We calculate the chromomagnetic dipole moment of the top quark, μt, in the context of the Bestest Little Higgs model. This extension of the Standard Model aims to solve the hierarchy problem without fine tuning, by introducing one-loop corrections to the Higgs boson mass through heavy top quark partners and heavy gauge bosons. We found that the largest resulting value for the chromomagnetic is μt ∼ 10 −5 and the lowest is around 10 −6 , mainly due to the Higgs boson of the Standard Model, which couples to both the top quark of the Standard Model and its heavy partners. Also, we present a wide variety of new Feynman rules involved in our calculation.