Review of the structural characterization, quality evaluation, and industrial application of Lycium barbarum polysaccharides

Wu, Ding‐Tao; Guo, Hongliang; Lin, Shang; Lam, Shing Chung; Zhao, Li; Lin, Derong; Qin, Wen

doi:10.1016/j.tifs.2018.07.016

Cited by 87 publications

(47 citation statements)

References 87 publications

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“…The contents of polyphenolics in PPP-W and PPP-P were remarkably higher than those in other tested PPPs, which might be correlated with this fact that polyphenolics can be associated with polysaccharides and/or proteins more easily because of high temperature and high pressure during HWE and PWE extraction [32], as well as the degradation of PPPs during ultrasoundand microwave-assisted extraction [15]. Extraction methods can influence molecular weights, viscosities, and monosaccharide compositions of polysaccharides, which can also affect the bioactivities of polysaccharides [17,33]. Accordingly, M w , viscosities, and monosaccharide compositions of PPPs extracted by seven extraction methods were studied.…”

Section: Discussionmentioning

confidence: 92%

“…The polydispersities of fractions 1 to 3 in PPP-U, PPP-UE, PPP-UM varied from 1.155 to 1.161, from 1.076 to 1.349, and from 1.104 to 1.154, respectively, which also matched with the HPSEC-RID chromatograms. Extraction methods can influence molecular weights, viscosities, and monosaccharide compositions of polysaccharides, which can also affect the bioactivities of polysaccharides [17,33]. Accordingly, Mw, viscosities, and monosaccharide compositions of PPPs extracted by seven extraction methods were studied.…”

Section: Discussionmentioning

confidence: 99%

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Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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“…Generally, the bioactivities of natural polysaccharides are associated with their molecular weights and constituent monosaccharides [23]. Figure 4A showed the HPSEC-RID chromatograms of KPS-M, KPS-U, and KPS-H. Two polysaccharide fractions (Figure 4A, fraction 1 and 2) were detected in KPS-M, KPS-U, and KPS-H.…”

Section: Resultsmentioning

confidence: 99%

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Han

Liu

et al. 2019

Molecules

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In order to evaluate effects of extraction techniques on the physicochemical characteristics and antioxidant activities of kiwifruit polysaccharides (KPS), and further explore KPS as functional food ingredients, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were optimized for the extraction of KPS. Furthermore, the physicochemical structures and antioxidant activities of KPS extracted by different techniques were investigated. The optimal extraction conditions of UAE and MAE for the extraction of KPS were obtained by response surface methodology. Different extraction techniques significantly affected the contents of uronic acids, molecular weights, molar ratios of constituent monosaccharides, and the degree of esterification of KPS. Results showed that KPS exhibited remarkable DPPH and ABTS radical scavenging activities, and reducing power. The high antioxidant activities observed in KPS extracted by the MAE method (KPS-M) might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acid. Results suggested that the MAE method could be a good potential technique for the extraction of KPS with high antioxidant activity, and KPS could be further explored as functional food ingredients.

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“…The molecular weight of natural resource polysaccharides has an important effect on its biological activity [28]. The molecular weight distribution of LBP, LBP < 10 kDa and LBP > 10 kDa was determined by high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID), which is a technique for independent determination of the absolute molecular weights of polymers in solution by detecting the scattered light of polymer components [29]. The HPSEC chromatogram of LBP showed two overlapping peaks at elution times of 16.17 min and 19.02 min ( Figure S2), with weight average molecular weight (Mw) of 51.88 kDa and 6.71 kDa, respectively.…”

Section: Preparation and Characterization Of Lbpmentioning

confidence: 99%

Immunomodulatory Effects of Lycium barbarum Polysaccharide Extract and Its Uptake Behaviors at the Cellular Level

et al. 2020

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Lycium barbarum L. is a widely used functional food and medicinal herb in Asian countries. L. barbarium polysaccharides (LBP) are considered as one of the major medicinal components of L. barbarium fruit and exhibits a wide range of biological activities. Here, we investigated the immunomodulatory effects of LBP and its uptake behaviors at the cellular level. LBP was prepared by water extraction and ethanol precipitation, and divided into two fractions based on the molecular weight distribution by ultrafiltration (LBP > 10 kDa and LBP < 10 kDa). The physicochemical properties of LBP and LBP fractions were well characterized. The LBP > 10 kDa fraction greatly enhanced the viability of macrophages RAW264.7 cells and induced cell polarization, but had weak effects to other tested tumor cell lines and normal cell line. This fraction could regulate the production of NO, TNF-α, IL-6 and ROS in RAW264.7 cells, suggesting both pro-inflammatory and anti-inflammatory effects. The dye-labeled LBP could be internalized into all tested cell lines and accumulated in lysosomes. The internalization of LBP in RAW264.7 cells is mainly through the clathrin-mediated endocytosis pathway. The Caco-2 intestinal transport experiment demonstrated that the dye labeled LBP could be transported through the Caco-2 cell monolayer (mimic intestinal epithelium) through clathrin-mediated endocytosis. These results demonstrate the immunomodulatory effects of LBP and its effective uptake by macrophages and intestine.

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Review of the structural characterization, quality evaluation, and industrial application of Lycium barbarum polysaccharides

Cited by 87 publications

References 87 publications

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Immunomodulatory Effects of Lycium barbarum Polysaccharide Extract and Its Uptake Behaviors at the Cellular Level

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