Review of Acellular and Cellular Artificial Skins

Suzuki, Shigehiko; Matsuda, Kazuya; Nishimura, Yoshihiko; Maruguchi, Yukiya; Maruguchi, Tomoko; Ikada, Yoshito; Morita, Sin-Ichiro; Morota, Katsuyasu

doi:10.1089/ten.1996.2.267

Cited by 24 publications

(6 citation statements)

References 23 publications

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“…In addition, unlike Integra®, the matrix in Pelnac® is not chemically cross-linked with glycosaminoglycan (GAG) and is still able to provide similar resistance to degradation against fibroblast collagenases. It allows a durable construct without the added cost; the cost of Pelnac® is US$4/cm 2 , while the cost of Integra® is US$8/cm 2 [7,16,17].…”

Section: Discussionmentioning

confidence: 99%

“…Integra® consists of a bilayer artificial dermis made up of bovine collagen and glycosaminoglycan matrix covered with an inert layer of silicone. It has the ability to facilitate organised infiltration of host's fibroblast and endothelial cells and regenerate a neodermis in 14-21 days. Pelnac® (Gunze Corp., Osaka, Japan) is another DRT which was first described in Japan by Suzuki et al, with the aim of expanding the indications and applications of DRTs [7,8]. It works on similar principles with several distinct properties with regards to composition; the details will be further discussed later.…”

Section: Introductionmentioning

confidence: 99%

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The use of dermal regeneration template (Pelnac®) in acute full-thickness wound closure: A case series

Widjaja

Maitz

2015

Eur J Plast Surg

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Background Dermal regeneration template (DRT) has been well implicated in the reconstruction of fullthickness burn injury. This case series specifically presents our experience and our clinical application of Pelnac® to achieve wound closure with complex acute full-thickness defect. Methods A retrospective review of patients treated with Pelnac for complex wound defects from 2008 to 2014 at Concord Burns Unit was carried out. Variables such as wound aetiology, wound size and complications were considered. Results Five patients (four females and one male with a mean age 54±20) all had full-thickness defects (mean defect size 4.3±2.0 % TBSA), some with exposed tendon and bone. The wounds were treated with Pelnac®; the silicone layer was removed at postoperative day 14 and a split-thickness skin graft (0.2 to 0.3 mm) was applied. Clinically, the reconstructed areas demonstrated good granulation tissue at 14 days with good take of the skin graft. There were no major acute graft loss, rejection or associated infection. However, there were small areas of graft loss which did not require regrafting.Conclusions DRT provides a safe and efficacious alternative when dealing with acute contaminated full-thickness wounds. Pelnac® seems versatile in obtaining wound coverage in difficult complex wounds, especially in critically ill patients where free or pedicle flap reconstruction would be problematic. Level of Evidence: Level V, therapeutic study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The use of dermal regeneration template (Pelnac®) in acute full-thickness wound closure: A case series

Widjaja

Maitz

2015

Eur J Plast Surg

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“…PELNAC™ (Gunze Ltd., Tokyo, Japan) is a commercially available full-thickness skin replacement scaffold. PELNAC™ is a freeze-dried crosslinked sponge layer (3 mm thickness) of porcine tendon-derived atelocollagen type I (dermal component) with a perforated silicone membrane (epidermal component) [10,11,12]. Atelocollagen is a low-immunogenic derivative of collagen obtained by removal of N- and C-terminal telopeptide components with type I pepsin [13].…”

Section: Methodsmentioning

confidence: 99%

Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies

Ballesteros-Cillero

Davison-Kotler

Kohli

et al. 2019

Cells

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Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds.

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“…Bilayered biomaterials composed of an inner porous collagen sheet, with or without chondroitin -6 -sulfate, and an outer silicone layer have been clinically applied to skin tissue engineering [12,13] . When placed on wounds even without any cell seeding, the collagen scaffold is replaced by a regenerated dermis -like tissue, while the silicone layer can be readily peeled off.…”

Section: Skinmentioning

confidence: 99%