Review of Fluorescence Suppression Techniques in Raman Spectroscopy

Wei, Dong; Chen, Shuo; Liu, Quan

doi:10.1080/05704928.2014.999936

Cited by 251 publications

(167 citation statements)

References 108 publications

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“…[19] Thep rinciple behind these approaches is that the fluorescence emission does not significantly vary upon small wavelength shifts whereas the Raman bands shift as afunction of the excitation wavelength. [22]. [20] Dedicated lasers that emit two slightly shifted wavelengths were introduced by PD-LD (http://www.pd-ld.…”

Section: Abrief Introduction To the Theory Of Linear And Nonlinear Ramentioning

confidence: 99%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label-free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface-enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman-active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber-based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.

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Section: Abrief Introduction To the Theory Of Linear And Nonlinear Ramentioning

confidence: 99%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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“…This is due to the basic drawback of Raman spectroscopy, i.e., data treatment and confidence limits are often not really optimized, and a high fluorescence background causes high threshold values for most analytes in biological samples, and thus a low sensitivity . In addition, the fluorescence background often dominates the Raman spectrum even when using common near‐infrared (NIR) laser excitation sources …”

Section: Introductionmentioning

confidence: 99%

Comparison of time‐gated surface‐enhanced raman spectroscopy (TG‐SERS) and classical SERS based monitoring of Escherichia coli cultivation samples

Kögler

Paul

Anane

et al. 2018

Biotechnology Progress

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The application of Raman spectroscopy as a monitoring technique for bioprocesses is severely limited by a large background signal originating from fluorescing compounds in the culture media. Here, we compare time-gated Raman (TG-Raman)-, continuous wave NIR-process Raman (NIR-Raman), and continuous wave micro-Raman (micro-Raman) approaches in combination with surface enhanced Raman spectroscopy (SERS) for their potential to overcome this limit. For that purpose, we monitored metabolite concentrations of Escherichia coli bioreactor cultivations in cell-free supernatant samples. We investigated concentration transients of glucose, acetate, AMP, and cAMP at alternating substrate availability, from deficiency to excess. Raman and SERS signals were compared to off-line metabolite analysis of carbohydrates, carboxylic acids, and nucleotides. Results demonstrate that SERS, in almost all cases, led to a higher number of identifiable signals and better resolved spectra. Spectra derived from the TG-Raman were comparable to those of micro-Raman resulting in well-discernable Raman peaks, which allowed for the identification of a higher number of compounds. In contrast, NIR-Raman provided a superior performance for the quantitative evaluation of analytes, both with and without SERS nanoparticles when using multivariate data analysis. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., :1-10, 2018.

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“…In the context of fuel characterization, aromatic compounds are likely to emit fluorescence, when they are excited in the ultraviolet or visible spectral range. An overview of techniques to suppress fluorescence interference in Raman spectra can be found in reference [23].…”

Section: Fundamentalsmentioning

confidence: 99%

“…In order to avoid such effects a number of approaches have been developed including near-infrared Raman [26], deep-UV Raman [27], polarization-resolved detection Raman [28], and shifted-excitation Raman difference spectroscopy (SERDS) [29][30][31][32]. An overview can be found in reference [23].  The 90°-setup normally requires three optical accesses, which cannot always be arranged in a technical object.…”

Section: Advantages and Disadvantagesmentioning

confidence: 99%

Recent Advances in the Characterization of Gaseous and Liquid Fuels by Vibrational Spectroscopy

Kiefer

2015

Energies

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Most commercial gaseous and liquid fuels are mixtures of multiple chemical compounds. In recent years, these mixtures became even more complicated when the suppliers started to admix biofuels into the petrochemical basic fuels. As the properties of such mixtures can vary with composition, there is a need for reliable analytical technologies in order to ensure stable operation of devices such as internal combustion engines and gas turbines. Vibrational spectroscopic methods have proved their suitability for fuel characterization. Moreover, they have the potential to overcome existing limitations of established technologies, because they are fast and accurate, and they do not require sampling; hence they can be deployed as inline sensors. This article reviews the recent advances of vibrational spectroscopy in terms of infrared absorption (IR) and Raman spectroscopy in the context of fuel characterization. The focus of the paper lies on gaseous and liquid fuels, which are dominant in the transportation sector and in the distributed generation of power. On top of an introduction to the physical principles and review of the literature, the techniques are critically discussed and compared with each other.

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Review of Fluorescence Suppression Techniques in Raman Spectroscopy

Abstract: It incorporates referee's comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The

Cited by 251 publications

References 108 publications

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Comparison of time‐gated surface‐enhanced raman spectroscopy (TG‐SERS) and classical SERS based monitoring of Escherichia coli cultivation samples

Recent Advances in the Characterization of Gaseous and Liquid Fuels by Vibrational Spectroscopy

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