Cite this article as: A. Garber, P.M. Hastie, I. Handel and M.D. Murray, In vitro fermentation of different ratios of alfalfa and starch or inulin incubated with an equine faecal inoculum, Livestock Science, http://dx.doi.org/10. 1016/j.livsci.2017.05.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. a further set of bottles containing identical ratios of S/I:CA/GA were also prepared, with the exception that the alfalfa received a simulated foregut digestion treatment (SFD) as prior to incubation. Total gas production increased (P<0.05) as the ratio of S/I to alfalfa increased.Total gas production was lower in bottles containing SFD-treated alfalfa (P<0.001). Dry matter loss decreased proportionately with increasing level of alfalfa substitution of S/I (P<0.001). Values for pH were lower in bottles containing S or I, with pH values in bottles 2 containing S alone falling to almost 6 and those with I dropping to pH 5 and under.However, the substitution of S or I with 40 percent alfalfa produced pH values above 6.7, which is within physiological levels encountered in the large intestine of the horse.Consequently, there appears to be considerable potential to buffer the deleterious effects of high-starch/fructan diets with the substitution of these substrates with high-temperature dried alfalfa.