Reversible membrane deformations by straight DNA origami filaments

Franquelim, Henri G.; Dietz, Hendrik; Schwille, Petra

doi:10.1039/d0sm00150c

Cited by 46 publications

(47 citation statements)

References 90 publications

(116 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high‐density filaments could trigger the occurrence of spike‐like membrane protrusions on the GUV, which could be reversibly controlled by adjusting the assembly and depolymerization of filaments. [ 138 ] These results indicated that DNA nanostructures could mimic membrane proteins lacking inherent curvature to deform the membrane through the crowding mechanism.…”

Section: Mimic Membrane Deformation and Heterogeneous Membrane Fusionmentioning

confidence: 99%

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

Piao

Yuan

Dong

2021

Macromolecular Bioscience

View full text Add to dashboard Cite

Employing DNA nanostructures mimicking membrane proteins on artificial amphiphilic membranes have been widely developed to understand the structures and functions of the natural membrane systems. In this review, the recent developments in artificial systems constructed by amphiphilic membranes and DNA nanostructures are summarized. First, the preparations and properties of the amphipathic bilayer models are introduced. Second, the interactions are discussed between the membrane and the DNA nanostructures, as well as their coassembly behaviors. Next, the alternative systems related to membrane protein‐mediated signal transmission, selective distribution, transmembrane channels, and membrane fusion are also introduced. Moreover, the constructions of membrane skeleton protein‐mimicking DNA nanostructures are also highlighted.

show abstract

Section: Mimic Membrane Deformation and Heterogeneous Membrane Fusionmentioning

confidence: 99%

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

Piao

Yuan

Dong

2021

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Large flat sections appeared on the GUV with kinks at the phase boundaries between the polymerized flat DNA sheets. In addition to the morphological change, we observe a suppression of membrane fluctuations (Supplementary Figure S13, Video S5), indicating a mechanical stabilization of the compartment [35,43] by the DNA-based cytoskeleton mimic. Both the morphological and the mechanical alterations are reversible: Addition of a base led to pH decrease and hence to the detachment of the DNA origami from the GUV membrane.…”

Section: Ph-induced Morphology Changementioning

confidence: 88%

“…Instead of relying on purified proteins, an increasingly popular approach is to construct such pH-dependent machineries de novo from molecular building blocks. DNA nanotechnology, in particular, has been employed to build a variety of functional components for synthetic cells, [17,30,31] including membrane-sculpting [32][33][34][35] and pH-responsive components such as filaments [36] or rotors. [37,38] However, pH-responsive actuation is challenging after encapsulation into a compartment.…”

Section: Ph-sensitive Attachment Of Dna To the Compartment Peripherymentioning

confidence: 99%

Actuation of synthetic cells with proton gradients generated by light-harvesting E. coli

Jahnke

Ritzmann

Fichtler

et al. 2020

Preprint

View full text Add to dashboard Cite

Bottom-up and top-down approaches to synthetic biology each employ distinct methodologies with the common aim to harness new types of living systems. Both approaches, however, face their own challenges towards biotechnological and biomedical applications. Here, we realize a strategic merger to convert light into proton gradients for the actuation of synthetic cellular systems. We genetically engineer E. coli to overexpress the light-driven inward-directed proton pump xenorhodopsin and encapsulate them as organelle mimics in artificial cell-sized compartments. Exposing the compartments to light-dark cycles, we can reversibly switch the pH by almost one pH unit and employ these pH gradients to trigger the attachment of DNA structures to the compartment periphery. For this purpose, a DNA triplex motif serves as a nanomechanical switch responding to the pH-trigger of the E. coli. By attaching a polymerized DNA origami plate to the DNA triplex motif, we obtain a cytoskeleton mimic that considerably deforms lipid vesicles in a pH-responsive manner. We foresee that the combination of bottom-up and top down approaches is an efficient way to engineer synthetic cells as potent microreactors.

show abstract

“…In a more recent study, the same group developed straight origami DNA filaments that could reversibly deform GUVs. 180 DNA clathrin-mimics were also shown to cause deformations similar to clathrin-coated pits 181 and DNA nanosprings to cause GUV tubulation. 182 It has also been shown that DNA nanopores (discussed in a later section) cluster and remodel membranes into protrusions and can facilitate the formation of ultrathin lipid tubes.…”

Section: Applications At the Interface Of Dna Nanostructures And Lipimentioning

confidence: 99%

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

et al. 2020

View full text Add to dashboard Cite

DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.

show abstract

Reversible membrane deformations by straight DNA origami filaments

Abstract: Reversible MgCl2-induced blunt-end polymerization of membrane-bound straight DNA origami monomers into filaments leads to protruding deformations on freestanding lipid membranes.

Cited by 46 publications

References 90 publications

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

Recent Progress of DNA Nanostructures on Amphiphilic Membranes

Actuation of synthetic cells with proton gradients generated by light-harvesting E. coli

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

Contact Info

Product

Resources

About