Background: Uric acid is a metabolic process produced by each individual through the breakdown of purines carried out in the liver. Increased level of uric acid in blood is called hyperuricemia, which might cause gout if it is not treated properly. Lemon (Citrus Limon.) contains flavonoids which act as antioxidants and have the potential as antihyperuricemia Aim: This paper aimed to study the effect of lemon seed extract on uric acid levels Methods: Thirty-six male wistar rats from Farmhouse Ungaran were randomly divided into 6 groups, consists of healthy control group, hyperuricemic control, Allopurinol control, and treatment groups (3 groups). Hyperuricemia was induced by the administration of block broth and potassium oxonate for 4 weeks. Lemon seed extract doses of 17,5, 35, and 70 mg/kg BW, and Allopurinol 90 mg/kg BW was given orally for 2 weeks. Statistical analysis was conducted to evaluate differences among groups before and after the intervention. Results: There was a significant difference (p<0,05) in uric acid levels before and after intervention of lemon peel extract in groups K1, K2, and P3. Meanwhile, in groups K0, P1, and P2, there were no significant differences. In the pre-test uric acid levels, there was a significant difference (p<0,05) between the K0 group and K1, K2, P1, P2, and P3. Conclusion: There were no significant differences in the uric level acid of the treatment and control group after intervention, but there were significant differences in the effects of graded doses between the treatment groups.