Revisiting the mechanisms of copper toxicity to rainbow trout: Time course, influence of calcium, unidirectional Na+ fluxes, and branchial Na+, K+ ATPase and V-type H+ ATPase activities

Chowdhury, M. Jasim; Girgis, Mina; Wood, Chris M.

doi:10.1016/j.aquatox.2016.05.009

Cited by 30 publications

(6 citation statements)

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“…Aerobic ATP production is essential for driving the active transport of ions mediated by pumps and co-transporters in osmoregulatory tissues (Marshall, 2002). In freshwater fish, Cu can inhibit critical enzymes involved in Na + uptake, such as Na + /K + ATPase, v-type H + -ATPase, Na + /H + exchanger, and CA (Chowdhury et al, 2016;Grosell et al, 2012). Our findings revealed that, although both Cu and nCuO are ionoregulatory toxicants to dwarf cichlid, nCuO might be more effective in promoting ionoregulatory disturbances in this species.…”

Section: Dwarf Cichlid and Cardinal Tetra Exhibit Different Osmoregulmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In rainbow trout, acute Cu exposure has negligible effects on whole animal oxygen consumption (ṀO 2 ) (De Boeck et al, 2006), but consistently stimulates diffusive Na + losses and inhibits Na + uptake (Chowdhury et al, 2016). The latter responses were directly linked to the inhibition of gill Na + /K + -ATPase, V-type H + -ATPase and carbonic anhydrase (CA) (Chowdhury et al, 2016). In contrast, common carp (Cyprinus carpio) and gibel carp (Carassius auratus gibel) exposed to Cu exhibited a decline in ṀO 2 , suggesting that the disruption of aerobic metabolism might be a key factor for acute toxicity in these species (De Boeck et al, 1995, 2006.…”

Section: Introductionmentioning

confidence: 99%

“…Exposure of cardinal tetra to similar levels of Cu used in this study (around 50 μg L −1 ) resulted in the stimulation of Na + , Cl − and K + losses (Crémazy et al, 2016). Cu induced imbalances in Na + homeostasis of freshwater fishes is demonstrated to involve both the stimulation of Na + paracellular losses, by the displacement of Ca 2+ ions from branchial tight junctions, and inhibition of Na + influx through the competition for Na + channels in apical membrane of ionocytes, and/or inhibition of different transporters, as CA, v-type H + -ATPase and Na + / K + -ATPase (Alsop and Wood, 2011;Chowdhury et al, 2016;Crémazy et al, 2016;Grosell et al, 2002). In these regards, it has been proposed that the specialized high branchial Na + uptake system seen in cardinal tetra (Gonzalez and Wilson, 2001) may explain its tolerance to both high H + or Cu levels conditions (Crémazy et al, 2016).…”

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confidence: 99%

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Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)

Braz-Mota

Campos

MacCormack

et al. 2018

Science of The Total Environment

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Copper oxide nanoparticles (nCuO) are widely used in boat antifouling paints and are released into the environment, potentially inducing toxicity to aquatic organisms. The present study aimed to understand the effects of nCuO and dissolved copper (Cu) on two ornamental Amazon fish species: dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi). Fish were exposed to 50% of the LC for nCuO (dwarf cichlid 58.31μgL and cardinal tetra 69.6μgL) and Cu (dwarf cichlid 20μgL and cardinal tetra 22.9μgL) for 24, 48, 72 and 96h. Following exposure, aerobic metabolic rate (ṀO), gill osmoregulatory physiology and mitochondrial function, oxidative stress markers, and morphological damage were evaluated. Our results revealed species specificity in metabolic stress responses. An increase of ṀO was noted in cardinal tetra exposed to Cu, but not nCuO, whereas ṀO in dwarf cichlid showed little change with either treatment. In contrast, mitochondria from dwarf cichlid exhibited increased proton leak and a resulting decrease in respiratory control ratios in response to nCuO and Cu exposure. This uncoupling was directly related to an increase in reactive oxygen species (ROS) levels. Our findings reveal different metabolic responses between these two species in response to nCuO and Cu, which are probably caused by the differences between species natural histories, indicating that different mechanisms of toxic action of the contaminants are associated to differential osmoregulatory strategies among species.

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Section: Dwarf Cichlid and Cardinal Tetra Exhibit Different Osmoregulmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)

Braz-Mota

Campos

MacCormack

et al. 2018

Science of The Total Environment

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“…Copper compounds can be effective (Watton and Hawkes 1984;Dwyer et al 2003), but serious latent and long-term effects on the environment can result from their use. Elevated copper concentrations have been demonstrated to have serious physiological and biochemical effects on many freshwater organisms and their food sources (De Boeck et al 2003;Ahlf et al 2009;McIntyre et al 2015;Chowdhury et al 2016;Ghosh et al 2016;Sharley et al 2016). Other chemical tools include quaternary and polyquaternary compounds and microencapsulated ammonium chlorides (Britton and Dingman 2011;Calazans et al 2013;Stout et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Aqueous Alkaline Solutions to New Zealand Mudsnails, Asian Clams, and Quagga Mussels

Barenberg

2017

Journal of Fish and Wildlife Management

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We modeled the toxicity response of three important aquatic nuisance species to alkaline solutions of pH 12 to explore the potential use of chemical treatments for decontaminating infested water distribution systems, tanks, and other facilities in confined freshwater environments. Groups of quagga mussels Dreissena bugensis, New Zealand mudsnails Potamopyrgus antipodarum, and Asian clams Corbicula fluminea were tested in temperatures from 19 to 22°C in aqueous solutions adjusted to pH 12 with NaOH. The predicted 99% lethal exposure time (LT99) for adult-sized quagga mussels and New Zealand mudsnails respectively was 31.3 and 35.1 h. Longer exposures were needed to kill adult-sized Asian clams (LT99 = 208.9 h). Because of the worldwide use of NaOH as an alkaline reagent in a variety of industrial and pesticide applications, and its ease of neutralization with CO2, elevating the pH of freshwater solutions with NaOH may be a practicable management tool to remove nuisance species in biofouled canals, pipes, hatchery tanks, and other confined structures.

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Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Kumar,

Gismondi,

Reddy

2023

Environmental Toxicology

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The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu‐NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu‐NPs were determined as 8.04 and 3.85 mg L−1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L−1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L−1 for Cu‐NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione‐s‐transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu‐NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu‐NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu‐NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu‐NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu‐NPs, induce toxicity and disrupt cellular metabolic activities in fish.

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Revisiting the mechanisms of copper toxicity to rainbow trout: Time course, influence of calcium, unidirectional Na+ fluxes, and branchial Na+, K+ ATPase and V-type H+ ATPase activities

Cited by 30 publications

References 63 publications

Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)

Mechanisms of toxic action of copper and copper nanoparticles in two Amazon fish species: Dwarf cichlid (Apistogramma agassizii) and cardinal tetra (Paracheirodon axelrodi)

Toxicity of Aqueous Alkaline Solutions to New Zealand Mudsnails, Asian Clams, and Quagga Mussels

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

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