Revisiting quick, easy, cheap, effective, rugged, and safe parameters for sample preparation in pesticide residue analysis of lettuce by liquid chromatography–tandem mass spectrometry

Konatu, Fernanda Ribeiro Begnini; Breitkreitz, Márcia Cristina; Jardim, Isabel Cristina Sales Fontes

doi:10.1016/j.chroma.2016.12.061

Cited by 38 publications

(10 citation statements)

References 25 publications

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“…Appropriate coefficients of determination (R 2 ) were obtained for the scope of the targeted pesticides, with R 2 in the range of 0.9900-0.9994. The LOQ values were similar to or better than those of previously published works [19][20][21]. The LOQ ranged from 0.77 to 1.79 µg/kg for OCP and 0.57-1.57 µg/kg for OPP.…”

Section: Methods Evaluationsupporting

confidence: 83%

“…The 2019 European Food Safety Authority (EFSA) report on pesticide residues in food [ 18 ] concluded that there is a lack of work in the scientific literature on organic products. There are only a few recent studies that describe chemical safety in terms of pesticides in organic lettuce samples [ 19 , 20 , 21 ]. Similarly, and in accordance with the European food legislation (Regulation (EC) No.…”

Section: Introductionmentioning

confidence: 99%

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Microbiological and Chemical Quality of Portuguese Lettuce—Results of a Case Study

Ferreira

Lopes

Costa

et al. 2020

Foods

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In addition to environmental pollution issues, social concerns about the sustainability, safety, and quality of conventionally grown fruits and vegetables have been increasing. In order to evaluate if there were any microbiological differences between samples of organic and conventional lettuce, a wide range of parameters were tested, including pathogens and indicator organisms: the enumeration of Escherichia coli; the detection of Salmonella spp.; the detection/enumeration of Listeria monocytogenes; the enumeration of lactic acid bacteria, Pseudomonas spp. yeasts and molds, and Enterobacteriaceae. This study also evaluated the chemical safety of the lettuce samples, quantifying the nitrate concentration and 20 pesticides (14 organochlorine and 6 organophosphorus pesticides). Significant differences (p < 0.05) between the conventional and organic samples were only detected for the counts of total microorganisms at 30 °C. Pathogens were absent in all the samples. The analytical method, using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for pesticide extraction, was suitable for detecting the targeted analytes; the limit of quantification (LOQ) was between 0.6 and 1.8 µg/kg (lower than the Maximum Residue Levels (MRLs) established by EU legislation). In three organic lettuce samples, one organochlorine pesticide (α-HCH) was observed below the MRLs. For the samples analyzed and for the parameters investigated, except for the total mesophilic counts, the organic and conventional lettuces were not different.

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Section: Methods Evaluationsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Microbiological and Chemical Quality of Portuguese Lettuce—Results of a Case Study

Ferreira

Lopes

Costa

et al. 2020

Foods

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“…Urine is a liquid sample, so it is appropriate to prepare the sample using simple QuEChERS methods for high extraction efficiency (recovery) of pesticide multiresidues. Since the first QuEChERS method for crops was developed using GC-MS in 2003 [29], there have been preparation procedure improvements for LC-amenable pesticides or lower recovery rate compounds such as pH-dependent pesticides [37,38]. In this study, optimization of the final preparation step was established by comparing the scaled-down methods from three representative QuEChERS procedures [29,30,31].…”

Section: Resultsmentioning

confidence: 99%

A Quantitative Tandem Mass Spectrometry and Scaled-Down QuEChERS Approach for Simultaneous Analysis of Pesticide Multiresidues in Human Urine

et al. 2019

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Multiresidual pesticide determination in a biological sample is essential for an immediate decision and response related to various pesticide intoxications. A rapid and simultaneous analytical method for 260 pesticides in human urine was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). High speed positive/negative switching electrospray ionization (ESI) mode was used, and scheduled multiple reaction monitoring (MRM) was optimized. Three versions of scaled-down QuEChERS procedures were evaluated, and the procedure using non-buffer reagents (magnesium sulfate and sodium chloride) and excluding cleanup steps was selected for optimum pesticide extraction. The limit of quantitation (LOQ) in this methodology was 10 ng/mL for each target pesticide, and correlation coefficient (r2) values of calibration curves were ≥0.988 (linearity range; 10–250 ng/mL). In accuracy and precision tests, the relative error ranges were −18.4% to 19.5%, with relative standard deviation (RSD) 2.1%–19.9% at an LOQ level (10 ng/mL), and −14.7% to 14.9% (RSD; 0.6%–14.9%) at higher concentrations (50, 150, and 250 ng/mL). Recovery range was 54.2%–113.9% (RSD; 0.3%–20.0%), and the soft matrix effect (range; −20% to 20%) was observed in 75.4% of target pesticides. The established bioanalytical methods are sufficient for application to biomonitoring in agricultural exposures and applicable in the forensic and clinic.

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“…Giuliana Salvador Tomasin 28 amostras de alimentos (AGUIAR JÚNIOR et al, 2020;SONG et al, 2018;KONATU et al, 2017;GUAN et al, 2010;FERNANDES et al, 2014). No entanto, esse número não é tão expressivo quanto ao uso da DPX em determinações de metais, sendo encontradas publicações envolvendo a determinação de paládio em resíduos líquidos (JAILSON et al, 2012 ) 238,2008).…”

Section: Dissertação De Mestradounclassified

“…aspiração do solvente de eluição, seguido da aspiração de ar, várias vezes (até completa dessorção dos analitos). O eluato pode ser analisado diretamente ou evaporado e reconstituído (PINTO; QUEIROZ, 2015).Nos últimos 10 anos, a extração por DPX tem sido reportada com muitas aplicações na determinação de diversos compostos orgânicos, tais como: pesticidas(CORAZZA et al, 2019e OENNING et al, 2018KONATU et al, 2017), inseticidas (SONG et al, 2018, fungicida(AGUIAR et al, 2020), fármacos (MAFRA et al, 2018, drogas de abuso(ELLISON et al, 2009), contaminantes emergentes orgânicos(MORÉS et al. 2019) em amostras com diferentes…”

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Desenvolvimento de procedimento DPX para determinação sensível de Cu(II) em água para hemodiálise por F AAS utilizando sementes de Moringa oleifera como fase sólida

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Revisiting quick, easy, cheap, effective, rugged, and safe parameters for sample preparation in pesticide residue analysis of lettuce by liquid chromatography–tandem mass spectrometry

Cited by 38 publications

References 25 publications

Microbiological and Chemical Quality of Portuguese Lettuce—Results of a Case Study

Microbiological and Chemical Quality of Portuguese Lettuce—Results of a Case Study

A Quantitative Tandem Mass Spectrometry and Scaled-Down QuEChERS Approach for Simultaneous Analysis of Pesticide Multiresidues in Human Urine

Desenvolvimento de procedimento DPX para determinação sensível de Cu(II) em água para hemodiálise por F AAS utilizando sementes de Moringa oleifera como fase sólida

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