Review: The Self-Assembly Approach for Organ Reconstruction by Tissue Engineering

Auger, François A.; Rémy-Zolghadri, Murielle; Grenier, Geneviève; Germain, Lucie

doi:10.1089/152489000414642

Cited by 36 publications

(15 citation statements)

References 77 publications

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“…This method is based on the capacity of fibroblasts to produce their own extracellular matrix. Thus, it allows for the production of skin substitutes exempt of exogenous material [39,40]. Their epidermal layer is fully differentiated with a well-defined stratum corneum, which shows a good barrier function [41].…”

Section: Discussionmentioning

confidence: 99%

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Pouliot¹,

Angers²,

Dubois-Declercq³

et al. 2017

JDC

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Short AbstractThree-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermopharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitutes produced by tissue engineering. IntroductionLocated at the interface between the human body and the exterior environment, skin is the first line of defense against pathogens, chemicals, and harmful mechanical stimuli [1]. Due to its large surface area and its structural and functional complexity, regenerating a complete human skin represents a big challenge. Over the past years, different human skin substitute models have evolved in order to treat victims of severe burns and chronic cutaneous wounds [2]. Skin substitutes are useful in both applied and fundamental fields of research. They can be grafted as skin replacement in clinical applications or used for various types of skin tests, such as dermopharmacology and toxicology studies [2,3]. Skin substitutes properties must be as close as possible to those of normal skin [4].Over the past decade or so, the use of animals in fundamental and applied research has been subject to social debates, and this has led to the creation of more restrictive legislations, such as the addition of the Seventh Amendment to the European Union's Cosmetic Directive [4]. Utilization of skin substitutes to perform tests allowed for the elimination, or at least reduction, of controversial animal testing. Moreover, as animal skin differs in many ways to human skin, well representative skin substitutes could improve the validity of performed tests [5]. In order to increase efficiency, rapid access to skin substitutes is critical. Therefore, optimized storage methods are needed [6].Cryopreservation is an approach that has been under much investigation recently. The impact of various freezing conditions has been tested on animal and human skins. Among all tests performed, permeability analysis is one of the most commonly used in the literature. This test is based on the fact that skin, despite its crucial involvement as a barrier against exogenous material, remains permeable to most substances. AbstractBackground: Three-dimensional skin substitutes reconstructed by tissue engineering have strong potential to emulate skin conditions in vivo, although their production necessitates a relatively long period of time. Storage of cryopreserved substitutes among time, using some kind of banking system, would be a conceivable solution to make their utilization more appealing for dermo pharmaceutical testing. This study evaluated the effects of freezing at -20 °C over a period of 2 months on the structural and physicochemical properties of skin substitu...

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Section: Discussionmentioning

confidence: 99%

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Pouliot¹,

Angers²,

Dubois-Declercq³

et al. 2017

JDC

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“…For example, using the elastomeric micropatterned stamps, molecules can be deposited in precise patterns via microcontact printing in order to spatially control cell adhesion (Williams et al, 2009;Kane et al, 1999;Chen et al, 1998), or the stamps themselves can be used as substrata that provide topological cues (Sarkar et al, 2005;Glawe et al, 2005;Vernon et al, 2005;Zorlutuna et al, 2009;Fujita et al, 2009); however, bridging the gap between these cell-scale technologies and macroscale tissue engineering has yet to be realized. Cell sheet engineering has arisen recently as an attractive approach to tissue engineering in which confluent cell cultures are harvested from a variety of substrates as intact, tissue-like sheets that have the advantage of consisting solely of cells and their associated, cell-secreted ECM (i.e., no synthetic materials) (Yang et al, 2007;Auger et al, 2000). Although this technology has proven to be a highly effective means of engineering tissues, the resulting cell sheets lack structure due to a lack of organizational cues from the substrates.…”

Section: Introductionmentioning

confidence: 99%

Micropatterned cell sheets with defined cell and extracellular matrix orientation exhibit anisotropic mechanical properties

Isenberg

Backman

Kinahan

et al. 2012

Journal of Biomechanics

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“…In this approach, confluent cell cultures are harvested from a variety of substrates as intact, tissuelike sheets consisting of the cells and their associated extracellular matrix (ECM) [16,17]. These sheets can then be used individually or layered/rolled to create tissues of larger size or with defined laminar organization.…”

Section: Introductionmentioning

confidence: 99%

A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization

et al. 2008

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The proper function of many tissues depends critically on the structural organization of the cells and matrix of which they are comprised. Therefore, in order to engineer functional tissue equivalents that closely mimic the unique properties of native tissues it is necessary to develop strategies for reproducing the complex, highly organized structure of these tissues. To this end, we sought to develop a simple method for generating cell sheets that have defined ECM/cell organization using microtextured, thermoresponsive polystyrene substrates to guide cell organization and tissue growth. The patterns consisted of large arrays of alternating grooves and ridges (50 μm wide, 5 μm deep). Vascular smooth muscle cells cultured on these substrates produced intact sheets consisting of cells that exhibited strong alignment in the direction of the micropattern. These sheets could be readily transferred from patterned substrates to non-patterned substrates without the loss of tissue organization. Ultimately, such sheets will be layered to form larger tissues with defined ECM/cell organization that spans multiple length scales.

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Review: The Self-Assembly Approach for Organ Reconstruction by Tissue Engineering

Cited by 36 publications

References 77 publications

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Effects of Freezing on Functionality and Physicochemical Properties of A 3D-Human Skin Model

Micropatterned cell sheets with defined cell and extracellular matrix orientation exhibit anisotropic mechanical properties

A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization

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