevisiae ALG11Δ mutant. In addition, we detected a variant cDNA by alternate splicing that had an additional four-nucleotide ATGC insertion at base 276 of the ORF. Consequently the variant cDNA encoded a truncated protein with 92 amino acids, lacking the glycosyltransferase group-1 domain. The variant cDNA occurs in many mouse strains according to EST database searches. Moreover, we detected it in FM3A cDNA, but we did not detect any such variants in the human EST database or in HeLa cDNA, although human ALG11 (hALG11) genomic DNA has the same sequence around the intron–exon boundaries as those of mALG11 genomic DNA. Hence, we concluded that there is different transcriptional control mechanism between mALG11 and hALG11.

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Reversible G1 arrest of a human Burkitt lymphoma cell line(Raji) induced by tunicamycin

Cited by 36 publications

References 17 publications

Expression Profiling and Biochemical Analysis Suggest Stress Response as a Potential Mechanism Inhibiting Proliferation of Polyamine-depleted Cells

Expression Profiling and Biochemical Analysis Suggest Stress Response as a Potential Mechanism Inhibiting Proliferation of Polyamine-depleted Cells

Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells

Cloning and transcriptional expression of mouse mannosyltransferase IV/V cDNA, which is involved in the synthesis of lipid-linked oligosaccharides

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