Review: genetically modified plants for the promotion of human health

Yonekura‐Sakakibara, Keiko; Saito, Kazuki

doi:10.1007/s10529-006-9194-4

Cited by 61 publications

(29 citation statements)

References 41 publications

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“…It is known that the immunogenic proteins of the majority of pathogens can be synthesized in plant tissues, which can then be used as edible subunit vaccines. The effectiveness of this approach has been repeatedly confirmed [7][8][9][10].…”

mentioning

confidence: 75%

Expression of the nucleotide sequence for the M2e peptide of avian influenza virus in transgenic tobacco plants

Tarasenko

Taranov

Фирсов

et al. 2013

Appl Biochem Microbiol

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The M2e peptide of the H5N1 A/Chicken/Kurgan/05/2005 avian influenza virus was successfully synthesized in transgenic tobacco plants. The amino terminal segment of the M2 protein, comprised of 22, 30, or 43 amino acids, including the M2e peptide (M122, M130, and M143 variants, respectively), was trans lationally fused with the N terminus of β glucuronidase in the pBI121 plant expression vector. The nucle otide sequence of the target fragment was synthesized by ligation from synthetic oligonucleotides; its codon composition was adapted for expression in plants. Tobacco plants were successfully transformed with the obtained vectors (pBIM122, pBIM130, and pBIM143, respectively). In the plants transformed with pBIM143, the Ml43-β glucuronidase fusion protein was not produced, probably due to the presence of the M2 protein transmembrane domain (25-43 aa of M2) in this construct. In the pBIM122 and pBIMl30 transformed plants, the target M2e peptide was expressed as a component of the Ml22-β glucuronidase and M130-β glucuronidase fusion proteins, respectively, as was detected by Western blot analysis. These proteins were detected as bands of the expected size without apparent degradation. As a result, the M2e peptide of the H5N1 avian influenza virus was successfully synthesized for the first time in nuclear transformed transgenic plants. The results obtained in this study will be used for developing a transgenic plant based edible anti influenza vaccine.

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mentioning

confidence: 75%

Expression of the nucleotide sequence for the M2e peptide of avian influenza virus in transgenic tobacco plants

Tarasenko

Taranov

Фирсов

et al. 2013

Appl Biochem Microbiol

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“…The stable transformation of crop species takes much more time, but represents the most scalable production method [9]-and potentially the most controversial from a social perspective. PMF represents the third 'generation' of genetically modified (GM) plants: first-generation crops were bred for productivity, second-generation crops for food quality and thirdgeneration crops for the production of PMPs and industrial purposes [28,29].…”

Section: Newcotiana and Pharma-factorymentioning

confidence: 99%

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products

et al. 2020

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Plant molecular farming (PMF) is a convenient and cost-effective way to produce high-value recombinant proteins that can be used in the production of a range of health products, from pharmaceutical therapeutics to cosmetic products. New plant breeding techniques (NPBTs) provide a means to enhance PMF systems more quickly and with greater precision than ever before. However, the feasibility, regulatory standing and social acceptability of both PMF and NPBTs are in question. This paper explores the perceptions of key stakeholders on two European Union (EU) Horizon 2020 programmes-Pharma-Factory and Newcotianatowards the barriers and facilitators of PMF and NPBTs in Europe. One-on-one qualitative interviews were undertaken with N = 20 individuals involved in one or both of the two projects at 16 institutions in seven countries (Belgium, France, Germany, Italy, Israel, Spain and the UK). The findings indicate that the current EU regulatory environment and the perception of the public towards biotechnology are seen as the main barriers to scaling-up PMF and NPBTs. Competition from existing systems and the lack of plant-specific regulations likewise present challenges for PMF developing beyond its current niche. However, respondents felt that the communication of the benefits and purpose of NPBT PMF could provide a platform for improving the social acceptance of genetic modification. The importance of the media in this process was highlighted. This article also uses the multi-level perspective to explore the ways in which NPBTs are being legitimated by interested parties and the systemic factors that have shaped and are continuing to shape the development of PMF in Europe. PLOS ONEPLOS ONE | https://doi.org/10.1371/journal.pone.The use of plants as platforms for the expression of recombinant proteins was first demonstrated in the late 1980s and early 1990s -primarily with the tobacco plant [23][24][25]. It would take over a decade for the first commercial product to be launched [8], but since that time a PLOS ONEBarriers and facilitators to scaling-up plant molecular farming PLOS ONE | https://doi.org/10.Barriers and facilitators to scaling-up plant molecular farming PLOS ONE | https://doi.org/10.

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“…In addition, the accumulation of endogenous sugars and simple sugar derivatives have been successfully increased in sugar-storing sink organs such as fruits, sugarcane culms and sugarbeet tubers, via plant metabolic engineering (Patrick et al 2013). Furthermore, metabolic engineering approaches resulted in increments if the levels of lysine in corn, folate in tomatoes and ferritin in lettuce (Yonekura-Sakakibara and Saito 2006). Despite the controversy associated with genetically modified plants, these examples prove that the nutrient content of crop plants can be improved by metabolic engineering.…”

Section: The Importance Of Metabolomics For Plant Engineeringmentioning

confidence: 99%

Crop metabolomics: from diagnostics to assisted breeding

et al. 2018

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Background Until recently, plant metabolomics have provided a deep understanding on the metabolic regulation in individual plants as experimental units. The application of these techniques to agricultural systems subjected to more complex interactions is a step towards the implementation of translational metabolomics in crop breeding. Aim of Review We present here a review paper discussing advances in the knowledge reached in the last years derived from the application of metabolomic techniques that evolved from biomarker discovery to improve crop yield and quality. Key Scientific Concepts of Review Translational metabolomics applied to crop breeding programs.

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Review: genetically modified plants for the promotion of human health

Cited by 61 publications

References 41 publications

Expression of the nucleotide sequence for the M2e peptide of avian influenza virus in transgenic tobacco plants

Expression of the nucleotide sequence for the M2e peptide of avian influenza virus in transgenic tobacco plants

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products

Crop metabolomics: from diagnostics to assisted breeding

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