Revitalization of Tooth with Necrotic Pulp and Open Apex by Using Platelet-rich Plasma: A Case Report

Torabinejad, Mahmoud; Turman, Michael

doi:10.1016/j.joen.2010.11.004

Cited by 268 publications

(283 citation statements)

References 34 publications

(101 reference statements)

Supporting

Mentioning

246

Contrasting

Unclassified

Order By: Relevance

“…There is a growing body of evidence from case reports to suggest that regenerative endodontics may in fact be possible in teeth with pulpal necrosis, apical pathosis, and immature apices (6)(7)(8)(9). This treatment procedure has acquired many names such as pulp revitalization, root canal revascularization, and regenerative endodontic treatment (RET) (2, 9-11).…”

Section: Introductionmentioning

confidence: 99%

“…The most frequent pretreatment diagnosis of these cases was of pulpal necrosis with or without apical periodontitis. Postoperative recalls showed regression of clinical symptoms in addition to radiographic evidence of hard tissue formation and apical closure (6)(7)(8)(9)(10)12). However, the application of nonstandardized 2-dimensional (2D) radiographs to evaluate an increase in root length and dentin thickness should be interpreted with caution because a slight change in the angulation at the preoperative or recall appointment might produce inconsistent images and inaccurate interpretations (12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3-dimensional Analysis of Regenerative Endodontic Treatment Outcome

EzEldeen

Gorp

Dessel

et al. 2015

Journal of Endodontics

View full text Add to dashboard Cite

Introduction: A growing body of evidence supports the regeneration potential of dental tissues after regenerative endodontic treatment (RET). Nevertheless, a standard method for the evaluation of RET outcome is lacking. The aim of this study was to develop a standardized quantitative method for RET outcome analysis based on cone-beam computed tomographic (CBCT) volumetric measurements. Methods: Five human teeth embedded in mandibular bone samples were scanned using both an Accuitomo 170 CBCT machine (Morita, Kyoto, Japan) and a SkyScan 1174 micro-computed tomographic (mCT) system (SkyScan, Antwerp, Belgium). For subsequent clinical application, clinical data and low-dose CBCT scans (preoperatively and follow-up) from 5 immature permanent teeth treated with RET were retrieved. In vitro and clinical 3-dimensional image data sets were imported into a dedicated software tool. Two segmentation steps were applied to extract the teeth of interest from the surrounding tissue (livewire) and to separate tooth hard tissue and root canal space (level set methods). In vitro and clinical volumetric measurements were assessed separately for differences using Wilcoxon matched pairs test. Pearson correlation analysis and Bland-Altman plots were used to evaluate the relation and agreement between the segmented CBCT and mCT volumes. Results: The results showed no statistical differences and strong agreement between CBCT and mCT volumetric measurements. Volumetric comparison of the root hard tissue showed significant hard tissue formation. (The mean volume of newly formed hard tissue was 27.9 [AE10.5] mm 3 [P < .05]). Conclusions: Analysis of 3-dimensional data for teeth treated with RET offers valuable insights into the treatment outcome and patterns of hard tissue formation. (J Endod 2015;-:1-8)

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3-dimensional Analysis of Regenerative Endodontic Treatment Outcome

EzEldeen

Gorp

Dessel

et al. 2015

Journal of Endodontics

View full text Add to dashboard Cite

show abstract

“…Além dos estudos in vitro; a ação antimicrobiana desta pasta composta por antibióticos tem sido relatada in vivo em estudos com cães (Windley et al, 2005;Wang et al, 2010) e estudos clínicos (Petrino, 2007;Jung et al, 2008;Shah et al, 2008;Ding et al, 2009;Reynolds et al, 2009;Kim et al, 2010;Petrino et al, 2010;Thomson;Kahler, 2010;Nosrat et al, 2011;Turman, 2011;Jeeruphan et al, 2012;Lenzi;Trope, 2012;Nosrat et al, 2012).…”

