The Nearctic fauna (north of Mexico) of Eustrophinae is reviewed, and consists of the following five genera and 12 species: Pseudoholostrophus (Pseudoholostrophus) impressicollis (LeConte), Pseudoholostrophus (Holostrophinus) discolor (Horn), Holostrophus bifasciatus (Say), Eustrophus tomentosus Say, Eustrophopsis confinis (LeConte), Eustrophopsis bicolor (Fabricius), Eustrophopsis brunneimarginatus (Dury), Eustrophopsis indistinctus (LeConte), Eustrophopsis arizonensis (Horn), Eustrophopsis ornatus (Van Dyke), Eustrophopsis crowdyi
sp. n., and Synstrophus repandus (Horn). A lectotype is designated for Eustrophus brunneimarginatus Dury. A key is given to separate genera and species, supplemented with illustrations of relevant features, including aedeagi of all Nearctic species of Eustrophopsis. Detailed distribution (including Mexican records) and natural history data are provided.