Revisiting the Assignment of Rv0241c to Fatty Acid Synthase Type II ofMycobacterium tuberculosis

Abstract

The fatty acid synthase type II enzymatic complex of Mycobacterium tuberculosis (FAS-II

Biochemical Characterization of Isoniazid-resistant Mycobacterium tuberculosis: Can the Analysis of Clonal Strains Reveal Novel Targetable Pathways?

“…CoA substrates rather than ACP derivatives, which gives more support to our hypothesis [64]. Finally, the fatty-acid-AMP ligase FadD13 was significantly increased in the clinical INHr strain (supplemental Fig.…”

A new dehydratase conferring innate resistance to thiacetazone and intra‐amoebal survival of Mycobacterium smegmatis

“…Because MSMEG_6754 shows considerable primary sequence conservation with the FAS‐II HadABC dehydratases, we investigated whether their in vivo activities were also similar. The M. smegmatis ΔMSMEG_6754 mutant was transformed with plasmids containing either M. tuberculosis hadABC or Rv0241 , which encodes a dehydratase from M. tuberculosis with a strict dependence on CoA as opposed to the ACP used by HadAB (Sacco et al ., ). While the mutant containing pMV261_ MtbhadABC grew on agar plates containing high concentrations of TAC (up to 100 μg ml −1 ), similar to the MSMEG_6754‐complemented strain (Fig.…”

Crystal structure of FabG4 from Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity

“…In M. tuberculosis the dehydratase gene (Rv0241c) is overexpressed during the organism's exposure to the inhibitory conditions of fatty acid synthesis (Boshoff et al, 2004). The dehydratase gene is recently shown to introduce unsaturation on the fatty acyl backbone once the hydroxyl group is present on the b carbon (Sacco et al, 2010). Since FabG4 can incorporate hydroxyl group on the same b carbon, these two proteins may be related to a single pathway.…”