Revisions in the technique of epi-illumination light microscopy for the study of floral and vegetative apices

Posluszny, Usher; Scott, Martha G.; Sattler, Rolf

doi:10.1139/b80-290

Cited by 79 publications

(42 citation statements)

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“…76.0083) and from the Botanical Garden, Erlangen, Germany. Development was studied with the epi-illumination, light microscopy technique of Sattler (1968) and Posluszny et al (1980) and with SEM. Material for epi-illumination study was fixed in FAA (Berlyn and Miksche 1976), dehydrated to 100% ethanol, and stained for several days to weeks in Johansen's Fast Green (Johansen 1940).…”

Section: Methodsmentioning

confidence: 99%

Inflorescence and Floral Development in Orchidantha maxillarioides (Lowiaceae)

Kirchoff¹,

Kunze²

1995

International Journal of Plant Sciences

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Section: Methodsmentioning

confidence: 99%

Inflorescence and Floral Development in Orchidantha maxillarioides (Lowiaceae)

Kirchoff¹,

Kunze²

1995

International Journal of Plant Sciences

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“…Vegetative and inflorescence apices were fixed in Formalin -acetic acid -alcohol (FAA: 50 mL 95 % ethanol, 5 mL glacial acetic acid, 10 mL 40% Formalin, 35 mL H20). An epi-illumination, light microscopy "technique (Sattler 1968;Posluszny et al 1980) was used in this study. Fixed material was dehydrated to 100% ethanol and stained for 1 week in Johansen's fast green (Johansen 1940).…”

Section: Methodsmentioning

confidence: 99%

Inflorescence structure and development in the Zingiberales: Thalia geniculata (Marantaceae)

Kirchoff¹

1986

Can. J. Bot.

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The inflorescence of Thalia geniculata is among the most complex in a family characterized by complex inflorescences. The main axis of the inflorescence bears primary bracts which subtend either enriching branches, in the enriching zone, or pairs of flowers, in the florescence. The enriching branches repeat the structure of the inflorescence as a whole, with the addition of two specialized bracts: a prophyll and an interphyll. The paired flowers of the Marantaceae are mirror images of each other. The first bract of the inflorescence is formed in the plane of distichy of the leaves. Subsequent bracts are formed slightly to one side of the apex, giving a one-sided distichous arrangement. On an enriching branch the sterile prophyll is formed adaxially, followed by a fertile, abaxial interphyll. The first bract following the interphyll lies in a plane approximately perpendicular to the plane of the prophyll and interphyll. With the second bract the alternating divergence angles of approximately 115 and 145° begin to be established. The paired flowers of the florescence are subtended by a single, adaxial prophyll. Unlike many other Marantaceae no interphyll or bracteoles are formed below the flowers.

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“…The fixed inflorescences of both mutant and wildtype plants were transferred for the epi-illumination analysis through several changes of a solution of 70% ethanol before being stained for 24 -72 h in 0.5% (w/v) alcoholic acid fuchsin (in 95% ethanol). The excess of dye was removed from the tissue by destaining in an ethanol series (Posluszny et al, 1980). Dissection of the tissue was carried out in 100% ethanol to reveal the receptacle.…”

Section: Histological Analysis and Epi-illumination Analysismentioning

confidence: 99%

“…Dissection of the tissue was carried out in 100% ethanol to reveal the receptacle. Mounting of plant material and photographs were performed as described by Posluszny et al (1980).…”

Section: Histological Analysis and Epi-illumination Analysismentioning

confidence: 99%

MISSING FLOWERS gene controls axillary meristems initiation in sunflower

Fambrini

Cionini

Bertini

et al. 2003

Genesis

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The initiation and growth of axillary meristems are fundamental components of plant architecture. Here, we describe the mutant missing flowers (mf) of Helianthus annuus characterized by the lack of axillary shoots. Decapitation experiments and histological analysis indicate that this phenotype is the result of a defect in axillary meristem initiation. In addition to shoot branching, mutation affects floral differentiation. The indeterminate inflorescence of sunflower (capitulum) is formed of a large flat meristem which produces floret primordia in multiple spirals. In wildtype plants a bisecting crease divides each primordium in two distinct bumps that adopt different fate. The peripheral (abaxial) part of the primordium becomes a small leaf-like bract and the adaxial part becomes a flower. In the mf mutant, the formation of flowers at the axil of bracts is precluded. Histological analyses show that in floret primordia of the mutant a clear subdivision in dyads is not established. The primordia progressively bend inside and only large involucral floral bracts are developed. The results suggest that the MISSING FLOWERS gene is essential to provide or perceive an appropriate signal to the initiation of axillary meristems during both vegetative and reproductive phases.

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Revisions in the technique of epi-illumination light microscopy for the study of floral and vegetative apices

Cited by 79 publications

References 0 publications

Inflorescence and Floral Development in Orchidantha maxillarioides (Lowiaceae)

Inflorescence and Floral Development in Orchidantha maxillarioides (Lowiaceae)

Inflorescence structure and development in the Zingiberales: Thalia geniculata (Marantaceae)

MISSING FLOWERS gene controls axillary meristems initiation in sunflower

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