Review and Recent Development of Angle-Resolved Low-Coherence Interferometry for Detection of Precancerous Cells in Human Esophageal Epithelium

Brown, William J.; Pyhtila, John W.; Terry, Neil G.; Chalut, Kevin J.; D’Amico, Thomas A.; Sporn, Thomas A.; Obando, Jorge; Wax, Adam

doi:10.1109/jstqe.2007.913969

Cited by 43 publications

(56 citation statements)

References 20 publications

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“…A detailed discussion of this methodology is presented in Brown et al [18]. First, the a/LCI scan is segmented into depth bins, and the angular scattering distribution is averaged within each depth bin for analysis.…”

Section: Mie Theory Ilsa Processingmentioning

confidence: 99%

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium

Drake

Bentley

et al. 2015

Biomed. Opt. Express

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Abstract:We evaluate a new hybrid algorithm for determining nuclear morphology using angle-resolved low coherence interferometry (a/LCI) measurements in ex vivo cervical tissue. The algorithm combines Mie theory based and continuous wavelet transform inverse light scattering analysis. The hybrid algorithm was validated and compared to traditional Mie theory based analysis using an ex vivo tissue data set. The hybrid algorithm achieved 100% agreement with pathology in distinguishing dysplastic and non-dysplastic biopsy sites in the pilot study. Significantly, the new algorithm performed over four times faster than traditional Mie theory based analysis.

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Section: Mie Theory Ilsa Processingmentioning

confidence: 99%

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium

Drake

Bentley

et al. 2015

Biomed. Opt. Express

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“…Then the chi-squared value of the best fit is compared that of the next best fit. If the best fit value is not smaller than the second best fit by more than 10%, the fit is determined to be a non-unique solution and discarded [2]. Both the properly oriented and folded samples passed the first test criterion in the comparison with the null solution, indicating that useful scattering information was obtained.…”

Section: Discussionmentioning

confidence: 99%

“…To reveal the structure of the cell nuclei more precisely, the 2D angular scattering measurements can be analyzed using a theoretical model such as Mie theory [2] or T-matrix based analysis [23]. While T-matrix based analysis can give size, shape, and orientation of scatterers by treating scatterers as spheroids instead of spheres as in Mie theory, it requires significant computation time.…”

Section: Rat Esophagusmentioning

confidence: 99%

“…The a/LCI system operates by collecting elastically scattered light from biological samples for various scattering angles and utilizes interferometry to depth gate the measurements. The angular scattering distribution arising from selected depths within the sample can then be analyzed to determine quantitative depthresolved nuclear morphology measurements [2]. The nuclear morphology data, specifically enlargement of the nuclei in the basal layer of the epithelium, have been shown to be a highly useful biomarker for the early detection of dysplasia, a precancerous tissue state.…”

Section: Introductionmentioning

confidence: 99%

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Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography

Kim

Heflin

Kresty

et al. 2016

Biomed. Opt. Express

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Angle-resolved low coherence interferometry (a/LCI) is an optical technique used to measure nuclear morphology in situ. However, a/LCI is not an imaging modality and can produce ambiguous results when the measurements are not properly oriented to the tissue architecture. Here we present a 2D a/LCI system which incorporates optical coherence tomography imaging to guide the measurements. System design and characterization are presented, along with example cases which demonstrate the utility of the combined measurements. In addition, future development and applications of this dual modality approach are discussed. and T. E. Milner, "Dual-modality fiber-based OCT-TPL imaging system for simultaneous microstructural and molecular analysis of atherosclerotic plaques," Biomed. Opt. Express 6(5), 1665-1678 (2015). 12. T. Liu, Q. Wei, J. Wang, S. Jiao, and H. F. Zhang, "Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen," Biomed. Opt. Express 2(5), 1359-1365 (2011). 13. A. L. Clark, A. Gillenwater, R. Alizadeh-Naderi, A. K. El-Naggar, and R. Richards-Kortum, "Detection and diagnosis of oral neoplasia with an optical coherence microscope," J. Biomed. Opt. 9(6), 1271-1280 (2004). 14. T. S. Ralston, D. L. Marks, P. S. Carney, and S. A. Boppart, "Interferometric synthetic aperture microscopy," Nat. Phys. 3(2 Sidman, "Morphological, physiological, and biochemical changes in rhodopsin knockout mice," Proc. Natl. Acad. Sci. U.S.A. 96(2), 736-741 (1999). 20. J. W. Pyhtila, H. Ma, A. J. Simnick, A. Chilkoti, and A. Wax, "Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry," J. Biomed. Opt. 11(3), 034022 (2006). 21. A. Wax and J. W. Pyhtila, "In situ nuclear morphology measurements using light scattering as biomarkers of neoplastic change in animal models of carcinogenesis," Dis. Markers 25(6), 291-301 (2008).

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“…The angular distributions are compared to a theoretical basis, such as Mie theory in order to assess the most likely scattering geometry [26,29]. It has been shown that the angular distribution of the scattered intensity is related to the two-point spatial correlation function of the optical field through a Fourier transform [14] E( θ)…”

Section: Signal Analysismentioning

confidence: 99%

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

Wax

Chalut

2011

Analytical Cellular Pathology

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Abstract. The study of intact, living cells using non-invasive optical spectroscopic methods offers the opportunity to assess cellular structure and organization in a way that is not possible with commonly used cell biology imaging techniques. We have developed a novel spectroscopic technique for diagnosing disease at the cellular level based on using low-coherence interferometry (LCI) to detect the angular distribution of scattered light. Angle-resolved LCI (a/LCI) combines the ability of LCI to isolate scattering from sub-surface tissue layers with the ability of light scattering spectroscopy to obtain structural information on sub-wavelength scales. In application to examining cellular structure, a/LCI enables quantitative measurements of changes in the size and texture of cell nuclei. These quantitative measurements are characteristic of different pathological states. The capabilities of a/LCI were demonstrated using a clinical system that can be applied in endoscopic surveillance of esophageal tissue, producing high sensitivity and specificity for detecting dysplastic tissues in vivo. Experiments with in vitro cell samples also show the utility of a/LCI in observing structural changes due to environmental stimuli as well as detecting apoptosis due to chemotherapeutic agents.

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Review and Recent Development of Angle-Resolved Low-Coherence Interferometry for Detection of Precancerous Cells in Human Esophageal Epithelium

Cited by 43 publications

References 20 publications

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium

Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography

Nuclear Morphology Measurements with Angle-Resolved Low Coherence Interferometry For Application To Cell Biology And Early Cancer Detection

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