Review: Evaluation of bull fertility. Functional and molecular approaches

Bollwein, Heinrich; Malama, Eleni

doi:10.1016/j.animal.2023.100795

Cited by 8 publications

(3 citation statements)

References 168 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evaluation of male fertility and the effectiveness of various assisted reproductive procedures, including sperm processing, in vitro embryo production and artificial insemination, depend heavily on the precise characterization of sperm quality (Bollwein & Malama, 2023). The current results revealed that spermatozoa motility, count, viability and normal morphology were elevated in the high-fertile group compared to the lower one.…”

Section: Discussionmentioning

confidence: 63%

Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

Rashad,

Ibrahim,

El‐Sokary

et al. 2024

Reprod Domestic Animals

View full text Add to dashboard Cite

The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes (PLA2G4D, LCN15, CLUAP1, SPP1, AQP12B, DEFB110 and ESR1) relevant to spermatozoa features between the different epididymal segments in the mature dromedary camels (n = 30). Scrotal contents were collected post‐slaughtering, during the breeding season and the epididymis was separated from the testicles and divided into three segments (caput, corpus and cauda) based on its morphology and anatomical characteristics. Epididymal spermatozoa were harvested from each epididymal portion and evaluated for motility, count, viability and morphology. Samples were grouped depending on their epididymal sperm cells features into high‐fertile (n = 15) and low‐fertile (n = 15) groups. The gene expression of the candidate genes was defined in the isolated RNA from each epididymal portion tissue. The segmental sperm motion and count were significantly (p < .05 and p < .01) higher in the three epididymal parts of high‐fertile camels than the lower ones. There were some candidate genes markedly up‐regulated in its expression in epididymal head of high‐fertile camels (PLA2G4D and LCN15) and low fertile (CLUAP1), while others in the body region of the high‐fertile group (SPP1, AQP12B and DEFB110). Nevertheless, ER1 did not differ in the expression among the epididymal segments. In conclusion, the variant expression patterns of these epididymal genes in relation to the regional spermatozoa features might suggest important roles of these genes in sperm maturation process in the epididymis and focusing more interest on their potential utility as markers for male camel fertility prediction.

show abstract

Section: Discussionmentioning

confidence: 63%

Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

Rashad,

Ibrahim,

El‐Sokary

et al. 2024

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Moreover, in the study of Rajamanickam et al (2017b) the HF bulls had increased tyrosine phosphorylation than LF bulls. Since regulation of Na/K-ATPase activity is observed during capacitation (Jimenez et al, 2012), and it was observed that capacitation increased Na/K-ATPase content and activity in bull sperm, apparently through mitochondrial translation (Rajamanickam et al, 2017a); further than differences between measurement technique, species, microenvironment, and reproductive strategies adopted that influence bull fertility (Bollwein and Malama, 2023;Kathiravan et al, 2011;Harstine et al, 2018), it is plausible that sperm pre-selection using percoll and assessment of sperm traits in a specific subpopulation (membrane-intact sperm) explain the differences between the report of Rajamanickam et al (2017b) and the present data.…”

Section: Discussionmentioning

confidence: 99%

“…However, a relationship between membrane integrity and fertility is commonly reported (Christensen et al, 2011;Kumaresan et al, 2017). Those discrepancies highlight the importance of associating several sperm traits to better predict a sire's fertility (Kumaresan et al, 2017;Bollwein and Malama, 2023;Muhammad Aslam et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Relationship between Na/K-ATPase in thawed sperm and fertility of Angus bulls

Marques,

Cezar,

Nascimento Júnior

et al. 2023

Anim. Reprod.

View full text Add to dashboard Cite

Since bull fertility prediction remains challenging, the identification of potential fertility markers is important considering the economic benefits to the livestock industry. The main goal of this study was to determine the Na/K-ATPase activity and expression in thawed sperm of high (HF)-and low-fertility (LF) Angus bulls. Samples from three different batches/bulls with HF (n = 4) and LF (n = 4) were used. The Na/K-ATPase activity was determined after thawing, whereas sperm kinematics, membrane integrity, and expression of Na/K-ATPase on sperm surface were evaluated immediately post-thaw and after 120 minutes of incubation. Within the same incubation time, there was no difference on sperm membrane integrity, kinematics, and the expression of Na/K-ATPase on the sperm surface between HF and LF bulls. Kinematic parameters of LIN and VCL were not influenced by incubation time in samples from HF and LF, respectively. A tendency (P = 0.06) of higher Na/K-ATPase enzymatic activity for sperm of HF bulls compared to LF bulls was observed (0.49 ± 0.07 and 0.32 ± 0.06, respectively). In conclusion, Na/K-ATPase activity and expression in thawed sperm from Angus bulls are not related to the fertility index after fixedtime artificial insemination. However, sperm kinematics related to hyperactivation might indicate higher sperm cryotolerance for HF bulls.

show abstract

A combination of biomarkers for predicting stallion sperm fertility

Johannisson,

Morrell,

Ntallaris

2024

Vet Res Commun

View full text Add to dashboard Cite

Equine breeding would benefit greatly from reliable biomarkers of stallion or ejaculate fertility. The aim of the study was to investigate how several in vitro sperm characteristics correlate with fertility after artificial insemination, to explore the potential to build a fertility prediction model for stallions. Cooled insemination doses (3–5 per stallion) were obtained from various studs. Sperm membrane integrity, acrosome integrity, chromatin integrity, mitochondrial membrane potential, and reactive oxygen species production were evaluated by flow cytometry 24–30 h after semen collection, and sperm motility was assessed by computer aided sperm analysis. Calcein violet was used to differentiate viable spermatozoa. Per season pregnancy rates for these stallions were available the following year. Positive correlations were found between pregnancy rate and straightness (r = 0.43, p ≤ 0.001), as well as pregnancy rate and the proportion of living hydrogen peroxide positive spermatozoa (r = 0.32, p ≤ 0.05). There were negative correlations between pregnancy rate and amplitude of lateral head displacement (r = -0.26, p ≤ 0.05), and between pregnancy rate and the mean fluorescence of dead superoxide positive spermatozoa (r = -0.46, p < 0.001). Principal component analysis indicated that motility, membrane integrity, DNA fragmentation, and reactive oxygen species production were associated with pregnancy rate. Therefore, a combination of these factors could be used as a biomarker of fertility when assessing ejaculates. However, data from more individuals would be required to construct a model for fertility prediction.

show abstract

Review: Evaluation of bull fertility. Functional and molecular approaches

Cited by 8 publications

References 168 publications

Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

Region‐specific gene expression profile in the epididymis of high‐ and low‐fertile dromedary camels

Relationship between Na/K-ATPase in thawed sperm and fertility of Angus bulls

A combination of biomarkers for predicting stallion sperm fertility

Contact Info

Product

Resources

About