Review Paper: Human Tissues for Discovery Biomarker Pharmaceutical Research: The Experience of the Indiana University Simon Cancer Center—Lilly Research Labs Tissue/Fluid BioBank

Tumor banking for health research in Brazil and Latin America: time to leave the cradle

“…In a recent study, our group showed no significant change in RIN (RNA Integrity Number) values of cryopreserved tissues stored at −140°C for up to 7 years [12]. On the other hand, researchers at the Indiana University, examining sets of samples that were stored at −80°C observed that all samples with less than 18 months of storage had high-quality RNA (also analyzed by RIN), compared to only 48% of the samples that were stored for longer periods (>8 years) [8]. Currently, there is no published study that has compared the quality of macromolecules extracted from tissues that were submitted to the same collection and cryopreservation protocols and stored at −140 and −80°C, so biobanks that intend to store tissues for long periods of time should consider adopt temperatures below −130°C (either using cryogenic freezers or liquid nitrogen containers) [11,12].…”

“…It is defined as the elapsed time between the interruption of blood supply and cryopreservation of the tissue sample, and if not controlled may result in significant global gene expression changes [4][5][6]. Currently there is no universally accepted maximum cold ischemia time [7,8], and although the guidelines of the A C Camargo Biobank suggest a maximum of 30 min, collections that exceed this time are allowed in order to detect and correct deviations from the maximum time window. On average, 80% of the samples at the A C Camargo are collected within 30 min and the average collection time for the role inventory of tissue samples is 24 min.…”

“…Although many biobanks use −80°C freezers, the International Agency for Research on Cancer (IARC) recommends that this temperature should be below -130°C (which is the glass transition temperature of water) [7,8]. In theory, tissues that are quickly frozen and kept below this temperature are free of any degradation of macromolecules (e.g., hydrolysis or enzymatic degradation) [9][10][11].…”

Preclinical Models of Wound Healing: Is Man the Model? Proceedings of the Wound Healing Society Symposium

“…A RIN > 6 is considered acceptable for banked specimens. 3 He stressed the significance of robust data encryption to protect patient identities and the importance of including the ability to contact patients in the future as part of the Institutional Review Board protocol for the tissue bank.…”

Using molecular profiled human tissue to accelerate drug discovery

“…The importance of human tissues is recognized throughout the drug discovery process from the discovery phase to on-going clinical trials. In preclinical work, genetically profiled human tissue enables researchers to better understand the basis of disease heterogeneity, select and validate new drug targets, find genetic variations contributing to disease and validate animal disease models [1]. Additionally, the integration of this biological data (genomics, transcriptomics, proteomics, etc.)…”