Revised nomenclature and functional overview of the ULP gene family of plant deSUMOylating proteases

Castro, Pedro Humberto; Bachmair, Andreas; Bejarano, Eduardo R.; Coupland, George; Lois, L. María; Sadanandom, Ari; Burg, H.A. van den; Vierstra, Richard D.; Azevedo, Herlânder

doi:10.1093/jxb/ery301

Cited by 23 publications

(23 citation statements)

References 36 publications

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“…The SUMO protease genes in peanut could be classified into four groups: Class I ELS-type, Class II OTS-type, Class III FUG-type, and Class IV SPF-type ( Fig. 4), which were consistent with the classification results in Arabidopsis [46,47]. Furthermore, all of them were found to have a peptidase_C48 domain (Additional file 8: Figure S8), inferring similar SUMO protease activity.…”

Section: Identification and Characterization Of Peanut Sumo Pathway Gsupporting

confidence: 76%

“…However, MMS21 genes were expressed poorly in nearly all tissues, indicating that the role of MMS21 in peanut is mainly possibly focused on DNA repair. SUMO proteases, which have deSUMOylation activity, also play important regulatory roles in plant development and stress responses [51,56]. For example, ESD4 was found to participate in the regulation of flowering of Arabidopsis [57].…”

Section: Discussionmentioning

confidence: 99%

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Defining the function of SUMO system in pod development and abiotic stresses in Peanut

et al. 2019

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Background: Posttranslational modification of proteins by small ubiquitin like modifier (SUMO) proteins play an important role during the developmental process and in response to abiotic stresses in plants. However, little is known about SUMOylation in peanut (Arachis hypogaea L.), one of the world's major food legume crops. In this study, we characterized the SUMOylation system from the diploid progenitor genomes of peanut, Arachis duranensis (AA) and Arachis ipaensis (BB). Results: Genome-wide analysis revealed the presence of 40 SUMO system genes in A. duranensis and A. ipaensis. Our results showed that peanut also encodes a novel class II isotype of the SCE1, which was previously reported to be uniquely present in cereals. RNA-seq data showed that the core components of the SUMOylation cascade SUMO1/2 and SCE1 genes exhibited pod-specific expression patterns, implying coordinated regulation during pod development. Furthermore, both transcripts and conjugate profiles revealed that SUMOylation has significant roles during the pod development. Moreover, dynamic changes in the SUMO conjugates were observed in response to abiotic stresses. Conclusions: The identification and organization of peanut SUMO system revealed SUMOylation has important roles during stress defense and pod development. The present study will serve as a resource for providing new strategies to enhance agronomic yield and reveal the mechanism of peanut pod development.

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Section: Identification and Characterization Of Peanut Sumo Pathway Gsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Defining the function of SUMO system in pod development and abiotic stresses in Peanut

et al. 2019

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“…Following the evidence that SUMO proteases are important for DELLA activity (Campanaro et al, 2016), the knockout mutant of SUMO protease fug1 showed a higher ratio of overall SUMOylated SLR1. Interestingly, the FUG subclass is phylogenetically close to the SPF subclass, which contains Ulp2-like SUMO proteases (Castro et al, 2018). A Ulp2 from Saccharomyces cerevisiae (ScUlp2) was previously reported to be the key protease responsible for poly-SUMO chain processing (Eckhoff and Dohmen, 2015).…”

Section: Discussionmentioning

confidence: 99%

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

Gonçalves¹,

Fernandes²,

Nunes

et al. 2020

Preprint

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Author Contributions: 15 NMG participated in the conception of research, executed most experiments, and 16 wrote the article with contributions from all authors. TF did protein interaction 17 experiments. MTGR characterized SUMO-protease rice lines. CCM analysed data 18 and completed the writing. MAAR purified recombinant proteins. PMB analysed RNA 19 seq. MMO supervised experiments and analysed results. IAA conceived original 20 project, supervised experiments, and finalised the article. 31 32 Short title (50 characters): SUMOylation of rice SLR1 increases salt tolerance 33 34 ABSTRACT 35 DELLA proteins modulate GA signalling and are major regulators of plant plasticity to 36 endure stress. DELLAs are mostly regulated at the post-translational level, and their 37 activity relies on the interaction with upstream regulators and transcription factors 38 (TFs). SUMOylation is a post-translational modification (PTM) capable of changing 39protein interaction and found to influence DELLA activity in Arabidopsis. We 40 determined that SUMOylation of the single rice DELLA SLENDER RICE1 (SLR1) 41 occurs in a lysine residue different from the one identified in Arabidopsis 42 REPRESSOR OF GA (RGA), which suggests differential DELLA regulation by 43SUMOylation in monocots and dicots. Remarkably, artificially increasing 44 SUMOylated SLR1 (SUMO1SLR1) levels attenuated the penalty of salt stress on 45 plant yield. Gene expression analysis revealed that the overexpression of 46 SUMOylated SLR1 regulates key dioxygenases that modulate active GA levels, 47namely GA20ox2 and GA2ox3, which could partially explain the sustained 48 productivity upon salt stress imposition. Besides, SLR1 SUMOylation blocked the 49 interaction with the growth regulator YAB4, which may fine-tune GA20ox2 50 expression. Mechanistically, we propose that SLR1 SUMOylation disrupts the 51 interaction with members of several transcription factor families to modulate gene 52 expression. We found that SLR1 SUMOylation represents a novel mechanism 53 modulating DELLA activity, which attenuates the impact of stress on plant 54 performance. 55 56 gibberellin 58 59 One sentence summary: Rice plants show increased yield under salt stress when 60 its gibberellin transcriptional regulator DELLA protein is artificially SUMOylated.

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“…Given the importance of SUMO proteases, a further Viewpoint in the eXtra Botany section, by Castro et al (2018 b ), reports on a community-wide effort to revise the nomenclature of a subgroup of these enzymes based on a new evolutionary phylogenetic assessment. Such community efforts facilitate forthcoming research in this area and ensure that newly identified enzymes from a variety of crop species will be studied appropriately.…”

Section: Exploiting Ptms In Crop Biotechnologymentioning

confidence: 99%

Orchestrating the proteome with post-translational modifications

Spoel

2018

Journal of Experimental Botany

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Revised nomenclature and functional overview of the ULP gene family of plant deSUMOylating proteases

Cited by 23 publications

References 36 publications

Defining the function of SUMO system in pod development and abiotic stresses in Peanut

Defining the function of SUMO system in pod development and abiotic stresses in Peanut

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

Orchestrating the proteome with post-translational modifications

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