Review: the potential impact of surface crystalline states of titanium for biomedical applications

Barthès, Julien; Ciftci, Sait; Ponzio, Florian; Knopf‐Marques, Helena; Pelyhe, Liza; Gudima, Alexandru; Kientzl, Imre; Bognár, Eszter; Weszl, Miklós; Kzhyshkowska, Julia; Vrana, Nihal Engin

doi:10.1080/07388551.2017.1363707

Cited by 22 publications

(15 citation statements)

References 129 publications

(110 reference statements)

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“…It is well known that biomaterials surface properties (such as chemistry, morphology, roughness, wettability) play an essential role in the association between implants and biomaterials. It is found that both morphology diversity and crystalline phases could affect cell responses to TNTs [ 13 , 23 , 24 ]. However, there is no consistency in the literature.…”

Section: Introductionmentioning

confidence: 99%

Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes

Wang

Dong

et al. 2020

Bioactive Materials

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The research of TiO 2 nanotubes (TNTs) in the field of biomedicine has been increasingly active. However, given the diversity of the nanoscale dimension and controversial reports, our understanding of the structure-property relationships of TNTs is not yet complete. In this paper, gradient TNTs with a wide diameter range of 20–350 nm were achieved by bipolar electrochemistry and utilized for a thorough high-throughput study of the effect of nanotube dimension and crystalline phase on protein adsorption and cell behaviors. Results indicated that protein adsorption escalated with nanotube dimension whereas cell proliferation and differentiation are preferred on small diameter (<70 nm) nanotubes. Large diameter anatase nanotubes had higher adsorption of serum proteins than as-prepared ones. But only as-prepared small diameter nanotubes presented slightly higher cell proliferation than corresponding annealed nanotubes whereas there was no discernible difference between as-prepared and annealed nanotubes on cell differentiation for the entire gradient. Those findings replenish previous research about how cell responses to TNTs with a wide diameter range and provide scientific guidance for the optimal design of biomedical materials.

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Section: Introductionmentioning

confidence: 99%

Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes

Wang

Dong

et al. 2020

Bioactive Materials

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“…Subsequently, an increasing amount of effort has been put into exploring structure-property relationships between cell responses and TNT properties, mainly in investigating the size effect of TNTs on cell responses (Lv et al 2015;Oh et al 2009;Park et al 2009;Pittrof et al 2012;Yu et al 2018;Zhao et al 2012). Crystalline phase, on the other hand, also has been proved to play a key role in electrochemical properties (Kulkarni et al 2015b) cell behaviors, inflammatory (Barthes et al 2018), and blood compatibility (Maitz et al 2003;Zhang et al 2018). The TiO 2 group consists of anatase (tetragonal), rutile (tetragonal), and brookite (rhombic).…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of crystalline phase on protein adsorption and cell behaviors on TiO2 nanotubes

Dong

Zhang

et al. 2019

Appl Nanosci

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The objective of this study is to explore the structure-property relationships of TiO 2 nanotubes (TNTs) with different crystalline phases that link to protein adsorption and cell responses. Given the formation of intact rutile nanotubular structures by furnace annealing is challenge, a combination of furnace annealing and flame annealing is employed for the preparation of rutile TNTs. TNTs with pure anatase phase and mixed anatase/rutile phases are obtained by simple furnace annealing of amorphous TNTs. Results show that BSA and FBS adsorptions are greatly enhanced on rutile TNTs, whereas no discernable difference on other crystalline phases. Rutile TNTs also present highest adsorption of fibronectin and collagen which are diminished on anatase and dual anatase-rutile phases. Interestingly, however, there is no significant difference in cell proliferation or differentiation on TNTs with different crystallites. Scrutinization of the surface properties involved in protein adsorption and cell activities, a synergistic effect of surface charge, hydroxyl groups, and roughness is found on protein adsorption which further regulates cell behaviors. Those findings provide a better understanding of the structure-property relationships of titanium-based biomaterials.

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“…Background Titanium (Ti) and its alloys are the biomaterials mostly used in joint replacements due to their excellent biocompatibility [1][2]. However, one of the main drawbacks to Ti is its susceptibility to both mechanical wear (due to cyclic loading) and corrosion (due to the contact with biological uids) events resulting in the release of wear debris and corrosion products that give rise to local and systemic complications to patients [3][4][5].…”

Section: Discussionmentioning

confidence: 99%

Osteoblasts-derived Exosomes as Novel Communicators in Particle Induced Periprosthetic Osteolysis

Souza

Gemini-Piperni

Ruivo

et al. 2021

Preprint

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BackgroundThe inflammatory response to titanium implant-derived wear particles is considered as the hallmark of periprosthetic osteolysis, an event that cause pain, reduce patient motility, ultimately leading to the need of a revision surgery. Although macrophages are major cell players, other cell types such as bone cells can indirectly contribute to periprosthetic osteolysis, however the mechanisms are not fully understood. Exosomes (Exos) has been related with several bone pathologies, with growing body of literature recognizing them as actively shuttle molecules through the body, with their cargo being completely dependent of external stimuli (e.g. chemicals and metals ions and particles). Till the moment, the role of wear debris on osteoblasts exosomes biogenesis is absent and the possible contribution of Exos to osteoimmune communication and periprosthetic osteolysis is still in its infancy. Taking that in consideration, in this work we investigate the effect of wear debris on Exo biogenesis, where two bone cell models were exposed to titanium dioxide nanoparticles (TiO2 NPs) similar in size and composition to wear debris associated with prosthetic implants. The contribution of Exos to periprosthetic osteolysis was evaluated performing functional tests stimulating primary human macrophages with bone-derived Exos.ResultsFor the first time, we report that TiO2 NPs enter in multivesicular bodies, the nascent of Exos and altered osteoblasts derived exosomes secretion and cargo. No significant differences were observed in Exos morphology and size, however mass spectrometry analysis identified urokinase-type plasminogen activator (uPA), specifically enriched in Exos derived from bone cells pre-incubated with TiO2 NPs. Functional tests confirmed the activation of human macrophages towards a mixed phenotype with consequent secretion of pro and anti-inflammatory cytokines. ConclusionsThe external stimuli of osteoblasts to TiO2 NPs induced a dose dependent secretion of Exos, suggesting alterations in their biogenesis as well as in their cargo. Functional tests reveal that enriched uPA exosomal cargo is stimulating macrophages towards a mixed M1 and M2 phenotype inducing the release of pro-and anti-inflammatory signals that are characteristic of periprosthetic osteolysis. Interestingly, uPA may be proposed, in the future, as a possible candidate biomarker to early diagnose particle induced periprosthetic osteolysis, since uPA was also detected in the pseudocapsular interface around implants of patients with loosening of total hip prosthesis and joint replacement surgery, suggesting their active role in disease progression.

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Review: the potential impact of surface crystalline states of titanium for biomedical applications

Cited by 22 publications

References 129 publications

Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes

Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes

Synergistic effect of crystalline phase on protein adsorption and cell behaviors on TiO2 nanotubes

Osteoblasts-derived Exosomes as Novel Communicators in Particle Induced Periprosthetic Osteolysis

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