In recent years, the importance of nanoparticle synthesis and application has increased, and as a result, more research has been conducted on nanoscience and nanotechnology. In particular, research on the production of environmentally friendly, less wasteful, low-cost, and multifunctional nanomaterials has increased dramatically. Some studies have been conducted on natural organic waste materials such as coconut husk, nutmeg seed, mint leaves, coffee beans, and tea leaves for producing nanostructures. Using organic waste materials as a source of nanostructures helps us save on disposal costs and helps the environment. Many different types of nanomaterials can be synthesized from organic waste materials such as, metallic nanoparticles, quantum dots, carbon-based nanomaterials, etc. Graphene quantum dots (G-QDs) are nanosized, two-dimensional particles, with sizes less than 100 nm that have amazing properties like being biocompatible, non-toxic, highly soluble, adjustable light emission, and photo-induced electron transfers. There are two main methods: top-down and bottom-up, which are used for the production of quantum dots. The microwave-assisted method is frequently used. Herein, we have envisaged synthesizing and characterizing G-QDs from organic waste materials, such as waste coffee grounds and tea leaves. Briefly, coffee beans and tea leaves were dried, ground, weighted, and extracted. A certain amount of coffee extract and tea leaf extract were used as precursors for the synthesis of G-QDs under the different conditions. The purification of G-QDs in this experiment was carried out by a high-speed (9000 rpm) centrifuge method. The synthesized G-QDs were characterized by UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Zeta potential. The characterization results indicate that the highly photoluminescent G-QDs were successfully manufactured. So, the structures of the final products were verified using spectroscopic methods such as UV-VIS, FT-IR, and DLS. This research focuses on creating high-quality G-QDs from biomass for practical use in sensing, photocatalysis, and biomedical applications.