Review on bacterial production of alkaline pectinase with special emphasis on Bacillus species

Kavuthodi, Bijesh; Sebastian, Denoj

doi:10.21786/bbrc/11.1/4

Cited by 59 publications

(28 citation statements)

References 83 publications

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“…The chief source of acidic Pels is fungi, but alkaline Pels are produced from alkalophilic bacteria, primarily Bacillus spp. (Kavuthodi and Sebastian, 2018). These enzymes showed good performance in bioscouring and degumming applications.…”

Section: Introductionmentioning

confidence: 98%

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Zheng

Zhang

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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Pectate lyases play an essential role in textiles, animal feed, and oil extraction industries. Pichia pastoris can be an ideal platform for pectate lyases production, and BspPel (a thermo-alkaline pectate lyase from Bacillus sp. RN1) was overexpressed by combined strategies, reaching 1859 U/mL in a 50 L fermentator. It displayed the highest activity at 80 • C, and maintained more than 60% of the activity between 30 and 70 • C for 1 h. It showed an optimal pH of 10.0, and exhibited remarkable stability over a wider pH range (3.0-11.0), retaining more than 80.0% of enzyme activity for 4 h. The K m and k cat of BspPel on PGA (polygalacturonic acid) was 2.19 g L −1 and 116.1 s −1 , respectively. The activity was significantly enhanced by Ca 2+ , Mn 2+ , and Cu 2+ , and a slight increase was observed with the addition of Ba 2+ and Mg 2+. Scanning electron microscope was used to show the degumming efficiency of BspPel on ramie fibers. The loss weight was 9.2% when treated with crude enzyme supernatant and 20.8% when treated with the enzyme-chemical method, which was higher than the 14.2% weight loss in the positive control treated with 0.5% (w/v) NaOH alone. In conclusion, BspPel could be a good candidate for the ramie degumming industry.

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Section: Introductionmentioning

confidence: 98%

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Zheng

Zhang

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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“…Industrial enzyme's global market is growing day by day and in the world market, the pectinases production accounts for about 10% of the total enzyme production. By 2021 the market is estimated to reach around 35.5 million dollars [7]. In altering enzyme properties pH has a definite role.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Alkaline Pectinase Productive Bacillus tropicus from Fruit and Vegetable Waste Dump Soil

Thakur

Singh

Mukherjee

2021

Braz. arch. biol. technol.

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Alkaline pectinase is the utmost significant industrial enzyme of the bioscouring process. By considering bio scouring of cotton, 30 microbial isolates from fruit and vegetable waste rich dump soil of Solang Valley and Vasishta (Manali, Himachal Pradesh, India) were isolated and screened for the alkaline pectinase production in the current research work. Only four isolates P3, P16, P21, and P27 were capable to produce extracellular alkaline pectinase at pH 9. Further by applying submerged fermentation, the alkaline pectinase production was quantitatively screened. The most efficient isolate was P3 identified as Bacillus tropicus, based on morphological, biochemical, and molecular characterization. Molecular characteristics confirmed by 16S rDNA sequence analysis. The nucleotide sequence of the isolate was novel with a 97% similarity index and submitted to the GenBank with accession number MK332379. The Bacillus strain selected was active at broad pH range from 8-10.5 and a temperature range from 25-50 o C. HIGHLIGHTS Alkaline pectinase is very significant commercial enzyme used in bioscouring process. In traditional scouring, textile industry utilizes harmful alkaline chemicals to expel non-cellulosic material from the fabrics. Alkaline pectinase productive Bacillus tropicus was isolated from fruit and vegetable waste dump soil. Bacillus tropicus characterized by 16S rDNA sequences analysis.2 Thakur, P.; et al.

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“…Pectinase commonly referred as pectic enzyme, is a heterogenous group of enzymes such as pectolyase, pectozymes and polygalacturonase that catabolizes complex polysaccharides of plant tissues into biomolecules like galacturonic acids. [4]. Prokaryotes produce alkaline pectinases while eukaryotic microorganisms produce acidic pectinases.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Pectinase Producing Bacteria from Soil Sample

Shilpa¹,

Kaur²,

Jadon³

2021

CGCIJCTR

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The vast majority of the industrial use of enzymes is covered from microorganisms. Microorganisms are favoured in industry because of their several advantages for example rapid growth, short life expectancy and simplicity in doing genetic alterations. Microbial enzymes are thus amply provided, very much standardized and promoted by many companies. Among various enzymes, Pectinases hold an exceptional place because of its different uses in various sectors like food, textile and biofuel industries.A total of 25% of total enzyme market is being shared by Pectinase alone.The current study was carried out to evaluate the pectinase activity of the pectinolytic bacteria. 40 Bacterial strains were isolated from different soil samples and screened for Pectinase production. Primary and Secondary screening showed 3 potential isolates I38 , I39 and I40 showing pectin degradation on Vincent’s media. Further, extracellular pectinase was partially purified by ammonium sulphate precipitation and dialysis. Sequential ammonium sulphate saturations from 20-80% i.e. (20, 40, 60 and 80%) showed 60% ammonium sulphate was optimum for precipitation of intracellular enzyme whereas 80% was optimum for extracellular enzyme.

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Review on bacterial production of alkaline pectinase with special emphasis on Bacillus species

Cited by 59 publications

References 83 publications

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Isolation and Characterization of Alkaline Pectinase Productive Bacillus tropicus from Fruit and Vegetable Waste Dump Soil

Isolation and Screening of Pectinase Producing Bacteria from Soil Sample

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