Reversible protein precipitation to ensure stability during encapsulation within PLGA microspheres

Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection

“…Alternatively, the s/o/w procedure has also been proposed, predominantly for hydrophilic proteins. 30 Using these approaches, drug entrapment levels observed in this study broadly agreed with the results of previous studies. 12 However, to increase entrapment efficiency, we examined the effect of altering the pH of the external aqueous phase.…”

Evaluation of biodegradable polyester-co-lactone microparticles for protein delivery

“…The higher M W polymer, PGA-co-PDL (1:1:1, 39.0 KDa), and the more hydrophobic polymers, PGA-co-PDL (1:1:1.45) and PPA-co-PDL, showed a significantly (p50.05) higher bioactive fraction, after 5 and 24 h release, compared to the other co-polymers. The maximum LS bioactive fraction was found using PGA-co-PDL LS is a relatively stable enzyme 55 which can better withstand the harsh condition of the emulsification process and this was confirmed by the retention of its bioactivity at zero time of release (bioactive fraction ranged from 0.9 to 1.03 for all the investigated polymers, Figure 7). Similarly, it was reported by Giteau and coworkers that the LS released from PLGA microspheres was still biologically active compared to a-CH, peroxidase and b-galactosidase-loaded PLGA microspheres 57…”

Improved Lysozyme Stability and Release Properties of Poly(lactide-co-glycolide) Implants Prepared by Hot-Melt Extrusion

“…Although lysozyme has sometimes been referred to be a relatively stable protein, its low recovery in the presence of PLGA was remarkable (3,8). The low recovery of lysozyme as well as its incomplete release from PLGA-based delivery systems has been related to the protein instability during manufacturing of the delivery system and during release (2,4,9).…”

“…Lysozyme is a popular model protein in pharmaceutical research, and its incorporation into PLGA-based delivery systems has been reported extensively (2)(3)(4)(5)(6)(7). Although lysozyme has sometimes been referred to be a relatively stable protein, its low recovery in the presence of PLGA was remarkable (3,8).…”