Reversible inhibition of electron transfer in the ubiquinol

Brustovetsky, N.N.; Amerkhanov, Z. G.; Popova, E.; Konstantinov, Alexander A.

doi:10.1016/0014-5793(90)80708-q

Cited by 29 publications

(4 citation statements)

References 10 publications

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“…The data also demonstrate that in muscle tissues, as in liver [15,16], there is, in addition to uncoupling, at least one more mitochondrial response clearly related to thermoregulation, i.e. the changes in maximal respiratory rare observed in the presence of an artificial uncoupler.…”

Section: Resultsmentioning

confidence: 70%

Thermoregulatory, carboxyatractylate‐sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids

Brustovetsky

Gnutov

et al. 1992

FEBS Letters

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Thermoregulatory uncoupling of oxidative phosphorylation has been studied in heart and skeletal muscle mitochondria of ground squirrels. The respiratory rate ofmitochondria in the presence of oligornycin was found to be much higher in winter (in hibernating, arousing, or aroused animals) than in summer. This additional respiration is strongly (arousing animals) or completely (hibernating and aroused anhnals) inhibited by carboxyntractylate (CAtr) and bovine serum albumin (USA). The CAtr-and BSA-induced decreases in the rate of respiration arc accompanied by membrane potential increases, The rate of the CAtr-and BSA-sensitive respiration is proportional to the content of free fatty acids which, in the heart, dccrcascs in the order: arousing Z+ aroused = hibernating > summer animals. Maximal respiratory rates observed in the prcxncc of dinitrophenol (arousing > aroused > summer > hibernating animals) do not parallel the fatty acid Ievcl. It is assumed that some heat production in the winter animals is due to fatty acid-induced, ATP/ADP-antiportcr-mediated uncoupling in heart and skeletal muscle mitochondria. The peak of heat production during arousal after hibernation also includes some other stimulatory effect on mitochondrial respiration.

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Section: Resultsmentioning

confidence: 70%

Thermoregulatory, carboxyatractylate‐sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids

Brustovetsky

Gnutov

et al. 1992

FEBS Letters

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“…Phospholipase A2 activity also decreases (21), indicating that oxidation of lipids of the inner mitochondrial membrane is reduced. Specifically, electron transfer through the ubiquinol:cytochrome cl segment of the respiratory chain of liver mitochondria isolated from hibernating ground squirrels (Citellus undulatus) is inhibited by 70-80%o compared with mitochondria isolated from nonhibernating control animals (107).…”

Section: Effects Of Calorie Restriction On Mitochondrial Function Andmentioning

confidence: 99%

Oxidative damage and mitochondrial decay in aging.

Shigenaga

Hagen

Ames

1994

Proc. Natl. Acad. Sci. U.S.A.

1,879

1,108

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We argue for the critical role of oxidative damage in causing the mitochondrial dysfunction of aging. Oxidants generated by nito dria appear to be the major source of the oxidative lesions that accumulate with age. Several mitochondrial fumctins decline with age. The contributing factors include the intrinsic rate of proton leakage across the inner mitochondrial membrane (a correlate of oxidant formation), decreased membrane fluidity, and decreased levels and function of cardiolipin, which supports the function of many of the proteins of the inner mitochondrial membrane.

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“…It was shown in our group that arousal of hibernating ground squirrels is accompanied by at least two metabolic effects favorable for heat production: (i) activation of the respiratory electron transport chain between cytochromes b and cl, which is inhibited in mitochondria of hi~~ating animals [13]; and (ii) CAM-inhibited uncoupling [14]. We recently reported that arousal causes significant increase in the FFA level in mitochondria [SJ.…”

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confidence: 99%

Cyclosporin A suppression of uncoupling in liver mitochondria of ground squirrel during arousal from hibernation

Brustovetsky

Gnutov

et al. 1993

FEBS Letters

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Energy coupling parameters were studied in liver mitochondria of ground squirrel during arousal from hibernation. It was found that such mitochondria become uncoupled during incubation with phosphate in a salt medium. The uncoupling was revealed by respiration rate increase and membrane potential decrease in the presence of oligomycin. Both effects were reversed by addition of cyclosporin A. Under the same in vitro conditions, mitochondria from aroused (active) animals showed no uncoupling but could be uncoupled by addition of palmitate in the cyclosporin A-sensitive fashion. It is proposed that formation ofeyclosporin A-sensitive pores can be involved in urgent heat production in arousing hibernators.

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Reversible inhibition of electron transfer in the ubiquinol

Cited by 29 publications

References 10 publications

Thermoregulatory, carboxyatractylate‐sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids

Thermoregulatory, carboxyatractylate‐sensitive uncoupling in heart and skeletal muscle mitochondria of the ground squirrel correlates with the level of free fatty acids

Oxidative damage and mitochondrial decay in aging.

Cyclosporin A suppression of uncoupling in liver mitochondria of ground squirrel during arousal from hibernation

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