Review: imaging technologies for flow cytometry

Han, Yuanyuan; Gu, Yi; Zhang, Alex Ce; Lo, Yu‐Hwa

doi:10.1039/c6lc01063f

Cited by 229 publications

(185 citation statements)

References 69 publications

Supporting

Mentioning

182

Contrasting

Unclassified

Order By: Relevance

“…Currently, one of the most powerful commercially available IFC technology is the Amnis ® ImageStreamX ® MarkII system (MilliporeSigma, Seattle, WA), paired with the Amnis ® IDEAS ® software package (31). The Amnis ® platform flows hydrodynamically focused cell suspensions through a flow cell where they are illuminated with a combination of bright-field LED sources and, optionally, coherent laser lines.…”

Section: Rbc-mistmentioning

confidence: 99%

Label‐Free Analysis of Red Blood Cell Storage Lesions Using Imaging Flow Cytometry

et al. 2019

View full text Add to dashboard Cite

Deleterious changes, collectively termed as storage lesions, alter the characteristics of red blood cell (RBC) morphology during in vitro storage. Due to gradual loss of cellular membrane, RBCs lose their original biconcave disk shape and begin a process of spherical deformation that is characterized by well-defined morphological criteria. At the spheroechinocyte stage, the structure of RBC is irreversibly damaged and lacks the elasticity necessary to efficiently deliver oxygen. Quantifying the prevalence of spheroechinocytes could provide an important morphological measure of the quality of stored blood products. Unlike the conventional RBC morphology characterization assay involving light microscopy, we introduce a label-free assay using imaging flow cytometry (IFC). The technique captures 100,000 images of a sample and calculates a relative measure of spheroechinocyte population in a fraction of the time required by the conventional method. A comparative method study, measuring a morphological index for 11 RCC units through storage, found that the two techniques measured similar trends with IFC reporting the metric at an average of 3.9% higher. We monitored 18 RCC units between Weeks 1 and 6 of storage and found that the spheroechinocyte population increased by an average of 26.2%. The large (3.5-64.1%) variation between the units' spheroechinocyte population percentage at Week 1 suggests a possible dependence of blood product quality on donor characteristics. Given our method's ability to rapidly monitor large samples and refine morphological characterization beyond conventional methods, we believe our technique offers good potential for studying the underlying relationships between RBC morphology and blood storage lesions.Key terms deformability; erythrocyte; imaging flow cytometry; red blood cell morphology; storage lesion CURRENTLY, blood banks and hospital laboratories are devoid of convenient, label-free techniques to measure the morphological distribution of red blood cells (RBCs) stored in vitro. Parameters such as membrane shape and deformability are critical for understanding an RBC's functional efficacy as an oxygen transporter (1). Microscopy-related assays, considered the gold standard for RBC morphology analysis, stain and smear samples prior to manual characterization. High-throughput assays that can measure RBCs within environments closely resembling in vivo conditions could obtain a more insightful assessment of the morphology distribution.RBCs are stored in vitro primarily to enhance the oxygen carrying capacity of vulnerable patients via transfusions (2). Blood banks extract RBCs from donated blood using customized separation techniques and suspend the cells in a preservative fluid to increase its shelf-life (3). During storage of this red cell concentrate (RCC), typically set at a maximum of 6 weeks, RBCs undergo a sequence of significant biochemical and biomechanical changes, which have been collectively termed hypothermic storage lesions (4,5). RBC storage lesions cause the formation of m...

show abstract

Section: Rbc-mistmentioning

confidence: 99%

Label‐Free Analysis of Red Blood Cell Storage Lesions Using Imaging Flow Cytometry

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The usage of dichlobenil and the problems associated with groundwater contamination by BAM have resulted in intensive research and on-site monitoring of these compounds. Chromatographic techniques and immunoassays are used for detecting the BAM [61][62][63]. Han et al [61] described a simple and inexpensive microfluidic biosensor based on AuNPs-labeled antibodies visualized by an office desktop flatbed scanner.…”

Section: Measurement Of Metabolitesmentioning

confidence: 99%

Nanomaterial Based Biosensors for Detection of Biomarkers of Exposure to OP Pesticides and Nerve Agents: A Review

et al. 2017

View full text Add to dashboard Cite

Organophosphorus (OP) pesticides are primarily used as insecticides and chemical warfare agents worldwide. Due to their impact on the environment and health, it is important to develop prompt and accurate pesticide analysis method. This review addresses recent advances and new trends in nanotechnology‐based biosensors for biological monitoring of exposures to OP pesticides and nerve agents. In order to determine them, we have to find the corresponding biomarkers. In 1989, the national academy of sciences (NAS)divided biomarkers into the following three categories: biomarker of exposure, biomarker of effect and biomarker of susceptibility (Figure 1A). The unique chemical and physical properties of nanomaterial have paved the way to new and improved sensing devices, in general, and electrochemical/optical biosensors, in particular. In this paper, background information and a general overview of electrochemical/immunoassay detection techniques are provided. Various nanomaterial labels are discussed. Usually nanomaterials can be roughly divided into nanometer powder, nanometer fiber, nanometer film, nanometer block and so on four classes, such as colloidal gold, semiconductor nanoparticles and carbon nanomaterial (Figure 1B). In addition, we discuss some future considerations and opportunities for advancing the use of biosensors for environmental and health studies.

show abstract

“…Based on these theoretical principles, the lens-less imaging or microscopy was later developed in the 1960s [27], and it has been evolving since then. Recently, mainly driven by technological developments in CMOS cameras, light sources and data processing resources, there has been an increasing number of applications and published works in the field of lens-less microscopy, but these have been mainly oriented to biological applications, focusing on the inspection of microorganisms and cells in biological samples [28].…”

Section: Introductionmentioning

confidence: 99%

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Mabe

Zubía

Gorritxategi³

2017

Sensors

View full text Add to dashboard Cite

The presence of microscopic particles in suspension in industrial fluids is often an early warning of latent or imminent failures in the equipment or processes where they are being used. This manuscript describes work undertaken to integrate different photonic principles with a micro- mechanical fluidic structure and an embedded processor to develop a fully autonomous wear debris sensor for in-line monitoring of industrial fluids. Lens-less microscopy, stroboscopic illumination, a CMOS imager and embedded machine vision technologies have been merged to develop a sensor solution that is able to detect and quantify the number and size of micrometric particles suspended in a continuous flow of a fluid. A laboratory test-bench has been arranged for setting up the configuration of the optical components targeting a static oil sample and then a sensor prototype has been developed for migrating the measurement principles to real conditions in terms of operating pressure and flow rate of the oil. Imaging performance is quantified using micro calibrated samples, as well as by measuring real used lubricated oils. Sampling a large fluid volume with a decent 2D spatial resolution, this photonic micro sensor offers a powerful tool at very low cost and compacted size for in-line wear debris monitoring.

show abstract

Review: imaging technologies for flow cytometry

Cited by 229 publications

References 69 publications

Label‐Free Analysis of Red Blood Cell Storage Lesions Using Imaging Flow Cytometry

Label‐Free Analysis of Red Blood Cell Storage Lesions Using Imaging Flow Cytometry

Nanomaterial Based Biosensors for Detection of Biomarkers of Exposure to OP Pesticides and Nerve Agents: A Review

Photonic Low Cost Micro-Sensor for in-Line Wear Particle Detection in Flowing Lube Oils

Contact Info

Product

Resources

About