Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior

Abstract

Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2‑4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.

Chiral supramolecular nanomaterials: From chirality transfer and amplification to regulation and applications

“…[79,80] Yeung et al have reported a technique that relies on supramolecular interactions to generate reversible selfassembled monolayers characterized by tunable lateral mobility and the capacity to dynamically manipulate surface composition, thereby governing cell adhesion behavior. [81] The sensing mechanism employed by this system entails the utilization of noncovalent ion pairs between amidinium and carboxylate to facilitate the creation (on) and dissolution (off) of stable 2D structures. These structures bear a resemblance to lipid bilayers but can be easily prepared and exhibit rapid on/off rates, as depicted in Figure 5A.…”

“…Reproduced with permission. [ 81 ] Copyright 2022, with permission from American Chemical Society. (B) Circular dichroism spectra, schematic illustrations, and fluorescence images for 3D MSA structures.…”

Strategy for Nonenzymatic Harvesting of Cells via Decoupling of Adhesive and Disjoining Domains of Nanostructured Stimulus-Responsive Polymer Films

“…These mechanical problems restrain the scalability of the mechanical methods. Chemical and biochemical methods of cell harvesting based on changes in pH of the medium, , ion concentration, electrochemical processes, − reversible self-assembled monolayers, reversible or competitive molecular recognition, − and light-induced photochemical processes , are limited due to the high sensitivity of cells to the composition of the medium or high costs of the biomaterials. Even minor changes in the medium’s chemical composition, concentrations, and pH can trigger cell apoptosis or mutations.…”

Nanoparticles with adaptable self-assembled shells sense and inhibit influenza virus at ultra-low concentrations

“…1). rSAMs offer robust and adaptable receptors featuring strongly enhanced a nities toward proteins, 29 viruses 30 and human cells 31 compared to receptors based on stiffer scaffolds. The formation of the rSAMs is driven by the spontaneous assembly of benzamidineterminated amphiphiles on alkanoic acid functionalized surfaces (e.g.…”