Review of applications of fluorescence excitation spectroscopy to dermatology

Franco, Walfre; Gutierrez-Herrera, Enoch; Kollias, Nikiforos; Doukas, Apostolos G.

doi:10.1111/bjd.14221

Cited by 37 publications

(39 citation statements)

References 36 publications

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“…In our small sample, the 330 nm excitation band tended to increase with age, consistent with a progressive accumulation of pentosidine, which is in agreement with results reported by Handl et al [23], Uchiyama et al [39] and Senolt [41], in which the concentrations of pentosidine in serum and synovial fluids were observed to be elevated in patients with rheumatoid arthritis (RA) and osteoarthritis. It has also been shown in skin that the fluorescence intensity of this band changes with age [42]. Indentation stiffness E' showed a modest increase with age in our study, but this increase was not statistically significant [R = 0.77, (p = 0.07)].…”

Section: Discussioncontrasting

confidence: 61%

Intrinsic fluorescence and mechanical testing of articular cartilage in human patients with osteoarthritis

Padilla-Martínez

Lewis

Ortega-Martínez

et al. 2017

Journal of Biophotonics

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The degeneration of articular cartilage is the main cause of osteoarthritis (OA), a common cause of disability among elderly patients. The aim of this study is to understand the correlation between intrinsic fluorescence of articular cartilage and its biomechanical properties in patients with osteoarthritis. Cylindrical samples of articular cartilage 6 mm in diameter were extracted via biopsy punch from the femoral condyles of 6 patients with advanced OA undergoing knee replacement surgery. The mechanical stiffness and fluorescence of each cartilage plug were measured by indentation test and spectrofluorometry. Maps of fluorescence intensity, at excitation/emission wavelengths of 240-520/290-530 nm, were used to identify wavelengths of interest. The mechanical stiffness and fluorescence intensity were correlated using a Spearman analysis.

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Section: Discussioncontrasting

confidence: 61%

Intrinsic fluorescence and mechanical testing of articular cartilage in human patients with osteoarthritis

Padilla-Martínez

Lewis

Ortega-Martínez

et al. 2017

Journal of Biophotonics

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“…This emission band is most likely due to aromatic amino acids: tyrosine, tryptophan, and phenylalanine . As radiation with wavelengths shorter than 300 nm does not propagate significantly into the dermis, the sampled tissue volume for 280 nm radiation resides in the epidermis . Therefore, the observed peak at 340/280 nm (peak emission/peak excitation wavelength) should originate primarily from proteins located in the epidermis of human skin.…”

Section: Discussionmentioning

confidence: 99%

“…The 325 nm excitation generates skin autofluorescence in the region between 360 and 480 nm, apparently consisting of several partially or fully overlapping fluorescence bands with maxima at approximately 390, 420, and 450 nm. The 325 nm excitation can penetrate down to dermis . The most abundant dermis fluorophore is collagen, which, on excitation at 325 nm gives intensive emission peaked at 390‐400 nm with shoulders at around 420 and 460 nm .…”

Section: Discussionmentioning

confidence: 99%

“…Elastin is not as plentiful in the skin as collagen, so its skin autofluorescence may be hidden in the collagen emission band. The fluorescence of collagen and elastin cross‐links increases with age due to accumulation of AGEs . Since the AGEs comprise of several species, they can contribute to the whole 360‐450 nm emission band; however, our experiments were done on young healthy adults, for whom the contribution of AGEs may not be important yet.…”

Section: Discussionmentioning

confidence: 99%

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Gender differences in the fluorescence of human skin in young healthy adults

Morvová

Jeczko

Šikurová

2018

Skin Research and Technology

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The intensity of skin autofluorescence showed differences between genders and among anatomical regions. The 340 nm intensity was 1.4 times higher in the male group in all 3 anatomical regions studied. The highest intensity of skin autofluorescence for the peaks at 340/280 nm and 600/400 nm was found on the forehead, whereas the 390/325 nm band was most intensive on the inner upper arm in both genders.

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“…Our group previously developed a UV fluorescence imaging prototype capable of producing images of intrinsic UV fluorescence of tissue 1,2 . Several fluorescence excitation/emission pairs have been identified with molecules that are associated with the structure and function of the skin 3,4 .…”

Section: Introductionmentioning

confidence: 99%

Statistical image segmentation for the detection of skin lesion borders in UV fluorescence excitation

Ortega-Martínez¹,

Padilla-Martínez²,

Franco³

2016

SPIE Proceedings

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The skin contains several fluorescent molecules or fluorophores that serve as markers of structure, function and composition. UV fluorescence excitation photography is a simple and effective way to image specific intrinsic fluorophores, such as the one ascribed to tryptophan which emits at a wavelength of 345 nm upon excitation at 295 nm, and is a marker of cellular proliferation. Earlier, we built a clinical UV photography system to image cellular proliferation. In some samples, the naturally low intensity of the fluorescence can make it difficult to separate the fluorescence of cells in higher proliferation states from background fluorescence and other imaging artifacts --like electronic noise. In this work, we describe a statistical image segmentation method to separate the fluorescence of interest. Statistical image segmentation is based on image averaging, background subtraction and pixel statistics. This method allows to better quantify the intensity and surface distributions of fluorescence, which in turn simplify the detection of borders. Using this method we delineated the borders of highly-proliferative skin conditions and diseases, in particular, allergic contact dermatitis, psoriatic lesions and basal cell carcinoma. Segmented images clearly define lesion borders. UV fluorescence excitation photography along with statistical image segmentation may serve as a quick and simple diagnostic tool for clinicians.

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Review of applications of fluorescence excitation spectroscopy to dermatology

Cited by 37 publications

References 36 publications

Intrinsic fluorescence and mechanical testing of articular cartilage in human patients with osteoarthritis

Intrinsic fluorescence and mechanical testing of articular cartilage in human patients with osteoarthritis

Gender differences in the fluorescence of human skin in young healthy adults

Statistical image segmentation for the detection of skin lesion borders in UV fluorescence excitation

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