Review on harnessing biotechnological tools for the development of stable bacterial wilt resistant solanaceous vegetable crops

Sharma, Shweta; Katoch, Viveka; Banyal, D. K.

doi:10.1016/j.scienta.2021.110158

Cited by 16 publications

(9 citation statements)

References 116 publications

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“…Similarly, Qian et al (2013) ; Lan et al (2014) , and Drake-Stowe et al (2017) identified four, eight, and two QTLs for TBW resistance, respectively. Thus, only 15 QTLs underlying TBW resistance have been identified, which is relatively small compared to other members of the Solanaceae family ( Sharma et al, 2021 ). Unfortunately, the identified QTLs have large genomic regions unsuitable for detecting candidate genes, and markers linked to these QTLs have limited application in tobacco breeding programs ( Li et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel Quantitative Trait Nucleotides and Candidate Genes for Bacterial Wilt Resistance in Tobacco (Nicotiana tabacum L.) Using Genotyping-by-Sequencing and Multi-Locus Genome-Wide Association Studies

Lai

Ikram

et al. 2021

Front. Plant Sci.

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Tobacco bacterial wilt (TBW) is a devastating soil-borne disease threatening the yield and quality of tobacco. However, its genetic foundations are not fully understood. In this study, we identified 126,602 high-quality single-nucleotide polymorphisms (SNPs) in 94 tobacco accessions using genotyping-by-sequencing (GBS) and a 94.56 KB linkage disequilibrium (LD) decay rate for candidate gene selection. The population structure analysis revealed two subpopulations with 37 and 57 tobacco accessions. Four multi-locus genome-wide association study (ML-GWAS) approaches identified 142 quantitative trait nucleotides (QTNs) in E1–E4 and the best linear unbiased prediction (BLUP), explaining 0.49–22.52% phenotypic variance. Of these, 38 novel stable QTNs were identified across at least two environments/methods, and their alleles showed significant TBW-DI differences. The number of superior alleles associated with TBW resistance for each accession ranged from 4 to 24; eight accessions had more than 18 superior alleles. Based on TBW-resistant alleles, the five best cross combinations were predicted, including MC133 × Ruyuan No. 1 and CO258 × ROX28. We identified 52 candidate genes around 38 QTNs related to TBW resistance based on homologous functional annotation and KEGG enrichment analysis, e.g., CYCD3;2, BSK1, Nitab4.5_0000641g0050, Nitab4.5_0000929g0030. To the best of our knowledge, this is the first comprehensive study to identify QTNs, superior alleles, and their candidate genes for breeding TBW-resistant tobacco varieties. The results provide further insight into the genetic architecture, marker-assisted selection, and functional genomics of TBW resistance, improving future breeding efforts to increase crop productivity.

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Section: Introductionmentioning

confidence: 99%

Identification of Novel Quantitative Trait Nucleotides and Candidate Genes for Bacterial Wilt Resistance in Tobacco (Nicotiana tabacum L.) Using Genotyping-by-Sequencing and Multi-Locus Genome-Wide Association Studies

Lai

Ikram

et al. 2021

Front. Plant Sci.

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“…Bacterial wilt (BW) caused by Ralstonia solanacearum infects about 250 plant species, especially plants from the Solanaceae family, which is considered a major problem in humid tropical and subtropical regions worldwide [ 1 , 2 ]. To date, various managements have been explored for the control of BW, including breeding resistant cultivars, chemical and biological controls, and soil management, but the control of this disease at a desired level through a sustainable and eco-friendly way is still awaited [ 2 – 4 ]. Breeding-resistant cultivars are still the most logical, economical, and environmental solution for suppressing BW epidemics [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…To date, various managements have been explored for the control of BW, including breeding resistant cultivars, chemical and biological controls, and soil management, but the control of this disease at a desired level through a sustainable and eco-friendly way is still awaited [ 2 – 4 ]. Breeding-resistant cultivars are still the most logical, economical, and environmental solution for suppressing BW epidemics [ 2 , 3 ]. Plant resistance to BW is very complex and controlled by QTLs.…”

Section: Introductionmentioning

confidence: 99%

“…Two other QTLs were identified on chromosomes 2 and 5, named EBWR2 and EBWR5 , respectively [ 13 – 15 ]. Although the main emphasis of BW research is on breeding resistant cultivars has been a challenge for many years [ 2 ]. Due to the breeding, cultivars resistant to BW have been restrained by polygenic inheritance, and sometimes the connection between resistance with horticultural undesirable traits related to the wild species (linkage drag) [ 7 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt

