Review: Mytilus galloprovincialis: An essential, low-cost model organism for the impact of xenobiotics on oxidative stress and public health

Curpăn, Alexandrina-Ștefania; Impellitteri, Federica; Plăvan, Gabriel; Ciobîcă, Alin; Faggio, Caterina

doi:10.1016/j.cbpc.2022.109302

Cited by 50 publications

(17 citation statements)

References 84 publications

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“…Therefore, the critical value of the application of natural antioxidants lies in their capacity to suppress oxidation processes, inhibit the free radical formation and/or interrupt propagation of autoxidation and reduce the levels of oxidative products in the animal and food system. Besides the diets, animal health status is also an important factor in the process of antioxidants’ deposition into the eggs [ 56 , 57 ]. It was previously reported that the antioxidative effects of rosehip were effective in defeating the reactive oxygen species due to substantial contributions from total polyphenols [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Rosehip (Rosa canina L.) Meal as a Natural Antioxidant on Lipid and Protein Quality and Shelf-Life of Polyunsaturated Fatty Acids Enriched Eggs

Vlaicu¹,

Untea²,

Turcu³

et al. 2022

Antioxidants

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Eggs are a common food of animal origin, inexpensive, and rich in bioactive substances with high biological value. Eggs enriched in polyunsaturated fatty acids (PUFA) are extremely desired by the progressive consumer. However, during storage, eggs undergo some physiochemical changes, which decrease their value. In this regard, the effect of dietary rosehip meal and flaxseed meal on hens’ egg quality characteristics, amino acids, fatty acids, health-related indices, antioxidant capacity, total polyphenols content, and shelf life was examined. For this study 120 Tetra SL laying hens, 29 weeks of age, were fed, for 4 weeks, three diets that included control (basal diet—RF0), basal diet + 1.5% rosehip and 7% flaxseed meal (RF1), and basal diet + 3% rosehip and 7% flaxseed meal (RF2). Productive performance of hens were recorded. The content of essential amino acids (EAA), antioxidant amino acids (AAA), and sulfur amino acids (SAA) was higher in RF1 and RF2, compared with RF0. Eggs belonging to the RF1 and RF2 groups had significantly (p < 0.05) higher content of n-3 PUFAs, especially linolenic and docosahexaenoic acids. Total antioxidant capacity and polyphenol content increased in both rosehip supplemented groups, but especially in RF2. Moreover, eggs from RF1 and RF2 groups maintained significantly higher egg quality parameters after storage for 14 and 28 days in the refrigerator (5 °C) and ambient temperature (21 °C), compared with those from the RF0 group. In the Haugh unit, yolk and albumen pH presented better values in RF1 and RF2 eggs compared to the RF0 eggs.

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Section: Discussionmentioning

confidence: 99%

Rosehip (Rosa canina L.) Meal as a Natural Antioxidant on Lipid and Protein Quality and Shelf-Life of Polyunsaturated Fatty Acids Enriched Eggs

Vlaicu¹,

Untea²,

Turcu³

et al. 2022

Antioxidants

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“…The digestive gland is a pivotal organ for M. galloprovincialis as it plays a crucial role in digestion and metabolism, facilitating the absorption of environmental compounds, including xenobiotics (Pagano et al, 2022; Tresnakova, Famulari, et al, 2023; Tresnakova, Impellitteri, et al, 2023). Any decline in the health of these cells can have significant implications for the overall well‐being of the organism (Curpan et al, 2022; Impellitteri, Multisanti, Rusanova, Piccione, et al, 2023; Impellitteri, Yunko, Martyniuk, Khoma, et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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“…A reason can be probably found in the open circulatory system, which allows haemocytes to reach more distant peripheral tissues (Matozzo, Battistara, et al, 2016). Similarly, the giant clam, Tridacna crocea , contains fewer haemocytes than the pearl oyster (from 0.3 to 2.6 ×10 6 cells ml −1 ), but also species that are similar in size to P. imbricata radiata , e.g., Mytilus edulis (5.68 × 10 6 cells ml −1 ) (Matozzo & Bailo, 2015), Mytilus galloprovincialis (5.16 × 10 6 cells ml −1 ) (Curpan et al, 2022; Munari et al, 2018), and Callista chione (1.2 × 10 6 cells ml −1 ) (Matozzo & Bailo, 2015), show fewer haemolymph cells.…”

Section: Discussionmentioning

confidence: 99%

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Pagano

Fabrello

Multisanti

et al. 2022

Microscopy Res & Technique

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The pearl oyster Pinctada imbricata radiata (Leach, 1814), from the Pacific Ocean, was one of the first species to reach via Suez the Mediterranean, colonizing the eastern basin and recently spreading to the western. The species showed to be able to adapt to a wide range of climatic, hydrological, and ecological conditions. Since 2000 it reached the Strait of Messina, where is now infesting the transitional waters of the oriented natural reserve “Laguna di Capo Peloro.” Due to such resistance and adaptation ability, various assays were performed. Haemocyte morpho‐functional aspects were evaluated in haemolymph samples fixed with 1% and 2% glutaraldehyde for optical and electron microscopy (TEM). The following assays were carried out: cell characterization using several dyes, detection of intra‐ and extracellular lipids, the capability of phagocytosis using the yeast Saccharomyces cerevisiae and to produce superoxide anion (O2−). Detection of several enzymes, such as acid and alkaline phosphatase, arylsulfatase, chloro‐acetylesterase and β‐glucuronidase was also assessed. Cell count was demonstrated to be abundant with a mean of 8.263 × 106 mm2 ± 0.935 × 106 (SD). Two main cell populations were noticed: granulocytes and hyalocytes, both competent for phagocytosis, to produce O2−, and characterized by lipids. Based on the granule analysis, enzymatic activity was also demonstrated. The observations under TEM confirmed all the results obtained. This study supports the hypothesis that P. imbricata radiata can be usefully employed as a model organism in environmental biomonitoring. Moreover, since the species represent potential threats to native species and ecosystems, further insights into its biological adaptations in invaded ecosystems are recommended.

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Review: Mytilus galloprovincialis: An essential, low-cost model organism for the impact of xenobiotics on oxidative stress and public health

Cited by 50 publications

References 84 publications

Rosehip (Rosa canina L.) Meal as a Natural Antioxidant on Lipid and Protein Quality and Shelf-Life of Polyunsaturated Fatty Acids Enriched Eggs

Rosehip (Rosa canina L.) Meal as a Natural Antioxidant on Lipid and Protein Quality and Shelf-Life of Polyunsaturated Fatty Acids Enriched Eggs

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

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