2024
DOI: 10.1007/s40766-024-00052-1
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Reversibly switchable fluorescent proteins: “the fair switch project”

Riccardo Nifosì,
Barbara Storti,
Ranieri Bizzarri

Abstract: Fluorescent proteins (FPs) have transformed cell biology through their use in fluorescence microscopy, enabling precise labeling of proteins via genetic fusion. A key advancement is altering primary sequences to customize their photophysical properties for specific imaging needs. A particularly notable family of engineered mutants is constituted by Reversible Switching Fluorescent Proteins (RSFPs), i.e. variant whose optical properties can be toggled between a bright and a dark state, thereby adding a further … Show more

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Cited by 2 publications
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