2023
DOI: 10.1002/etc.5648
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Review and Development of Best Practices for Toxicity Tests with Dreissenid Mussels

Abstract: Since their introduction to North America in the 1980s, research to develop effective control tools for invasive mussels (Dreissena polymorpha and D. rostriformis bugensis) has been ongoing across various research institutions using a range of testing methods. Inconsistencies in experimental methods and reporting present challenges for comparing data, repeating experiments, and applying results. The Invasive Mussel Collaborative established the Toxicity Testing Work Group (TTWG) in 2019 to identify “best pract… Show more

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Cited by 4 publications
(2 citation statements)
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“…However, we failed to collect the necessary data to investigate mussel ion balance and how it may have affected dose-response in the present study, but it highlights a potential confounding factor for laboratory testing with mussels. Recent suggestions from an Invasive Mussel Collaborative work group are to hold mussels for a minimum of 7 days to observe mortality and then hold them for an additional 48 h after they have acclimated to test conditions (Waller et al, 2023). Potassium chloride and CO 2 toxicity are likely not directly comparable because of differing modes of action.…”
Section: Discussionmentioning
confidence: 99%
“…However, we failed to collect the necessary data to investigate mussel ion balance and how it may have affected dose-response in the present study, but it highlights a potential confounding factor for laboratory testing with mussels. Recent suggestions from an Invasive Mussel Collaborative work group are to hold mussels for a minimum of 7 days to observe mortality and then hold them for an additional 48 h after they have acclimated to test conditions (Waller et al, 2023). Potassium chloride and CO 2 toxicity are likely not directly comparable because of differing modes of action.…”
Section: Discussionmentioning
confidence: 99%
“…A wide variety of methods have been developed to assess toxicology in dreissenid mussels and efforts are currently underway to harmonize these methods so that comparisons can be made more easily between laboratories. 186 Our groups are also currently working on establishing quantitative phenotypic assays for attachment, filtration of food particles, growth, reproduction, and survival. In addition, the assessment of molecular phenotypes by RT-qPCR or RNA-sequencing will also provide valuable insights into the efficacy of genetic manipulations.…”
Section: Limitations and Barriers To Applying Genetic Biocontrol Tool...mentioning
confidence: 99%