Revisit dietary fiber on colorectal cancer: butyrate and its role on prevention and treatment

Encarnação, João Crispim; Abrantes, Ana Margarida; Pires, Ana Salomé; Botelho, Maria Filomena

doi:10.1007/s10555-015-9578-9

Cited by 101 publications

(62 citation statements)

References 93 publications

(152 reference statements)

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“…SCFAs, and particularly butyrate, are well known for their ability to inhibit proliferation and induce apoptosis of colorectal cancer cells [2,3], but in this study high levels of butyrate in FS did not always affect cell viability. The butyrate content in pectin-inulin FS was nearly two-fold higher than pectin alone FS, while the propionate and acetate content from pectin-inulin FS was nearly double of pectin FS, however, the effect on Caco-2 cell growth when cultured in media containing these fermentation supernatants was no different.…”

Section: Discussioncontrasting

confidence: 55%

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Analysis of the Anti-Cancer Effects of Cincau Extract (Premna oblongifolia Merr) and Other Types of Non-Digestible Fibre Using Faecal Fermentation Supernatants and Caco-2 Cells as a Model of the Human Colon

et al. 2017

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Green cincau (Premna oblongifolia Merr) is an Indonesian food plant with a high dietary fibre content. Research has shown that dietary fibre mixtures may be more beneficial for colorectal cancer prevention than a single dietary fibre type. The aim of this study was to investigate the effects of green cincau extract on short chain fatty acid (SCFA) production in anaerobic batch cultures inoculated with human faecal slurries and to compare these to results obtained using different dietary fibre types (pectin, inulin, and cellulose), singly and in combination. Furthermore, fermentation supernatants (FSs) were evaluated in Caco-2 cells for their effect on cell viability, differentiation, and apoptosis. Cincau increased total SCFA concentration by increasing acetate and propionate, but not butyrate concentration. FSs from all dietary fibre sources, including cincau, reduced Caco-2 cell viability. However, the effects of all FSs on cell viability, cell differentiation, and apoptosis were not simply explainable by their butyrate content. In conclusion, products of fermentation of cincau extracts induced cell death, but further work is required to understand the mechanism of action. This study demonstrates for the first time that this Indonesian traditional source of dietary fibre may be protective against colorectal cancer.

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Section: Discussioncontrasting

confidence: 55%

“…Compounds such as short chain fatty acids (SCFAs), which are produced by bacterial fermentation of undigested dietary fibre, are capable of inhibiting cancer in vitro and in vivo [2,3,4,5,6]. The type of dietary fibre consumed influences the proportion and distribution of SCFAs in the gastrointestinal tract [7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Anti-Cancer Effects of Cincau Extract (Premna oblongifolia Merr) and Other Types of Non-Digestible Fibre Using Faecal Fermentation Supernatants and Caco-2 Cells as a Model of the Human Colon

et al. 2017

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“…SCFA transport is regarded as an important factor that regulates colonic fluid balance, absorption of NaCl, and luminal and intracellular pH [40]. The pKa of SCFAs is approximately 4.8 and, thus, most SCFAs are present as anions at physiological pH 6–7 in the lumen of the colon [18, 51]. The carrier-mediated transport of ionized SCFAs into colonic epithelial cells includes (1) Na + -coupled transporters for monocarboxylates (SMCT1, also referred to as SLC5A8), (2) SCFA − /HCO 3 − exchangers (primarily SLC26A3 with the possible additional involvement of SLC26A6), and (3) H + -coupled monocarboxylate transporters (MCT1, MCT4, and MCT5, also referred to as SLC16A1, SLC16A3, and SLC16A4, respectively) [51].…”

