Review: Seaweed tissue culture as applied to biotechnology: Problems, achievements and prospects

Abstract

Advances have been made in cell and tissue culture of seaweeds to define a unique branch of in vitro techniques; however, they are lagging far behind those of land plants and have limited applications. Explants can be cultivated axenically in enriched or artificial seawater culture media, and regeneration and even callus formation are achieved. In this state of the art technique, seaweed tissue culture may be already useful for certain biotechnological applications, such as clonal propagation of seed material for mariculture. Nevertheless, the absolute control of growth and development as it is exerted in higher plant tissue culture is lacking, and it is required for more complex biotechnological applications in seaweeds. Definitively, we need appropriate cells (competent cells) to induce growth with the most effective chemical regulators in culture medium adjusted towards the addition of carbon sources. Still, free cells and protoplast isolation and regeneration in marine seaweeds constitute the most developed topic in seaweed tissue culture. The regulation of growth and development of seaweed free cell and protoplast cultures may sustain a purposeful use of techniques in the era of genomic applications.

Regeneration Studies in Grateloupia filicina (J.V. Lamouroux) C. Agardh - An Important Carrageenophyte and Edible Seaweed

“…Regeneration is a wide spread phenomenon in seaweeds which demonstrates totipotency of algal cells, provides a mean for studying morphogenetic stages of adventive embryony, and produces correlation growth effects which may be useful for hypothesizing mechanism of regulation of morphogenesis (Buggeln 1981;Baweja et al 2009). The development of in vitro vegetative propagation permits the rapid multiplication of desired varieties.…”

EVALUASI PERFORMA BIBIT RUMPUT LAUT Gracilaria verrucosa HASIL KULTUR JARINGAN DI KABUPATEN LUWU, SULAWESI SELATAN

“…Kriteria bibit yang baik untuk kultur jaringan adalah rumput laut yang memiliki thalus segar, tidak berlendir dan bebas dari epyfit. Penggunaan bibit dari hasil seleksi klon untuk penyediaan bibit melalui kultur jaringan diharapkan dapat menghasilkan bibit yang memiliki kualitas baik sesuai dengan pendapat Baweja et al (2009) yang menyatakan bahwa kombinasi metode kultur jaringan dengan metode penyediaan bibit yang lain dapat meningkatkan kualitas bibit rumput laut.…”

Polyamines increase carpospore output and growth during in vitro cultivation of Hydropuntia cornea

“…To overcome this problem, control over reproduction to introduce new cultivation techniques using gamete or spore seeding is a necessity in most large scale algae cultivation systems ). An increasing number of studies have dealt with algae tissue culture following the techniques used in higher plants (Gao et al 2005;Baweja et al 2009). Although there are many reports on callus induction, thallus regeneration and growth from a wide variety of economically important algae, far less is known about hormonal regulation of development, morphogenesis and regeneration.…”