Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov.

Green, Peter N.; Ardley, Julie

doi:10.1099/ijsem.0.002856

Cited by 225 publications

(154 citation statements)

References 97 publications

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“…Furthermore, the fact that not all these studies used negative controls to identify potential contaminating OTUs and remove them from their datasets calls for caution in attempts to draw conclusions about these differences, particularly when we know that these OTUs can represent more than 50% of sequences detected in tick samples [22]. [6,12,16,18,19], were identified as bacteria present in the environment [57][58][59][60][61][62]. While very few of the above mentioned references reported performing negative controls while studying tick microbiota [12,19], it is important to keep in mind that some of these genera could also correspond to contaminants that may arise during extraction or amplification steps [22,30,[63][64][65].…”

Section: • Ixodes Ricinus Microbiota Diversity and Compositionmentioning

confidence: 99%

Temporal patterns inIxodes ricinusmicrobial communities: an insight into tick-borne microbe interactions

Lejal

Chiquet

Aubert

et al. 2020

Preprint

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BackgroundTicks transmit pathogens of medical and veterinary importance, and represent an increasing threat for human and animal health. Important steps in assessing disease risk and developing possible new future control strategies involve identifying tick-borne microbes, their temporal dynamics and interactions.MethodsUsing high throughput sequencing, we studied the microbiota dynamics of Ixodes ricinus from 371 nymphs collected monthly over three consecutive years in a peri-urban forest. After adjusting a Poisson Log Normal model to our data set, the implementation of a principal component analysis as well as sparse network reconstruction and differential analysis allowed us to assess inter-annual, seasonal and monthly variability of I. ricinus microbial communities as well as their interactions.ResultsAround 75% of the detected sequences belonged to five genera known to be maternally inherited bacteria in arthropods and potentially circulating in ticks: Candidatus Midichloria, Rickettsia, Spiroplasma, Arsenophonus and Wolbachia. The structure of the I. ricinus microbiota was temporally variable with interannual recurrence and seemed to be mainly driven by OTUs belonging to environmental genera. The total network analysis revealed a majority of positive (partial) correlations. We identified strong relationships between OTUs belonging to Wolbachia and Arsenophonus, betraying the presence of the parasitoid wasp Ixodiphagus hookeri in ticks, and the well known arthropod symbiont Spiroplasma, previously documented to be involved in the defense against parasitoid wasp in Drosophila melanogaster. Other associations were observed between the tick symbiont Candidatus Midichloria and pathogens belonging to Rickettsia, probably Rickettsia helvetica. More specific network analysis finally suggested that the presence of pathogens belonging to genera Borrelia, Anaplasma and Rickettsia might disrupt microbial interactions in I. ricinus.ConclusionsHere, we identified the I. ricinus microbiota and documented for the first time the existence and recurrence of marked temporal shifts in the tick microbial community dynamics. We statistically showed strong relationships between the presence of some pathogens and the structure of the I. ricinus non-pathogenic microbes. We interestingly detected close links between some tick symbionts and the potential presence of either pathogenic Rickettsia or a parasitoid in ticks. All these new findings might be very promising for the future development of new control strategies of ticks and tick-borne diseases.

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Section: • Ixodes Ricinus Microbiota Diversity and Compositionmentioning

confidence: 99%

Temporal patterns inIxodes ricinusmicrobial communities: an insight into tick-borne microbe interactions

Lejal

Chiquet

Aubert

et al. 2020

Preprint

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“…to grow on methanol is thought to be one of the reasons why these bacteria can dominate on leaf surfaces. In previous studies, the relationship between the ability to grow on methanol and the ability to colonize plant leaf surfaces was investigated using the representative model strain Methylobacterium extorquens AM1, which was originally isolated as an airborne contaminant growing on methylamine [15] and has recently been re-classified as Methylorubrum extorquens [16].…”

Section: Introductionmentioning

confidence: 99%

Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants

Yoshida

Iguchi

Sakai

et al. 2019

Bioscience, Biotechnology, and Biochemistry

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A number of pink-pigmented facultative methylotrophs (PPFMs) belonging to Methylobacterium spp. isolated from living plant samples were found to require B vitamins for their growth in minimal medium, and most B vitamin-auxotrophic PPFMs required pantothenate (vitamin B 5). Further investigation of pantothenate auxotrophy using the representative strain Methylobacterium sp. OR01 demonstrated that this strain cannot synthesize β-alanine, one of the precursors of pantothenate. β-alanine and several precursors of pantothenate restored the growth of Methylobacterium sp. OR01 in minimal medium. Furthermore, this strain could colonize leaves of Arabidopsis thaliana cultivated in medium without pantothenate or its precursors. Pantothenate, β-alanine and several precursors were detected in the suspension of A. thaliana leaves. These results suggest that pantothenate-auxotrophic PPFMs can symbiotically colonize the surface of plant leaves by acquiring β-alanine and other precursors, in addition to pantothenate. Finally, the fitness advantage of B vitamin auxotrophy of PPFMs in the phyllosphere environment is discussed.

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“…e genus has over fifty published species; however, based on the 16S rRNA gene, multilocus sequence analysis, and genomic and phenotypic data, fifty-two Methylobacterium species can no longer be regarded in this genus. A new genus, Methylorubrum gen. nov. has been proposed to accommodate 11 species, which were previously held in the Methylobacterium genus [35]. Additionally, this isolate is in a Methylobacterium clade that is sister to Methylorubrum gen nov clade, as demonstrated in Figure 1(a).…”

Section: Resultsmentioning

confidence: 80%

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Photolo

Mavumengwana

Sitole

et al. 2020

International Journal of Microbiology

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is study reports on the isolation and identification of Methylobacterium radiotolerans MAMP 4754 from the seeds of the medicinal plant, Combretum erythrophyllum, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte. e strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM). Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. e MICs were recorded at 250 μg/mL for B. subtilis ATCC 19659, B. cereus ATCC 1076, E. coli ATCC1053, and 62.5 μg/mL for K. oxytoca ATCC 13182 and M. smegmatis ATCC 21293, while an IC 50 of 5.65 μg/mL was recorded with the DPPH assay. Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids. Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities. M. radiotolerans MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain. with only 3% of these being antimicrobial [4]. e pharmaceutical properties of medicinal plants have been linked to the production of a wide variety of structurally diverse phytochemicals which include alkaloids, flavonoids, terpenes, steroids, curcumins, saponins, and phenolics, all of which can potentially serve as drug lead candidates for the development of antimicrobials and resistance modifiers [5,6].According to the International Union for Conservation of Nature and the World Wildlife, there are an estimated 80 000 recognized medicinal flowering plant species globally,

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Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov.

Cited by 225 publications

References 97 publications

Temporal patterns inIxodes ricinusmicrobial communities: an insight into tick-borne microbe interactions

Temporal patterns inIxodes ricinusmicrobial communities: an insight into tick-borne microbe interactions

Pantothenate auxotrophy of Methylobacterium spp. isolated from living plants

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

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