Revisiting Actin's role in early centrosome separation

Wordeman, Linda; Decarreau, Justin

doi:10.1080/15384101.2015.1130090

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…Furthermore, a ring-like cytoplasmic actin surrounding the spindles gradually forms during the metaphase [ 60 ]. Actin further regulates astral microtubule dynamics, centrosome separation [ 61 ] and the positioning and orientation of mitotic spindles [ 62 , 63 ]. The co-localized distribution (Figure 3A ) between myosin Va and actin indicates their functional connection.…”

Section: Discussionmentioning

confidence: 99%

Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability

Zhong

Dong

et al. 2017

Oncotarget

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Myosin Va, a member of Class V myosin, functions in organelle motility, spindle formation, nuclear morphogenesis and cell motility. The purpose of this study is to explore the expression and localization of myosin Va in testicular cancer and prostate cancer, and its specific roles in tumor progression including cell division, migration and proliferation. We detected myosin Va in testicular and prostate tumor tissues using sqRT-PCR, western blot, and immunofluorescence. Tumor samples showed an increased expression of myosin Va, abnormal actin and myosin Va distribution. Immunofluorescence images during the cell cycle showed that myosin Va tended to gather at cytoplasm during anaphase but co-localized with nucleus during other phases, suggesting the roles of myosin Va in disassembly of spindle microtubule, movement of chromosomes and normal cytokinesis. In addition, multi-nucleation and aberrant nuclear morphology were observed in myosin Va-knockdown cells. Wounding assay and CCK-8-based cell counting were conducted to explore myosin Va roles in cell migration, viability and proliferation. Our results suggest that myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability, and these properties are the hallmark of tumor progression and metastasis development. Therefore, an increased understanding of myosin Va expression and function will assist in the development of future oncodiagnosis and -therapy.

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Section: Discussionmentioning

confidence: 99%

Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability

Zhong

Dong

et al. 2017

Oncotarget

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“…Partial depletion of γ-actin can cause excessive centrosome amplification in cancer cells and delay the mitosis passage [105]. It is obvious that the experimental data obtained in recent years on the centrosome's interaction with the cell γ-actin structures and on the signaling cascades associated with this interaction are universal for cells of various types, including endothelial cells [106]. As we now understand, all cytoskeletal systems are directly or indirectly involved in the implementation of the main function of endothelial cells, and the existence of cross-linker proteins suggests mutual regulation of cytoskeletal components.…”

Section: The Centrosome Of Endotheliocytes Being An Active Center Of ...mentioning

confidence: 99%

The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance

Shakhov,

Churkina,

Kotlobay

et al. 2023

IJMS

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This review summarizes information about the specific features that are characteristic of the centrosome and its relationship with the cell function of highly specialized cells, such as endotheliocytes. It is based on data from other researchers and our own long-term experience. The participation of the centrosome in the functional activity of these cells, including its involvement in the performance of the main barrier function of the endothelium, is discussed. According to modern concepts, the centrosome is a multifunctional complex and an integral element of a living cell; the functions of which are not limited only to the ability to polymerize microtubules. The location of the centrosome near the center of the interphase cell, the concentration of various regulatory proteins in it, the organization of the centrosome radial system of microtubules through which intracellular transport is carried out by motor proteins and the involvement of the centrosome in the process of the perception of the external signals and their transmission make this cellular structure a universal regulatory and distribution center, controlling the entire dynamic morphology of an animal cell. Drawing from modern data on the tissue-specific features of the centrosome’s structure, we discuss the direct involvement of the centrosome in the performance of functions by specialized cells.

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Revisiting Actin's role in early centrosome separation

Cited by 5 publications

References 7 publications

Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability

Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability

The Endothelial Centrosome: Specific Features and Functional Significance for Endothelial Cell Activity and Barrier Maintenance

Further Reading | Centrosome Duplication

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