Reversible mitochondrial uncoupling in the cold phase during liver preservation/reperfusion reduces oxidative injury in the rat model

Phosphoenolpyruvate, a glycolytic intermediate, as a cytoprotectant and antioxidant in ex-vivo cold-preserved mouse liver: a potential application for organ preservation

“…The increasing effect of PEP may be due to not only substrate‐level phosphorylation by the high‐energy bond but also to other mechanisms such as mitochondria activation. The protection of mitochondrial function seems to improve the energy metabolism and prevent cellular injury, including apoptosis and necrosis, during the cold preservation of the liver . Since oxidative stress is one of the major causes of mitochondrial dysfunction, the antioxidative properties of PEP may provide the mitochondria protection.…”

“…The protection of mitochondrial function seems to improve the energy metabolism and prevent cellular injury, including apoptosis and necrosis, during the cold preservation of the liver. [32,33] Since oxidative stress is one of the major causes of mitochondrial dysfunction, [34] the antioxidative properties of PEP may provide the mitochondria protection. Further studies to demonstrate this hypothesis, such as studying the effects of PEP on respiratory chain activity, uncoupling protein 2, superoxide dismutase 2 expression, cytochrome C release and mitochondria transition pore should also be performed.…”

Bioregulators of stem and progenitor cells in preservation solution reduce cold ischemia‐reperfusion injury of isolated rat livers

“…The preservation medium was sucrose-phosphate solution (SPS) developed in our laboratory (250 mM sucrose, 1 mM MgSO 4 , 0.5 mM CaCl 2 , 15 mM Na 2 HPO 4 , 30 mM KH 2 PO 4 , 1% polyethylene glycol 8000, pH 7.4 at 48C) [20,21].…”

“…After HS organs were reperfused at 378C for 60 min in a recirculating system with Krebs-Ringer bicarbonate solution (120 mM NaCl, 4.8 mM KCl, 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.2 mM MgSO 4 , 2.6 mM of CaCl 2 , 10 mM HEPES, 4.5 mM glucose, pH 7.4) saturated with 5% CO 2 . Flow rates 3-4.5 mL/mg of tissue/min were adjusted using 30-40 mm H 2 O pressure [20,21].…”

Organ Preservation into the 2020s: The Era of Dynamic Intervention

“…Mild uncoupling of oxidative phosphorylation is an approach to preventing hyperpolarization of the mitochondrial membrane. It has been proposed that the use of water-soluble uncoupler of oxidative phosphorylation 2,4-dinitrophenol (DNP) as a component of cold preservation solution will make it possible to lower oxidative injury of the liver [92]. Indeed, sup­plementation of sucrose-based cold solution with DNP resulted in decreasing ROS production in isolated livers after cold storage, uncoupler washout and, following normothermic reperfusion, preventing the inhibition of antioxidant enzyme activities, and improving morphology and bile secretion of the liver.…”