Review and Prospect for Single Molecule Localization Microscopy

Yuzhu, 李雨竹 Li; Chuankang, 李传康 Li; Hao, Xiang; Xu, 刘旭 Liu; Cuifang, 匡翠方 Kuang

doi:10.3788/lop57.240002

Cited by 3 publications

(2 citation statements)

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“…20,21 As a result, when two imaging objects are close together in the scene, Airy patterns overlap together and cannot be distinguished. 22,23 According to the Rayleigh criterion, it is just possible to distinguish the image formed by two points when the center of one Airy disk overlaps with the first-order dark ring of another Airy disk. At this time, the distance between the center of the two Airy patterns is equal to the radius of the Airy disk.…”

Section: Principles Of Single-molecule Localization Microscopymentioning

confidence: 99%

“…When a single-point light source passes through an optical imaging system, it will be affected by optical diffraction. This makes the resulting image a diffuse pattern rather than a point of light. , This pattern consists of a bright center surrounded by a series of diffraction rings with wanning brightness, called an Airy disk, also known as the point spread function (PSF). , As a result, when two imaging objects are close together in the scene, Airy patterns overlap together and cannot be distinguished. , According to the Rayleigh criterion, it is just possible to distinguish the image formed by two points when the center of one Airy disk overlaps with the first-order dark ring of another Airy disk. At this time, the distance between the center of the two Airy patterns is equal to the radius of the Airy disk .…”

Section: Principles Of Single-molecule Localization Microscopymentioning

confidence: 99%

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Nucleic Acid Probes for Single-Molecule Localization Imaging of Cellular Biomolecules

Wei

Wang

et al. 2023

Chemical & Biomedical Imaging

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Endogenous biomolecules in cells are the basis of all life activities. Directly visualizing the structural characteristics and dynamic behaviors of cellular biomolecules is significant for understanding the molecular mechanisms in various biological processes. Single-molecule localization microscopy (SMLM) can circumvent the optical diffraction limit, achieving analysis of the fine structures and biological processes in living cells with nanoscale resolution. However, the large size of traditional imaging probes prevents SMLM from accurately locating fine structures and densely distributed biomolecules within cells. In recent years, nucleic acid probes have emerged as potential tools to replace conventional SMLM probes by virtue of their small size and high specificity. In addition, due to their programmability, nucleic acid probes with different conformations can be constructed via sequence design, further extending the application of SMLM in bioanalysis. Here, we discuss the design concepts of different conformational nucleic acid probes for SMLM and summarize the application of SMLM based on nucleic acid probes in the field of biomolecules. Furthermore, we provide a summary and future perspectives of the nucleic acid probe-based SMLM technology, aiming to provide guidance for the acquisition of nanoscale information about cellular biological processes.

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Section: Principles Of Single-molecule Localization Microscopymentioning

confidence: 99%