Revisiting the urease production of MICP-relevant bacterium Sporosarcina pasteurii during cultivation

Lapierre, Frédéric M.; Huber, Robert

doi:10.1016/j.bcab.2023.102981

Biocatalysis and Agricultural Biotechnology

2024

DOI: 10.1016/j.bcab.2023.102981

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Revisiting the urease production of MICP-relevant bacterium Sporosarcina pasteurii during cultivation

Frédéric M. Lapierre,

Robert Huber

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Cited by 5 publications

References 29 publications

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Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP

Lapierre,

Huber

2024

Biotechnology Journal

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The bacterium Sporosarcina pasteurii is the most commonly used microorganism for Microbial Induced Calcite Precipitation (MICP) due to its high urease activity. To date, no proper fed‐batch cultivation protocol for S. pasteurii has been published, even though this cultivation method has a high potential for reducing costs of producing microbial ureolytic biomass. This study focusses on fed‐batch cultivation of S. pasteurii DSM33. The study distinguishes between limited fed‐batch cultivation and extended batch cultivation. Simply feeding glucose to a S. pasteurii culture does not seem beneficial. However, it was exploited that S. pasteurii is auxotrophic for two vitamins and amino acids. Limited fed‐batch cultivation was accomplished by feeding the necessary vitamins or amino acids to a culture lacking them. Feeding nicotinic acid to a nicotinic acid deprived culture resulted in a 24% increase of the specific urease activity compared to a fed culture without nicotinic acid limitation. Also, extended batch cultivation was explored. Feeding a mixture of glucose and yeast extract results in OD600 of ≈70 at the end of cultivation, which is the highest value published in literature so far. These results have the potential to make MICP applications economically viable.

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Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP

Lapierre,

Huber

2024

Biotechnology Journal

Self Cite

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Medium optimization and dust suppression performance analysis of microbial-based dust suppressant compound by response surface curve method

Hu,

Yang,

Zhao

et al. 2024

Environ Sci Pollut Res

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Enhanced biological treatment of sandy soils through the application of chicken manure as a supplementation material

Sangdeh,

Zanganeh Ranjbar,

Payan

et al. 2024

Results in Engineering

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Revisiting the urease production of MICP-relevant bacterium Sporosarcina pasteurii during cultivation

Cited by 5 publications

References 29 publications

Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP

Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP

Medium optimization and dust suppression performance analysis of microbial-based dust suppressant compound by response surface curve method

Enhanced biological treatment of sandy soils through the application of chicken manure as a supplementation material

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