Section: Medicações Intracanalunclassified

“…A maioria dos protocolos propostos na literatura envolve a manipulação dos tecidos periapicais, com o objetivo de promover o sangramento para induzir a formação de um coágulo sanguíneo no espaço do canal radicular (Banchs;Trope, 2004;Huang, 2006;Petrino, 2007;Thibodeau;Cotti et al, 2008;Jung et al, 2008;Shah et al, 2008;Ding et al, 2009;Reynolds et al, 2009;Shin et al, 2009;Thibodeau, 2009;Kim et al, 2010;Petrino et al, 2010;Thomson;Kahler, 2010;Cehreli et al, 2011;Nosrat et al, 2011;Chen et al, 2012;Jeeruphan et al, 2012;Lenzi;Trope, 2012;Nosrat et al, 2012;Soares et al, 2013;Nagata et al, 2014a,b Turman, 2011).…”

Section: Scaffolfd-coágulo Sanguíneounclassified

“…Há também outros tipos de "scaffolds" biodegradáveis ou permanentes disponíveis como colágeno, ácido hialurônico ou sintéticos como o ácido poliglicólico ou hidroxiapatita (Gotlieb et al, 2008;Chandrahasa et al, 2011 (Banchs;Trope, 2004;Petrino, 2007;Thibodeau;Cotti et al, 2008;Jung et al, 2008;Ding et al, 2009;Reynolds et al, 2009;Shin et al, 2009;Thibodeau, 2009;Kim et al, 2010;Petrino et al, 2010;Thomson;Kahler, 2010;Cehreli et al, 2011;Turman, 2011;Chen et al, 2012;Jeeruphan et al, 2012;Lenzi;Trope, 2012;Nosrat et al, 2012). Nosrat et al (2011) utilizaram uma mistura enriquecida de cálcio (CEM), uma substância que tem características de selamento e biocompatibilidade semelhantes ao MTA, é capaz de promover a formação de hidroxiapatita, induzir diferenciação de células-tronco e induzir a formação de tecido mineralizado (Asgary et al, 2009 (Lovelace et al, 2011).…”

Section: Scaffolfd-coágulo Sanguíneounclassified

See 1 more Smart Citation

Influência de medicações intracanal utilizadas em procedimentos endodônticos regenerativos na sobrevivência de células da papila apical in vitro

Oliveira¹

View full text Add to dashboard Cite

Oliveira AP. Influence of intracanal medications used in regenerative endodontic procedures on survival of apical papilla cells in vitro [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2014. Versão Corrigida.Regenerative Endodontic procedures have provided changes in treatment of patients with immature teeth and apical periodontitis enabling full root development and lower incidence of dental fracture. Intracanal dressings are used for disinfection; however, their effect on apical papilla cells is poorly elucidated. Additionally, the effect of these substances on cells previously subjected to proinflammatory condition is still unknown. The aim of this study was to evaluate the cytotoxicity of intracanal dressings used in Endodontics regenerative procedures on cultured human apical papilla cells at physiologic and activated condition. Cell culture was established from the apical papilla removed from an extracted immature third molar. The substances studied were triple antibiotic modified paste: ciprofloxacin, metronidazole and cephalosporin (1:1:1); CFC: ciprofloxacin, metronidazole and calcium hydroxide (1:1:2) and modified CFC: ciprofloxacin , metronidazole and calcium hydroxide (2:2:1). Part of the cells was stimulated previously with lipotheichoic acid (LTA) of Enterococcus faecalis por 7 days. Once plated, cells were exposed to increasing concentration of the medications for 1, 3, 5 and 7 days. Cell viability was evaluated by means of 3-bromide (4.5-dimetiliazol-2-yl) -2.5-difeniltetraze (MTT) and Nitric Oxide (NO) release was assessed by the Griess method. The statistical analysis was done through analysis of variance with 1 criteria (ANOVA) followed by Tukey test with 5% of significance level. Modified CFC was the medication that demonstrated the less cytotoxic effect on cell viabilityat the experimental periods studied while CFC promoted significant decrease on cell viability specially after 7 days of contact. The modified triple antibiotic paste resulted in important alteration of cell viability being considered the most citotoxic. Cellular activation by LTA resulted in increased levels of mitochondrial activity for all medications being more evident at the longer experimental periods. Cellular activation also contributed to higher levels of nitric oxide release. In conclusion, the cytotoxic effect of the tested medications is dependent on concentration, time of contact and cellular condition, being the triple antibiotic modified paste the most cytotoxic in high concentrations leading clinically in the decreased of the cells viability of the apical papilla, decreasing the success of regenerative procedures.

show abstract