Revisiting of Properties and Modified Polyethylenimine-Based Cancer Gene Delivery Systems

Mohammadi, Naser; Hosseini, Nashmin Fayazi; Nemati, Hossein; Moradi-Sardareh, Hemen; Nabi‐Afjadi, Mohsen; Kardar, Gholam Ali

doi:10.1007/s10528-023-10416-7

Cited by 5 publications

(3 citation statements)

References 96 publications

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“…Nonviral gene delivery systems must be specially created for the genetic material and cell type to was wholly inhibited at all ratios while the free pDNA moved from the well (Figure 1). of the delivered gene (Mohammadi et al, 2023;Zakeri et al, 2018).…”

Section: Preparation Of Formulationsmentioning

confidence: 99%

“…have a therapeutic effect on target cells(Mohammadi et al, 2023;Ren et al, 2019). To improve the stability, cellular uptake, and intracellular trafficking of pDNA, viral or non-viral gene delivery systems are used.TNBC is one of the cancer cells that express folate receptors, and FA-conjugated polyplexes represent promising vectors in gene therapy targeted against these cells(Jiang et al, 2011; Laemon & Low, 2001;Lu & Low, 2012;Cao et al, 2020;Jahan et al, 2021).This study aimed to develop a non-viral polymericgene delivery system due to LPEI's low toxicity and cost-effectiveness.…”

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confidence: 99%

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Üçlü negatif meme kanseri hücrelerine folik asit polietilenimin polipleksleri ile gen aktarımı

DEMİR DORA

2023

Fabad J Pharm Sci

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Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and lacks hormonal and growth factor receptors commonly expressed by other types of breast cancer making it difficult to treat by conventional treatments. Although gene therapy might be a therapeutic option, delivery of genes into TNBC cells is still an obstacle. In this study, it was aimed to overcome this obstacle by folic acid (FA) conjugated polyplex formulations to targeting the folate receptor which has been reported to be overexpressed in TNBC cells. Non-covalent complexes of FA and LPEI polyplexes (FA-polyplexes) were prepared at six different ratios. After characterization studies, cytotoxicity and transfection ability were evaluated. Conjugation of FA by increasing amounts of LPEI polyplexes, increased the size from 204.1 to 469.8 nm. Their PDI values were between 0.31-0.51, and zeta potentials were positive. After treatment with polyplex formulations, cell viability was decreased significantly starting from 3:1(w/w) polymer:pDNA ratio and from 3:3:1 (w/w)FA:polyplex ratio. Cell viability decreased below 70% above the 5:1 (w/w) polymer:pDNA ratio. Addition of folic acid to polyplex formulations reversed the cytotoxicity of P3, P4 and P5 formulations. Although LV-RFP pDNA was delivered successfully into 4T1 cells by all formulations, fluorescent microscope images showed that, the optimal formulations were FA-P3 and FA-P4. This gene delivery system, generated by non-covalent conjugation of folic acid to polyplexes, increased the uptake and decreased the cytotoxicity of LPEI polyplexes. Non-covalent complexes of folic acid-LPEI polyplexes represent promising delivery systems in gene therapy, directed against cancer cells expressing folate receptors.

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Section: Preparation Of Formulationsmentioning

confidence: 99%

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confidence: 99%

Üçlü negatif meme kanseri hücrelerine folik asit polietilenimin polipleksleri ile gen aktarımı

DEMİR DORA

2023

Fabad J Pharm Sci

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“…Cancer immunotherapy has become a potent disease treatment option that helps advanced cancer patients survive longer while removing any chance of returning tumors [ 1 , 2 ]. In cancer patients, immune cells are ineffective against cancer cells and promote tumor growth, decreasing treatment effectiveness [ 3 , 4 ]. Among innate system cells, macrophages play a crucial role in normal homeostasis, inflammation, and phagocytosis [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Macrophage’s role in solid tumors: two edges of a sword

Jahandideh,

Yarizadeh,

Noei-Khesht Masjedi

et al. 2023

Cancer Cell Int

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The tumor microenvironment is overwhelmingly dictated by macrophages, intimately affiliated with tumors, exercising pivotal roles in multiple processes, including angiogenesis, extracellular matrix reconfiguration, cellular proliferation, metastasis, and immunosuppression. They further exhibit resilience to chemotherapy and immunotherapy via meticulous checkpoint blockades. When appropriately stimulated, macrophages can morph into a potent bidirectional component of the immune system, engulfing malignant cells and annihilating them with cytotoxic substances, thus rendering them intriguing candidates for therapeutic targets. As myelomonocytic cells relentlessly amass within tumor tissues, macrophages rise as prime contenders for cell therapy upon the development of chimeric antigen receptor effector cells. Given the significant incidence of macrophage infiltration correlated with an unfavorable prognosis and heightened resistance to chemotherapy in solid tumors, we delve into the intricate role of macrophages in cancer propagation and their promising potential in confronting four formidable cancer variants—namely, melanoma, colon, glioma, and breast cancers.

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A Review of the Potential Role of CoQ10 in the Treatment of Hepatocellular Carcinoma

Liaghat,

Yaghoubzad-Maleki,

Nabi-Afjadi

et al. 2023

Biochem Genet

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Revisiting of Properties and Modified Polyethylenimine-Based Cancer Gene Delivery Systems

Cited by 5 publications

References 96 publications

Üçlü negatif meme kanseri hücrelerine folik asit polietilenimin polipleksleri ile gen aktarımı

Üçlü negatif meme kanseri hücrelerine folik asit polietilenimin polipleksleri ile gen aktarımı

Macrophage’s role in solid tumors: two edges of a sword

A Review of the Potential Role of CoQ10 in the Treatment of Hepatocellular Carcinoma

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