Review: The Role of MicroRNAs in Osteoarthritis and Chondrogenesis

Le, Linh; Swingler, T.E.; Clark, Ian M.

doi:10.1002/art.37990

Cited by 108 publications

(93 citation statements)

References 105 publications

(182 reference statements)

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“…6 7 Aberrant miRNA expression or dysregulation of miRNA processing has been linked to many pathologies, including cancer, cardiovascular or rheumatic disease. [7][8][9] Since miRNA are protected from RNAse activity in the peripheral blood, miRNAs may serve as excellent biomarkers for physiological and pathological processes. 10 11 Although dysregulated miRNA expression has been well documented in the osteoarthritic cartilage, 9 12-17 evidence on altered circulating miRNA levels in OA patients is very limited.…”

Section: Introductionmentioning

confidence: 99%

“…[7][8][9] Since miRNA are protected from RNAse activity in the peripheral blood, miRNAs may serve as excellent biomarkers for physiological and pathological processes. 10 11 Although dysregulated miRNA expression has been well documented in the osteoarthritic cartilage, 9 12-17 evidence on altered circulating miRNA levels in OA patients is very limited. 18 19 Hence, our project is the first to investigate the differential expression of circulating miRNAs in subjects with and without incident OA in a large prospective population-based cohort.…”

Section: Introductionmentioning

confidence: 99%

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Signature of circulating microRNAs in osteoarthritis

et al. 2014

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Background Osteoarthritis is the most common form of arthritis and a major socioeconomic burden. Our study is the first to explore the association between serum microRNA levels and the development of severe osteoarthritis of the knee and hip joint in the general population. Methods We followed 816 Caucasian individuals from 1995 to 2010 and assessed joint arthroplasty as a definitive outcome of severe osteoarthritis of the knee and hip. After a microarray screen, we validated 12 microRNAs by real-time PCR in the entire cohort at baseline. Results In Cox regression analysis, three microRNAs were associated with severe knee and hip osteoarthritis. let-7e was a negative predictor for total joint arthroplasty with an adjusted HR of 0.75 (95% CI 0.58 to 0.96; p=0.021) when normalised to U6, and 0.76 (95% CI 0.6 to 0.97; p=0.026) after normalisation to the Ct average. miRNA-454 was inversely correlated with severe knee or hip osteoarthritis with an adjusted HR of 0.77 (95% CI 0.61 to 0.97; p=0.028) when normalised to U6. This correlation was lost when data were normalised to Ct average (p=0.118). Finally, miRNA-885-5p showed a trend towards a positive relationship with arthroplasty when normalised to U6 (HR 1.24; 95% CI 0.95 to 1.62; p=0.107) or to Ct average (HR 1.30; 95% CI 0.99 to 1.70; p=0.056). Conclusions Our study is the first to identify differentially expressed circulating microRNAs in osteoarthritis patients necessitating arthroplasty in a large, population-based cohort. Among these microRNAs, let-7e emerged as potential predictor for severe knee or hip osteoarthritis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Signature of circulating microRNAs in osteoarthritis

et al. 2014

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“…The level of expressed miRNAs of interest, rno-miR-199a-3p, rno-miR-181a-5p, rno-miR-140-3p and rno-miR-27b- Figure 5A and 5B). It have been reported that miR-140 is specifically expressed in cartilage tissue to activate or supress PDGF, SOX9, Sp1, aggrecan, VEGF and HDAC4 [28,41]. Several miRNAs including miR-140, miR-199a, mir-193 and mir-29a/29b control the anabolic and catabolic regulation in cartilage [42].…”

Section: Principal Component Analysis and Relationship Of Mirnasmentioning

confidence: 99%

Next-generation Sequencing Identifies Articular Cartilage and Subchondral Bone Mirnas after ESWT on Early Osteoarthritis Knee

Wang

Cheng

et al. 2017

Osteoarthritis and Cartilage

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Extracorporeal shockwave therapy (ESWT) has shown chondroprotective effects on the initiation of the osteoarthritis (OA) changes of the rat knee. This study evaluated 69 significant expressed profiles of microRNA (miRNA) in the articular cartilage and subchondral bone after ESWT. There were 118 target genes identified for miRNAs of interest in articular cartilage and 214 target genes in subchondral bone by next generation sequencing (NGS). In principal component analysis (PCA), the relationships of miRNA expression in bone and cartilage were improved after ESWT. Global functional annotation showed that predicted targets were involved in cartilage development, inflammatory and immune response, ion binding, angiogenesis, cell adhesion, cell cycle, transcription and translation, gene expression, NTP binding, signal transduction, collagen fibril organization, apoptotic process, chondrocyte differentiation, cell differentiation, bone development as well as cell proliferation. The miRNAs profile and the target genes were comprehensively surveyed and compared in articular cartilage and subchondral bone of early OA knee before and after ESWT. Our study represents the direct assessment to date of miRNA expression profiling in early OA articular cartilage and subchondral bone. The results provide insights that could contribute to the development of new biomarkers and therapeutic strategies for OA changes and the treatment with ESWT.

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“…These studies started with an investigation of the epigenetic regulation of the expression of genes coding for proteins that have a known central role in the homeostasis of joint tissue, and in particular cartilage (14). There have also been direct investigations of the differences in expression levels of regulatory RNAs between OA and non-OA cells, the aim being to identify key RNAs that could initiate or exacerbate disease development (15,16). The advent of high-throughput CpG DNA methylation arrays has enabled investigators to directly compare, at a genome-wide level, OA and non-OA methylomes and to compare methylation status between DNA from different sites within the joint and between different joints (17)(18)(19)(20)(21)(22).…”

Section: Epigenetics and Oamentioning

confidence: 99%