Review of Hair Follicle Dermal Papilla cells as<i>in vitro</i>screening model for hair growth

Madaan, Alka; Verma, Ritu; Singh, Anu; Jaggi, Manu

doi:10.1111/ics.12489

Cited by 125 publications

(87 citation statements)

References 204 publications

(200 reference statements)

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“…Hair disorders such as hair loss (alopecia), androgenic alopecia, or excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual [1,2]. Various agents, including herbal and natural extracts [3][4][5], growth factors, cytokines [6,7], placental extract, stem cells [8], conditioned medium, peptides, hormones, lipid nanocarrier, light, physical stimulation [9], androgens and their analogs, stress-serum, and chemotherapeutic agents, have been examined for their hair-growth-modulating effects in hDPCs.…”

Section: Introductionmentioning

confidence: 99%

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

Kim

Lim

et al. 2020

IJMS

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Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia.

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Section: Introductionmentioning

confidence: 99%

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

Kim

Lim

et al. 2020

IJMS

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“…However, although many papers on PRP have been published, the results are often contradictory. For HF-derived research [52][53][54], studies mainly include hair follicle stem cells (HFSCs) and dermal papilla cells (DPCs). Currently, treatment using HFSCs alone is progressing to clinical trials through preclinical models.…”

Section: Discussionmentioning

confidence: 99%

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Dai¹,

Fei²,

Xu³

et al. 2020

IJMS

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Increasing cashmere yield is one of the important goals of cashmere goat breeding. To achieve this goal, we screened the key genes that can improve cashmere performance. In this study, we used the RNA raw datasets of the skin and dermal papilla cells of secondary hair follicle (SHF-DPCs) samples of hair follicle (HF) anagen and telogen of Albas cashmere goats and identified a set of significant differentially expressed genes (DEGs). To explore potential associations between gene sets and SHF growth features and to identify candidate genes, we detected functional enrichment and constructed protein-protein interaction (PPI) networks. Through comprehensive analysis, we selected Thymosin β4 (Tβ4), Rho GTPase activating protein 6 (ARHGAP6), ADAM metallopeptidase with thrombospondin type 1 motif 15, (ADAMTS15), Chordin (CHRD), and SPARC (Osteonectin), cwcv and kazal-like domains proteoglycan 1 (SPOCK1) as candidate genes. Gene set enrichment analysis (GSEA) for these genes revealed Tβ4 and ARHGAP6 have a close association with the growth and development of SHF-DPCs. However, the expression of Tβ4 in the anagen was higher than that in the telogen, so we finally chose Tβ4 as the ultimate research object. Overexpressing Tβ4 promoted and silencing Tβ4 inhibited the proliferation of SHF-DPCs. These findings suggest that Tβ4 can promote the growth and development of SHF-DPCs and indicate that this molecule may be a valuable target for increasing cashmere production.

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“…Follicular keratinocytes are in fact implicated in the exclusive expression of keratins constituting the hair structure (K81-K86 (type II), K31-K40 (type I)), located both in the cortex and in the cuticle, representing the most important cells within the hair follicle involved in hair growth [11][12][13]. Dermal cells are specialized mesenchymal cells located at the base of the hair follicle that play an essential role in hair follicular morphogenesis and in all regulation processes controlling postnatal hair growth cycles [42]. Overall, by AMS treatment in vitro we found a remarkable increase of hair keratins expression in keratinocytes, as well as of cytokeratins in dermal papilla cells, already at concentrations of 0.5 and 1 mg/mL of the nutraceutical formulation.…”

Section: Discussionmentioning

confidence: 99%

Induction of Hair Keratins Expression by an Annurca Apple-Based Nutraceutical Formulation in Human Follicular Cells

et al. 2019

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Hair disorders may considerably impact the social and psychological well-being of an individual. Recent advances in the understanding the biology of hair have encouraged the research and development of novel and safer natural hair growth agents. In this context, we have previously demonstrated-at both preclinical and clinical level-that an Annurca apple-based dietary supplement (AMS), acting as a nutraceutical, is endowed with an intense hair-inductive activity (trichogenicity), at once increasing hair tropism and keratin content. Herein, in the framework of preclinical investigations, new experiments in primary human models of follicular keratinocytes and dermal papilla cells have been performed to give an insight around AMS biological effects on specific hair keratins expression. As well as confirming the biocompatibility and the antioxidant proprieties of our nutraceutical formulation, we have proven an engagement of trichokeratins production underlying its biological effects on human follicular cells. Annurca apples are particularly rich in oligomeric procyanidins, natural polyphenols belonging to the broader class of bioflavonoids believed to exert many beneficial health effects. To our knowledge, none of the current available remedies for hair loss has hitherto shown to stimulate the production of hair keratins so clearly.

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Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Cited by 125 publications

References 204 publications

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

Thymosin β4 Identified by Transcriptomic Analysis from HF Anagen to Telogen Promotes Proliferation of SHF-DPCs in Albas Cashmere Goat

Induction of Hair Keratins Expression by an Annurca Apple-Based Nutraceutical Formulation in Human Follicular Cells

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