Reviving the RNA World: An Insight into the Appearance of RNA Methyltransferases

Rana, Ajay Kumar; Ankri, Serge

doi:10.3389/fgene.2016.00099

Cited by 37 publications

(37 citation statements)

References 108 publications

(120 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Methylation of the ribose ring in the 2-O position has never been found in simple natural carbohydrates,yet is found in all major classes of RNA, [5,6] and has been hypothesized as the evolutionary link between RNAand DNA. [5,7] Thediscovery of this new arsenylated 2-O-methylriboside could again stimulate speculation about ab iological role for arsenic in early life.…”

contrasting

confidence: 64%

A 2‐O‐Methylriboside Unknown Outside the RNA World Contains Arsenic

et al. 2017

View full text Add to dashboard Cite

Lipid-soluble arsenic compounds, also called arsenolipids, are ubiquitous marine natural products of currently unknown origin and function. In our search for clues about the possible biological roles of these compounds, we investigated arsenic metabolism in the unicellular green alga Dunaliella tertiolecta, and discovered an arsenolipid fundamentally different from all those previously identified; namely, a phytyl 5-dimethylarsinoyl-2-O-methyl-ribofuranoside. The discovery is of particular interest because 2-O-methylribosides have, until now, only been found in RNA. We briefly discuss the significance of the new lipid in biosynthesis and arsenic biogeochemical cycling.

show abstract

contrasting

confidence: 64%

A 2‐O‐Methylriboside Unknown Outside the RNA World Contains Arsenic

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Among these various chemical reactions, the most frequent is the transfer of a methyl (CH 3 ) group from a methylation donor (generally S -adenosyl- l -methionine, abbreviated as SAM or AdoMet) to different positions on nucleobases or to the 2′-OH of the ribose [1,2,3,4,5]. Methylated nucleotides are found in every studied RNA species, and their presence allows fine modulation (tuning) of RNA properties in different cellular processes [6,7,8,9].…”

Section: Introductionmentioning

confidence: 99%

Next‐Generation Sequencing‐Based RiboMethSeq Protocol for Analysis of tRNA 2′‐O‐Methylation

et al. 2017

View full text Add to dashboard Cite

Analysis of RNA modifications by traditional physico-chemical approaches is labor intensive, requires substantial amounts of input material and only allows site-by-site measurements. The recent development of qualitative and quantitative approaches based on next-generation sequencing (NGS) opens new perspectives for the analysis of various cellular RNA species. The Illumina sequencing-based RiboMethSeq protocol was initially developed and successfully applied for mapping of ribosomal RNA (rRNA) 2′-O-methylations. This method also gives excellent results in the quantitative analysis of rRNA modifications in different species and under varying growth conditions. However, until now, RiboMethSeq was only employed for rRNA, and the whole sequencing and analysis pipeline was only adapted to this long and rather conserved RNA species. A deep understanding of RNA modification functions requires large and global analysis datasets for other important RNA species, namely for transfer RNAs (tRNAs), which are well known to contain a great variety of functionally-important modified residues. Here, we evaluated the application of the RiboMethSeq protocol for the analysis of tRNA 2′-O-methylation in Escherichia coli and in Saccharomyces cerevisiae. After a careful optimization of the bioinformatic pipeline, RiboMethSeq proved to be suitable for relative quantification of methylation rates for known modified positions in different tRNA species.

show abstract

“…Dies geschah vor ca. 2,3 Milliarden Jahren als es zeitgleich zu einer langsamen Zunahme des Sauerstoffs in der Erdatmosphäre und somit einer Änderung von reduzierenden zu oxidierenden Bedingungen kam.…”

Section: Ein Alter Mechanismusunclassified

DNA‐Methylierung – das 5. Element

Kuhlmann

2019

Biologie in unserer Zeit

View full text Add to dashboard Cite

Zusammenfassung DNA‐Methylierung ist eine evolutionär hoch konservierte epigenetische Modifikation. Sie ist wichtig für die genomische Stabilität und kann einen Einfluss auf die Transkriptionsaktivität von Genen haben. In Tieren findet man Cytosinmethylierung im symmetrischen CG‐Sequenzkontext, in Pflanzen kann jedes Cytosin potenziell methyliert werden. Die Methylierung im symmetrischen Kontext ist vererbbar, im asymmetrischen Sequenzkontext muss sie jedes Mal neu „geschrieben“ werden. Der Mechanismus hierfür wird als RNA‐dirigierte DNA‐Methylierung bezeichnet. In Pflanzen erfolgt eine Demethylierung unter Zuhilfenahme des DNA‐Reparaturmechanismus. Eine Änderung im Methylierungsmuster kann zu phänotypischen Veränderungen führen. Man spricht hier von Epimutationen. Als Epigenetik wird der Einfluss von stabil vererbbaren Methylierungszuständen ohne DNA‐Sequenzänderung bezeichnet. Die Änderung der Methylierung ist einerseits vererbbar, andererseits jedoch reversibel. Somit besitzt der Organismus mit diesem „5. Element“ die Möglichkeit, eine Mutation sozusagen auszuprobieren oder abgeschwächte Formen einer veränderten Genexpression zu testen.

show abstract

Reviving the RNA World: An Insight into the Appearance of RNA Methyltransferases

Cited by 37 publications

References 108 publications

A 2‐O‐Methylriboside Unknown Outside the RNA World Contains Arsenic

A 2‐O‐Methylriboside Unknown Outside the RNA World Contains Arsenic

Next‐Generation Sequencing‐Based RiboMethSeq Protocol for Analysis of tRNA 2′‐O‐Methylation

DNA‐Methylierung – das 5. Element

Contact Info

Product

Resources

About