-The objective of this study was to examine the effect of the level of reducing nitrogen (N) fertiliser rate on herbage intake and N balance of dairy cows grazing a pure perennial ryegrass pasture. The addition of a protein supplement to cows grazing the low N fertilised sward was also evaluated. Three treatments were compared over three periods of 2 weeks using 9 fistulated cows in a 3 × 3 Latin square design: HN (80 kg N·ha -1 ·regrowth -1 ), LN (0-20 kg N·ha -1 ·regrowth -1 ), LN+S (LN + 2 kg of soybean meal (SBM)). Daily herbage organic matter (OM) intake was estimated by chromic oxide dilution in the faeces. Nitrogen and ADF contents in faecal OM were used to estimate the herbage digestibility. Herbage mass, grazing behaviour and rumen fermentation pattern were measured. Nitrogen intake was estimated by the chemical composition of the defoliated herbage and urinary N was calculated by subtracting milk N and faecal N output from N intake. Digestibility (0.79), daily intake (16.4 kg OM), grazing time (512 min) and the proportion of volatile fatty acid were not affected by reducing the N fertiliser rate. These results may be explained by the moderate effect of N fertilisation on herbage mass which remained high in the LN swards (3.9 vs. 4.7 t OM·ha -1 for HN) and the rather large herbage allowance which allowed the cows to graze a herbage with a crude protein content that still remained higher than 160 g·kg -1 DM. On the LN sward, N intake was significantly lower (-80 g·d -1 ) (P < 0.01), faecal N and milk N output remained unchanged, whereas urine N output decreased (-77 g·d -1 ) (P < 0.01). Protein supplementation did not depress HOMI or grazing time, supplemented cows consumed 2.4 kg OM more (P < 0.01) and this increased milk yield by 1.3 kg·kg -1 SBM (P < 0.01). SBM supplementation largely increased N intake, and finally N excreted in the urine. It was concluded that N fertilisation, and N supplementation are efficient means to manipulate animal performances and N balance in grazing dairy cows. . Res. 51 (2002) 279-293 INRA, EDP Sciences, 2002 DOI: 10.1051/animres:2002022Résumé -Influence du niveau de fertilisation azotée et de la complémentation azotée sur l'ingestion et le bilan azoté des vaches laitières pâturant du ray-grass anglais. L'effet du niveau de fertilisation azotée et de la complémentation avec du tourteau de soja (50 % normal et 50 % tanné) a été étudié chez des vaches laitières pâturant un ray-grass anglais. Trois traitements ont été comparés avec 9 vaches fistulées au niveau du rumen, selon un schéma en carré latin 3 × 3 sur des périodes de 2 semaines : HN (80 kg N·ha -1 ·cycle -1 ), LN (0-20 kg N·ha -1 ·cycle -1 ), LN+S (LN + 2 kg de tourteau de soja (SBM)). Les quantités d'herbe ingérée ont été calculées en mesurant la quantité de fèces par dilution de l'oxyde de chrome et en estimant la digestibilité de l'herbe ingérée à partir de la teneur en N et en ADF des fèces. L'azote ingéré a été estimé à partir de la composition chimique de l'herbe dé-foliée et l'azote urinaire a été ...