Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

Abstract

Abstract:In general, cell culture-based assays, investigations of cell number, viability, and metabolic activities during culture periods, are commonly performed to study the cellular responses under various culture conditions explored. Quantification of cell numbers can provide the information of cell proliferation. Cell viability study can understand the percentage of cell death under a specific tested substance. Monitoring of the metabolic activities is an important index for the study of cell physiology. Based on the development of microfluidic technology, microfluidic systems incorporated with impedance measurement technique, have been reported as a new analytical approach for cell culture-based assays. The aim of this article is to review recent developments on the impedance detection of cellular responses in micro/nano environment. These techniques provide an effective and efficient technique for cell culture-based assays.

A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

“…Beside the cell type itself (cell wall/membrane compositions, size and shape), many physical parameters, especially in the media (pH, ion concentrations), can influence the potential distribution in the double layer [20,21]. Furthermore, the given method is capable of detecting even very small numbers of bacteria in soil, food and feces-polluted water using interdigitated microelectrode designs [22,23,24,25,26,27]. …”

Low-Frequency Electrochemical Impedance Spectroscopy as a Monitoring Tool for Yeast Growth in Industrial Brewing Processes

“…Besides the cell type itself (cell wall/membrane compositions, size and shape), many physical parameters, especially in the media (pH and ion concentrations), can influence the potential distribution in the double layer [20,21]. Furthermore, the given method via α-dispersion detection is capable of detecting even very small numbers of bacteria in soil, food and feces-polluted water using interdigitated microelectrode designs [22][23][24][25][26][27]. These studies were only performed at a very small scale and with a low cell concentration.…”

Impedimetric quantification of the formation process and the chemosensitivity of cancer cell colonies suspended in 3D environment

“…Quantification of cellular responses in conventional two-dimensional (2D) culture model, that cells spread on a flat surface in a monolayer format, was demonstrated by impedance measurement across a pair of planar electrodes (Lei, 2014;Varshney and Li, 2009). If cells adhere and proliferate on the surface of the electrodes, the effective electrode surface area is reduced.…”