Reviews of Science for Science Librarians: The Conservation of Endangered Plants Using Micropropagation

Jaskowiak, Megan

doi:10.1080/0194262x.2013.872590

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…In vitro techniques can be used to multiply rare or endangered plants asexually [ 12 , 14 , 15 , 16 ]. Micropropagation has many advantages, including rapid production of identical plants.…”

Section: Introductionmentioning

confidence: 99%

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Issac

Kuriakose

Leung

et al. 2021

Plants

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Xerophyllum asphodeloides (Xerophyllaceae), known as eastern turkeybeard, is an herbaceous perennial found in eastern North America. Due to decline and destruction of its habitat, several states rank X. asphodeloides as “Imperiled” to “Critically Imperiled”. Protocols for seed cryopreservation, in vitro germination, sustainable shoot micropropagation, shoot establishment in soil, and seed germination are presented. Seeds from two tested sources were viable after 20 months of cryopreservation. Germination of isolated embryos in vitro was necessary to overcome strong seed dormancy. Shoot multiplication and elongation occurred on ½ MS medium without PGRs. Shoots rooted in vitro without PGRs or with 0.5 mg/L NAA or after NAA rooting powder treatment and placement in potting mix. When planted in wet, peaty soil mixes, shoots grew for two months and then declined. When planted in a drier planting mix containing aged bark, most plants continued growth. In the field, plant survival was 73% after three growing seasons. Safeguarding this species both ex situ and in situ is possible and offers a successful approach to conservation. Whole seeds germinated after double dormancy was overcome by incubation under warm moist conditions for 12 weeks followed by 12 weeks cold at 4 °C and then warm.

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Section: Introductionmentioning

confidence: 99%

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Issac

Kuriakose

Leung

et al. 2021

Plants

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In vitro propagation and conservation of wild bilberry (Vaccinium myrtillus L.) genotypes collected in the Tuscan Apennines (Italy)

Nin

Benelli̇

Petrucci

et al. 2019

JBR

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BACKGROUND: Vaccinium myrtillus L. is a valuable wild fruit species of conservation concern in the Tuscan Apennines (Italy). OBJECTIVE:The development of a micropropagation protocol for the conservation of this species in in vitro-banks for the year-round mass production of specific selected superior bilberry genotypes, maintaining the genetic variability of natural populations, avoiding genetic erosion. METHODS: Nodal segments from seedlings were cultured on mMS medium with zeatin or 2iP for multiplication and elongation stage, afterwards shoots were rooting in vitro and in vivo. The RAPD analysis was applied to lines obtained and slow growth storage (4 • C -darkness) was evaluated for the conservation. RESULTS: Zeatin concentration 9.1 M was the most favorable for shoot proliferation. The activated charcoal was essential for in vitro rooting. Both in vitro and ex vitro methods were successfully used to root and acclimatize bilberry shoots. The RAPD analysis indicated a large genetic diversity among the micropropagated genotypes. Shoots were successfully in vitro preserved for up to 6 months with no apparent deterioration effect. CONCLUSIONS: In vitro propagation and conservation are fundamental tools for exploiting the whole potential of this wild crop species for breeding and genetic improvement purposes.

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Threatened Woody Plants of Georgia and Micropropagation as a Tool for In Vitro Conservation

Gaidamashvili

Benelli̇

2021

Agronomy

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Georgia is the major part of the Caucasus; it is considered as one of the distinguished regions of the world with respect to biodiversity. The majority of Georgia’s biodiversity is connected with forest ecosystems, which cover about 38% of the country’s territories. In Georgia, as in other countries, many unique species of forest phytocenosis are threatened by extinction and/or genetic pollution due to the negative impact of various environmental and anthropogenic factors. Implementation of biotechnological approaches in practice for in vitro conservation of species can significantly speed up the processes of protection, thus guaranteeing the sustainability of the phytogenetic pool of the country. The present review summarizes the current status of several threatened woody perennials of the Red List of Georgia belonging to the genera Castanea, Quercus, and Betula, which are the dominant or edificatory species of forest phytocenosis. The feasibility for their in vitro propagation for conservation purposes is discussed.

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Reviews of Science for Science Librarians: The Conservation of Endangered Plants Using Micropropagation

Cited by 3 publications

References 12 publications

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

Seed Cryopreservation, Germination, and Micropropagation of Eastern Turkeybeard, Xerophyllum asphodeloides (L.) Nutt.: A Threatened Species from the Southeastern United States

In vitro propagation and conservation of wild bilberry (Vaccinium myrtillus L.) genotypes collected in the Tuscan Apennines (Italy)

Threatened Woody Plants of Georgia and Micropropagation as a Tool for In Vitro Conservation

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