Review of Research in Developing Hydrogels with Insulin to Promote Wound Healing

Ostróżka-Cieślik, Aneta; Przybyła, Marcin; Wójcik, Weronika; Birówka, Klaudia; Majczyna, Marta; Dolińska, Barbara

doi:10.3390/ecb2023-14290

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…At the beginning of the 20th century, there was a growing interest in insulin as a potential active substance to accelerate the regeneration of damaged skin. In our previous article, we reviewed studies published between 2000 and 2022 on the therapeutic efficacy of a hydrogel form of insulin for skin application [1]. The analysis confirms the efficacy of this hormone in the treatment of hard-to-heal wounds without causing side effects such as hypoglycemia.…”

Section: Introductionmentioning

confidence: 86%

“…The topical application of insulin can reverse the impairment of diabetic wound regeneration processes and promote wound healing. The therapeutic effect of insulin after topical administration is mainly due to its role in regulating oxidative stress and inflammatory responses [1][2][3][4]. Chen et al [5] found that insulin is involved in the early recruitment of neutrophils, increase in F2 macrophages and IL-10 levels [6].…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge, there is no topical insulin application formulation on the market. A number of pharmaceutical forms of insulin for topical application are undergoing preclinical and clinical trials [1]. Research into the development of hydrogel forms of insulin seem particularly promising.…”

Section: Introductionmentioning

confidence: 99%

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Hydrogel Formulations for Topical Insulin Application: Preparation, Characterization and In Vitro Permeation across the Strat-M® Membrane

Ostróżka-Cieślik,

Wilczyński,

Dolińska

2023

Polymers

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Insulin has shown efficacy in the treatment of hard-to-heal wounds, which is mainly due to its role in regulating oxidative stress and inflammatory reactions. The aim of this study was to develop an insulin–hydrogel carrier based on Sepineo™ P 600 and Sepineo™ PHD 100 for application to lesional skin. Preformulation studies of the developed formulations were performed in terms of analysis of the pharmaceutical availability of insulin from the hydrogels through the Strat-M® membrane, and rheological and texture measurements. Insulin is released in a prolonged manner; after a time of 6.5 h, 4.01 IU/cm2 (53.36%) and 3.69 IU/cm2 (47.4%) of the hormone were released from the hydrogel based on Sepineo™ P 600 and Sepineo™ PHD 100, respectively. Rheological analysis showed that the hydrogels tested belong to non-Newtonian, shear-thinning systems with yield stress. The insulin–hydrogel based on Sepineo™ P 600 and Sepineo™ PHD 100 shows optimal application properties. The results obtained provide a basis for further preclinical and clinical studies.

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Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Hydrogel Formulations for Topical Insulin Application: Preparation, Characterization and In Vitro Permeation across the Strat-M® Membrane

Ostróżka-Cieślik,

Wilczyński,

Dolińska

2023

Polymers

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“…Over the past few years, this hormone has also been known for its skin regenerative properties. In addition to its great wound-healing effects, insulin is one of the cheapest growth factors to obtain biotechnologically, which is of significant value when it comes to producing dressings with this hormone on a large scale [1,2]. It has been confirmed that topical administration of insulin stimulates keratinocyte proliferation and migration in the wound tissue, depending on the dose and exposure time of the peptide.…”

Section: Introductionmentioning

confidence: 99%

Research Progress on Insulin Dressings to Promote Wound Healing

Przybyła,

Dolińska,

Ostróżka-Cieślik

2023

Asec 2023

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Healing of chronic wounds at the remodeling stage: the ratio of hormonal and immune parameters

Pavlov,

Babenko,

Kumetchko

et al. 2024

ЕМ

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Background. Both patients and healthcare systems around the world experience the negative consequences of chronic wounds. Chronic wounds often precede serious events such as amputation and premature death. Objective: to study the relationship between endocrine factors (insulin and cortisol) and bioactive molecules (interferon-γ (IFN-γ) and transforming growth factor-β1 (TGF-β1)), influencing the development of reparative processes of chronic wounds at the remodeling stage in an experiment, and to analyze the features of the histostructure of rat skin in the area of chronic wound healing. Materials and methods. The study was conducted on 12 white rats. Animals were randomized into intact and experimental groups, with 6 participants in each group. Chronic wounds were induced in the experimental group. Rats were euthanized on the 28th day of the experiment. In the blood serum, the insulin, cortisol, IFN-γ, and TGF-β1 levels were determined by enzyme immunoassay. Histological examination was carried out using generally accepted methods. Results. It was shown that the concentrations of insulin, cortisol, and TGF-β1 in animals of the experimental group were almost doubled compared to intact rats. The level of IFN-γ in animals with wounds was 1.2 times lower than in intact rats. Microscopic examination showed that the wounds were at the stage of remodeling. At the same time, signs of inflammation are partially preserved, which may indicate chronicity of the reparative process. Conclusions. Understanding the mechanisms of reparative processes during wound healing will allow for the development of clinical protocols to improve care for patients with injuries.

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Review of Research in Developing Hydrogels with Insulin to Promote Wound Healing

Abstract: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Cited by 4 publications

References 34 publications

Hydrogel Formulations for Topical Insulin Application: Preparation, Characterization and In Vitro Permeation across the Strat-M® Membrane

Hydrogel Formulations for Topical Insulin Application: Preparation, Characterization and In Vitro Permeation across the Strat-M® Membrane

Research Progress on Insulin Dressings to Promote Wound Healing

Healing of chronic wounds at the remodeling stage: the ratio of hormonal and immune parameters

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