Gong,

Wang,

et al. 2023

Horticulture Research

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Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management. Here, to identify QTL controlling BW resistance, Super-BSA was performed in F2 plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193. The QTL was narrowed down through BC1F1-BC3F1 individuals by wilting symptoms and KASP markers. Rhizosphere bacterial composition of R06112, S55193, and resistant individuals EB158 (with the QTL) and susceptible individuals EB327 (without QTL) from BC2F1 generation were assessed by Illumina sequencing-based analysis, and the activation of plant immunity by the bacterial isolates was analyzed. Evidence showed that BW-resistant is controlled by one QTL located at 270 kb region on chromosome 10, namely EBWR10, and nsLTPs as candidate genes confirmed by RNA-Seq. EBWR10 has a significant effect on rhizobacteria composition and significantly recruits Bacillus. pp. A SynCom of three isolated Bacillus. pp trains significantly reduced the disease incidence, changed activities of CAT, PPO, and PAL and concentration of NO, H2O2, and O2-, activated SA and JA signaling-dependent ISR, and displayed immune activation against R. solanacearum in eggplant. Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria, which jointly promote the suppression of BW. This method charts a path to develop the QTL in resistant cultivars-driven probiotics to ameliorate plant diseases.

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“…Bacterial wilt is an exceptional threat to agriculture due to the global distribution of Rssc strains, massive crop losses associated with Rssc infections, broad host range of most Rssc strains and long persistence of Rssc strains in soil (Paudel et al ., 2020 ). Because of its massive economic impact on global agriculture, researchers have searched for and studied genetic resistance to bacterial wilt disease in many different host species (Sharma et al ., 2021 ). However, these efforts have not yet resulted in the development of a viable approach to confer broad‐spectrum resistance across all wilt‐affected crops.…”

Section: Introductionmentioning

confidence: 99%

An ancient cis‐element targeted by Ralstonia solanacearum TALE‐like effectors facilitates the development of a promoter trap that could confer broad‐spectrum wilt resistance

Gallas,

Li,

von Roepenack‐Lahaye

et al. 2023

Plant Biotechnology Journal

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SummaryRalstonia solanacearum, a species complex of bacterial plant pathogens that causes bacterial wilt, comprises four phylotypes that evolved when a founder population was split during the continental drift ~180 million years ago. Each phylotype contains strains with RipTAL proteins structurally related to transcription activator‐like (TAL) effectors from the bacterial pathogen Xanthomonas. RipTALs have evolved in geographically separated phylotypes and therefore differ in sequence and potentially functionality. Earlier work has shown that phylotype I RipTAL Brg11 targets a 17‐nucleotide effector binding element (EBE) and transcriptionally activates the downstream arginine decarboxylase (ADC) gene. The predicted DNA binding preferences of Brg11 and RipTALs from other phylotypes are similar, suggesting that most, if not all, RipTALs target the Brg11‐EBE motif and activate downstream ADC genes. Here we show that not only phylotype I RipTAL Brg11 but also RipTALs from other phylotypes activate host genes when preceded by the Brg11‐EBE motif. Furthermore, we show that Brg11 and RipTALs from other phylotypes induce the same quantitative changes of ADC‐dependent plant metabolites, suggesting that most, if not all, RipTALs induce functionally equivalent changes in host cells. Finally, we report transgenic tobacco lines in which the RipTAL‐binding motif Brg11‐EBE mediates RipTAL‐dependent transcription of the executor‐type resistance (R) gene Bs4C from pepper, thereby conferring resistance to RipTAL‐delivering R. solanacearum strains. Our results suggest that cell death‐inducing executor‐type R genes, preceded by the RipTAL‐binding motif Brg11‐EBE, could be used to genetically engineer broad‐spectrum bacterial wilt resistance in crop plants without any apparent fitness penalty.

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Review on harnessing biotechnological tools for the development of stable bacterial wilt resistant solanaceous vegetable crops

Cited by 16 publications

References 116 publications

Identification of Novel Quantitative Trait Nucleotides and Candidate Genes for Bacterial Wilt Resistance in Tobacco (Nicotiana tabacum L.) Using Genotyping-by-Sequencing and Multi-Locus Genome-Wide Association Studies

Identification of Novel Quantitative Trait Nucleotides and Candidate Genes for Bacterial Wilt Resistance in Tobacco (Nicotiana tabacum L.) Using Genotyping-by-Sequencing and Multi-Locus Genome-Wide Association Studies

A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt

An ancient cis‐element targeted by Ralstonia solanacearum TALE‐like effectors facilitates the development of a promoter trap that could confer broad‐spectrum wilt resistance

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