Section: Introductionmentioning

confidence: 99%

Involvement of butyrate in electrogenic K+ secretion in rat rectal colon

Inagaki

Hayashi

Andharia

et al. 2018

Pflugers Arch - Eur J Physiol

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Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are synthesized from dietary carbohydrates by colonic bacterial fermentation. These SCFAs supply energy, suppress cancer, and affect ion transport. However, their roles in ion transport and regulation in the intracellular environment remain unknown. In order to elucidate the roles of SCFAs, we measured short-circuit currents (ISC) and performed RT-PCR and immunohistochemical analyses of ion transporters in rat rectal colon. The application of 30 mM butyrate shifted ISC in a negative direction, but did not attenuate the activity of epithelial Na+ channels (ENaC). The application of bumetanide, a Na+-K+-2Cl− cotransporter inhibitor, to the basolateral side reduced the negative ISC shift induced by butyrate. The application of XE991, a KCNQ-type K+ channel inhibitor, to the apical side decreased the ISC shift induced by butyrate in a dose-dependent manner. The ISC shift was independent of HCO3− and insensitive to ibuprofen, an SMCT1 inhibitor. The mucosa from rat rectal colon expressed mRNAs of H+-coupled monocarboxylate transporters (MCT1, MCT4, and MCT5, also referred to as SLC16A1, SLC16A3, and SLC16A4, respectively). RT-PCR and immunofluorescence analyses demonstrated that KCNQ2 and KCNQ4 localized to the apical membrane of surface cells in rat rectal colon. These results indicate that butyrate, which may be transported by H+-coupled monocarboxylate transporters, activates K+ secretion through KCNQ-type K+ channels on the apical membrane in rat rectal colon. KCNQ-type K+ channels may play a role in intestinal secretion and defense mechanisms in the gastrointestinal tract.

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“…That is to say, it acts as a histone deacetylase inhibitor, directly affecting gene expression, and has shown anti-carcinogenic, anti-inflammatory and anti-oxidant functions [15] . It also has an anti-cancer effect on cancer cells, enhancing apoptosis and suppressing proliferation [16] .…”

Section: Fibermentioning

confidence: 99%

Dietary and Lifestyle Factors Associated with Colorectal Cancer Risk and Interactions with Microbiota: Fiber, Red or Processed Meat and Alcoholic Drinks

Tuan

Chen

2015

Gastrointest Tumors

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Background: Diets and lifestyles have been strongly associated with colorectal cancer (CRC).In the past several decades, emerging evidence has suggested that the gut microbiota may have a role in the development of CRC. Its interaction with diets and lifestyles could affect the carcinogenesis of CRC. Summary: This review presents the most recent epidemiologic and experimental evidence of three factors that may convincingly have a role in CRC, including fiber, red or processed meat, and alcohol, focusing on potential mechanisms and their interactions with the gut microbiota. Key Message: High consumption of fiber, low consumption of red or processed red meat as well as minimizing alcohol intake have been associated with a lower risk of CRC. Many microbial metabolites formed from those three substances may mediate the microbial diversity and the composition and abundance of the gut microbiota, which eventually affects the balance between health and disease, including CRC. Practical Implications: Based on our synthetic review, clinicians may probably offer some recommendations and explanations to their patients who may want to modulate their diet and lifestyle to prevent CRC. As an easily modifiable environmental factor, it may be possible that applying dietary or lifestyle intervention could effectively protect against the development of CRC in the future.

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Revisit dietary fiber on colorectal cancer: butyrate and its role on prevention and treatment

Cited by 101 publications

References 93 publications

Analysis of the Anti-Cancer Effects of Cincau Extract (Premna oblongifolia Merr) and Other Types of Non-Digestible Fibre Using Faecal Fermentation Supernatants and Caco-2 Cells as a Model of the Human Colon

Analysis of the Anti-Cancer Effects of Cincau Extract (Premna oblongifolia Merr) and Other Types of Non-Digestible Fibre Using Faecal Fermentation Supernatants and Caco-2 Cells as a Model of the Human Colon

Involvement of butyrate in electrogenic K+ secretion in rat rectal colon

Dietary and Lifestyle Factors Associated with Colorectal Cancer Risk and Interactions with Microbiota: Fiber, Red or Processed Meat and Alcoholic Drinks

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