Reversion of Yeast Protoplasts in Media containing Polyethylene Glycol

Svoboda, Augustín; Piedra, D

doi:10.1099/00221287-129-11-3371

Cited by 8 publications

(8 citation statements)

References 7 publications

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“…As previously described (Svoboda and Piedra, 1983;Svoboda and Necas, 1987;Makarow, 1988;Preuss et al, 19821, wild-type S. cerevisiae and sec7 mutants grown at 24°C do not contain structures corresponding to the elaborate Golgi apparatus observed in mammalian cells. In wild-type s. cerevisiae, Golgi compartments have been identified by immunoelectron microscopy and shown to consist of isolated cisternae, randomly distributed throughout the cytoplasm and usually not arranged into stacks of parallel cisternae or saccules (Preuss et al, 1992).…”

Section: Discussionmentioning

confidence: 59%

“…Such stacks of saccules, however, were rarely observed in wild-type cells (Svoboda and Piedra, 1983;Makarow, 1988). Thus in wild type of S. cerevisiae (Preuss et al, 1992) as well as in the sec7 mutant grown at permissive temperature (Svoboda and Necas, 19871, the Golgi apparatus consisted mainly of isolated cisternae or saccules dispersed throughout the cytoplasm but without connection between them.…”

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confidence: 95%

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Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Rambourg¹,

Clermont

Képès³

1993

Anat. Rec.

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The three-dimensional configuration of the Golgi apparatus has been examined with the electron microscope in thick Golgi sections of Saccharomyces cerevisiae prepared from a wild-type strain and from sec7 mutants maintained for various periods of time at the nonpermissive temperature of 37 degrees C and then returned to the permissive temperature of 24 degrees C. Reduced osmium postfixation of glutaraldehyde fixed specimens stained intensely the content of Golgi elements and thus facilitated their three-dimensional characterization. In wild-type S. cerevisiae, the Golgi elements usually appeared as isolated networks of membranous tubules dispersed throughout the cytoplasm. Along such networks, distensions filled with stained material were similar in size to nearby secretory granules, suggesting that the latter formed by fragmentation of the Golgi elements. In sec7 mutants maintained at 37 degrees C in low (0.1%) glucose medium, secretion granules progressively decreased in number and soon disappeared. Concomitantly the networks of Golgi tubules increased in size and complexity, lost their distensions, and then transformed into flattened saccules forming stacks of up to seven or eight saccules that were similar to the Golgi stacks seen in mammalian cells. However in contrast to the latter, connections between the saccules were evident and Golgi-associated small vesicles were generally absent. Following return to the permissive temperature (24 degrees C), secretion granules reappeared, the Golgi stacks progressively decreased in size, and resumed their initial state of separated small tubular networks. Thus in sec7 mutant, grown at 37 degrees C in low glucose medium, segregation of secretory granules is blocked. As a result, Golgi membranes accumulate to form a continuous system of stacked and interconnected saccules.

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Section: Discussionmentioning

confidence: 59%

mentioning

confidence: 95%

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Rambourg¹,

Clermont

Képès³

1993

Anat. Rec.

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“…Indeed, with careful sample preparation, stacked cisternae consisting of two to four membranous compartments can be found in S. cerevisiae cells (Moor and Muhlethaler, 1963;Svoboda and Piedra, 1983;Makarow, 1988), and similar compartments have been observed near the projections of cells responding to the mating pheromone, a-factor (Baba et al, 1989). More definitive Golgi structures, reminiscent of those found in mammalian cells, are apparent in strains with mutations that disrupt traffic through the secretory pathway (Novick et al, 1980Svoboda and Necas, 1987).…”

Section: Introductionmentioning

confidence: 84%

“…Conventional electron microscopy preparations have revealed few structures corresponding closely to the elaborate Golgi complexes found in higher cells (Svoboda and Piedra, 1983;Svoboda and Necas, 1987), yet yeast clearly have Golgi functions, as summarized below. Animal cells typically contain one Golgi complex (Novikoff et al, 1971;Rambourg et al, 1981) that consists of several parallel and functionally distinct membranous sacs in which the oligosaccharides that decorate secretory proteins are successively extended and modified (reviewed in Kornfeld and Kornfeld 1985).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.

Preuss

Mulholland

Franzusoff

et al. 1992

MBoC

263

221

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The membrane compartments responsible for Golgi functions in wild-type Saccharomyces cerevisiae were identified and characterized by immunoelectron microscopy. Using improved fixation methods, Golgi compartments were identified by labeling with antibodies specific for alpha 1-6 mannose linkages, the Sec7 protein, or the Ypt1 protein. The compartments labeled by each of these antibodies appear as disk-like structures that are apparently surrounded by small vesicles. Yeast Golgi typically are seen as single, isolated cisternae, generally not arranged into parallel stacks. The location of the Golgi structures was monitored by immunoelectron microscopy through the yeast cell cycle. Several Golgi compartments, apparently randomly distributed, were always observed in mother cells. During the initiation of new daughter cells, additional Golgi structures cluster just below the site of bud emergence. These Golgi enter daughter cells at an early stage, raising the possibility that much of the bud's growth might be due to secretory vesicles formed as well as consumed entirely within the daughter. During cytokinesis, the Golgi compartments are concentrated near the site of cell wall synthesis. Clustering of Golgi both at the site of bud formation and at the cell septum suggests that these organelles might be directed toward sites of rapid cell surface growth.

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“…The yeast S. cerevisiae see21 mutant, maintained at a permissive temperature, like wild type cells, contains several Golgi elements widely dispersed throughout the cytoplasm (Svoboda and Piedra, 1983;Svoboda and Necas, 1987;Makarow, 1988;Preuss et al, 1992). These elements, when examined in electron microscope, stereoscopic images of thick sections appear as isolated tubular networks (Rambourg et al, 1993) (Fig.…”

Section: Structure Of Golgi Elements In Sec2l Mutant At Permissive Tementioning

confidence: 99%

Ultrastructural modifications of vesicular and Golgi elements in the Saccharomyces cerevisiae sec21 mutant at permissive and non‐permissive temperatures

Rambourg¹,

Clermont

Jackson³

et al. 1994

Anat. Rec.

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Reversion of Yeast Protoplasts in Media containing Polyethylene Glycol

Cited by 8 publications

References 7 publications

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.

Ultrastructural modifications of vesicular and Golgi elements in the Saccharomyces cerevisiae sec21 mutant at permissive and non‐permissive temperatures

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